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- W4385307675 abstract "2,5-Furandicarboxylic acid (FDCA) is an important monomer for manufacturing biobased plastics. Biocatalysis has been recognized as a sustainable tool in organic synthesis. To date, the efficiencies of most biocatalytic processes toward FDCA remain low. So, it is highly desired to develop efficient processes. In this work, a biocatalytic route toward FDCA was developed by integrating a cell-free extract of galactose oxidase variant M3–5 with a whole-cell biocatalyst harboring NAD+-dependent vanillin dehydrogenases and NADH oxidase, starting from 5-hydroxymethylfurfural. FDCA was produced in a concurrent mode with >90% yields within 36 h at 20 mM substrate concentration. In addition, biocatalytic synthesis of FDCA was performed on a preparative scale, with 78% isolated yield. The present work may lay the foundation for sustainable production of FDCA." @default.
- W4385307675 created "2023-07-28" @default.
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- W4385307675 date "2023-07-27" @default.
- W4385307675 modified "2023-09-26" @default.
- W4385307675 title "Concurrent Biocatalytic Oxidation of 5-Hydroxymethylfurfural into 2,5-Furandicarboxylic Acid by Merging Galactose Oxidase with Whole Cells" @default.
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- W4385307675 doi "https://doi.org/10.3390/pr11082261" @default.
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