FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4385299304> ?p ?o ?g. }

• W4385299304 endingPage "e1011032" @default.
• W4385299304 startingPage "e1011032" @default.
• W4385299304 abstract "Seasonal “common-cold” human coronaviruses are widely spread throughout the world and are mainly associated with mild upper respiratory tract infections. The emergence of highly pathogenic coronaviruses MERS-CoV, SARS-CoV, and most recently SARS-CoV-2 has prompted increased attention to coronavirus biology and immunopathology, but the T-cell response to seasonal coronaviruses remains largely uncharacterized. Here we report the repertoire of viral peptides that are naturally processed and presented upon infection of a model cell line with seasonal coronavirus OC43. We identified MHC-bound peptides derived from each of the viral structural proteins (spike, nucleoprotein, hemagglutinin-esterase, membrane, and envelope) as well as non-structural proteins nsp3, nsp5, nsp6, and nsp12. Eighty MHC-II bound peptides corresponding to 14 distinct OC43-derived epitopes were identified, including many at very high abundance within the overall MHC-II peptidome. Fewer and less abundant MHC-I bound OC43-derived peptides were observed, possibly due to MHC-I downregulation induced by OC43 infection. The MHC-II peptides elicited low-abundance recall T-cell responses in most donors tested. In vitro assays confirmed that the peptides were recognized by CD4+ T cells and identified the presenting HLA alleles. T-cell responses cross-reactive between OC43, SARS-CoV-2, and the other seasonal coronaviruses were confirmed in samples of peripheral blood and peptide-expanded T-cell lines. Among the validated epitopes, spike protein S 903-917 presented by DPA1*01:03/DPB1*04:01 and S 1085-1099 presented by DRB1*15:01 shared substantial homology to other human coronaviruses, including SARS-CoV-2, and were targeted by cross-reactive CD4 T cells. Nucleoprotein N 54-68 and hemagglutinin-esterase HE 128-142 presented by DRB1*15:01 and HE 259-273 presented by DPA1*01:03/DPB1*04:01 are immunodominant epitopes with low coronavirus homology that are not cross-reactive with SARS-CoV-2. Overall, the set of naturally processed and presented OC43 epitopes comprise both OC43-specific and human coronavirus cross-reactive epitopes, which can be used to follow CD4 T-cell cross-reactivity after infection or vaccination, and to guide selection of epitopes for inclusion in pan-coronavirus vaccines." @default.
• W4385299304 created "2023-07-28" @default.
• W4385299304 creator A5013927284 @default.
• W4385299304 creator A5037247680 @default.
• W4385299304 creator A5037260292 @default.
• W4385299304 creator A5054495834 @default.
• W4385299304 creator A5061054416 @default.
• W4385299304 creator A5077360176 @default.
• W4385299304 creator A5079255512 @default.
• W4385299304 date "2023-07-27" @default.
• W4385299304 modified "2023-09-30" @default.
• W4385299304 title "Immunopeptidome profiling of human coronavirus OC43-infected cells identifies CD4 T-cell epitopes specific to seasonal coronaviruses or cross-reactive with SARS-CoV-2" @default.
• W4385299304 cites W1502565270 @default.
• W4385299304 cites W1558088803 @default.
• W4385299304 cites W1839525253 @default.
• W4385299304 cites W1964053616 @default.
• W4385299304 cites W1964653209 @default.
• W4385299304 cites W1965031244 @default.
• W4385299304 cites W1977492930 @default.
• W4385299304 cites W1979873794 @default.
• W4385299304 cites W1989457084 @default.
• W4385299304 cites W2010005720 @default.
• W4385299304 cites W2016029872 @default.
• W4385299304 cites W2022990363 @default.
• W4385299304 cites W2023409197 @default.
• W4385299304 cites W2033521803 @default.
• W4385299304 cites W2036196139 @default.
• W4385299304 cites W2037980094 @default.
• W4385299304 cites W2041352768 @default.
• W4385299304 cites W2049110710 @default.
• W4385299304 cites W2060791849 @default.
• W4385299304 cites W2076455712 @default.
• W4385299304 cites W2081779519 @default.
• W4385299304 cites W2085105126 @default.
• W4385299304 cites W2088727990 @default.
• W4385299304 cites W2091376804 @default.
• W4385299304 cites W2102109092 @default.
• W4385299304 cites W2104474203 @default.
• W4385299304 cites W2124995875 @default.
• W4385299304 cites W2125402117 @default.
• W4385299304 cites W2137768688 @default.
• W4385299304 cites W2141504923 @default.
• W4385299304 cites W2149597135 @default.
• W4385299304 cites W2153684517 @default.
• W4385299304 cites W2153771979 @default.
• W4385299304 cites W2159959024 @default.
• W4385299304 cites W2161109832 @default.
• W4385299304 cites W2169150541 @default.
• W4385299304 cites W225152415 @default.
• W4385299304 cites W2306794997 @default.
• W4385299304 cites W2339882081 @default.
• W4385299304 cites W2605830019 @default.
• W4385299304 cites W2792545913 @default.
• W4385299304 cites W2886618560 @default.
• W4385299304 cites W2889452649 @default.
• W4385299304 cites W2898389621 @default.
• W4385299304 cites W2912018962 @default.
• W4385299304 cites W2942123686 @default.
• W4385299304 cites W2950797298 @default.
• W4385299304 cites W2953065444 @default.
• W4385299304 cites W2954376662 @default.
• W4385299304 cites W3016494012 @default.
• W4385299304 cites W3024570138 @default.
• W4385299304 cites W3025774621 @default.
• W4385299304 cites W3045971896 @default.
• W4385299304 cites W3047159181 @default.
• W4385299304 cites W3080428800 @default.
• W4385299304 cites W3087225540 @default.
• W4385299304 cites W3088579978 @default.
• W4385299304 cites W3089759643 @default.
• W4385299304 cites W3091344371 @default.
• W4385299304 cites W3091791643 @default.
• W4385299304 cites W3093824751 @default.
• W4385299304 cites W3093885371 @default.
• W4385299304 cites W3094199989 @default.
• W4385299304 cites W3101669111 @default.
• W4385299304 cites W3108728530 @default.
• W4385299304 cites W3122458452 @default.
• W4385299304 cites W3128254355 @default.
• W4385299304 cites W3141087105 @default.
• W4385299304 cites W3143938824 @default.
• W4385299304 cites W3145651106 @default.
• W4385299304 cites W3152739191 @default.
• W4385299304 cites W3156232187 @default.
• W4385299304 cites W3157113261 @default.
• W4385299304 cites W3158809198 @default.
• W4385299304 cites W3159349374 @default.
• W4385299304 cites W3160504045 @default.
• W4385299304 cites W3161468411 @default.
• W4385299304 cites W3161512998 @default.
• W4385299304 cites W3171752412 @default.
• W4385299304 cites W3172192134 @default.
• W4385299304 cites W3175436048 @default.
• W4385299304 cites W3179128182 @default.
• W4385299304 cites W3188896950 @default.
• W4385299304 cites W3197781442 @default.
• W4385299304 cites W3209558417 @default.
• W4385299304 cites W3210807621 @default.

• next