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- W4385295556 abstract "An aqueous two-phase system (ATPS) encapsulated within a vesicle organizes the vesicle core as two coexisting phases that partition encapsulated solutes. Here, we use microfluidic technologies to produce vesicles that efficiently encapsulate mixtures of macromolecules, providing a versatile platform to determine the phase behavior of ATPSs. Moreover, we use compartmentalized vesicles to investigate how membrane permeability affects the dynamics of the encapsulated ATPS. Designing a membrane selectively permeable to one of the components of the ATPS, we show that out-of-equilibrium phase separations formed by a rapid outflow of water can be spontaneously reversed by a slower outflow of the permeating component across the vesicle membrane. This dynamics may be exploited advantageously by cells to separate and connect metabolic and signaling routes within their nucleoplasm or cytoplasm depending on external conditions." @default.
- W4385295556 created "2023-07-28" @default.
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- W4385295556 date "2023-07-27" @default.
- W4385295556 modified "2023-10-13" @default.
- W4385295556 title "Aqueous Two-Phase Systems within Selectively Permeable Vesicles" @default.
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- W4385295556 doi "https://doi.org/10.1021/acsmacrolett.3c00341" @default.
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