Matches in SemOpenAlex for { <https://semopenalex.org/work/W4385331314> ?p ?o ?g. }
- W4385331314 abstract "Lack of gene-function analyses tools limits studying the biology of Hanseniaspora uvarum, one of the most abundant yeasts on grapes and in must. We investigated a rapid PCR-based gene targeting approach for one-step gene replacement in this diploid yeast. To this end, we generated and validated two synthetic antibiotic resistance genes, pFA-hygXL and pFA-clnXL, providing resistance against hygromycin and nourseothricin, respectively, for use with H. uvarum. Addition of short flanking-homology regions of 56-80 bp to these selection markers via PCR was sufficient to promote gene targeting. We report here the deletion of the H. uvarum LEU2 and LYS2 genes with these marker genes via two rounds of consecutive transformations, each resulting in the generation of auxotrophic strains (leu2/leu2; lys2/lys2). The hereby constructed leucine auxotrophic leu2/leu2 strain was subsequently complemented in a targeted manner, thereby further validating this approach. PCR-based gene targeting in H. uvarum was less efficient than in Saccharomyces cerevisiae. However, this approach, combined with the availability of two marker genes, provides essential tools for directed gene manipulations in H. uvarum." @default.
- W4385331314 created "2023-07-29" @default.
- W4385331314 creator A5029884951 @default.
- W4385331314 creator A5054274305 @default.
- W4385331314 creator A5059343247 @default.
- W4385331314 creator A5075895444 @default.
- W4385331314 creator A5079782893 @default.
- W4385331314 creator A5087070178 @default.
- W4385331314 date "2023-01-01" @default.
- W4385331314 modified "2023-10-16" @default.
- W4385331314 title "PCR-based gene targeting in <i>Hanseniaspora uvarum</i>" @default.
- W4385331314 cites W1985484513 @default.
- W4385331314 cites W1985737988 @default.
- W4385331314 cites W1987342892 @default.
- W4385331314 cites W1990265320 @default.
- W4385331314 cites W2005092344 @default.
- W4385331314 cites W2009604447 @default.
- W4385331314 cites W2012575511 @default.
- W4385331314 cites W2018820266 @default.
- W4385331314 cites W2026969336 @default.
- W4385331314 cites W2038591876 @default.
- W4385331314 cites W2044545157 @default.
- W4385331314 cites W2071320894 @default.
- W4385331314 cites W2074984973 @default.
- W4385331314 cites W2080408951 @default.
- W4385331314 cites W2097703184 @default.
- W4385331314 cites W2100057937 @default.
- W4385331314 cites W2111997766 @default.
- W4385331314 cites W2114941304 @default.
- W4385331314 cites W2115727633 @default.
- W4385331314 cites W2119179068 @default.
- W4385331314 cites W2166188249 @default.
- W4385331314 cites W2169333620 @default.
- W4385331314 cites W2213454576 @default.
- W4385331314 cites W2346012907 @default.
- W4385331314 cites W2534173952 @default.
- W4385331314 cites W2752347988 @default.
- W4385331314 cites W2783818682 @default.
- W4385331314 cites W2883805256 @default.
- W4385331314 cites W2883931272 @default.
- W4385331314 cites W2911336472 @default.
- W4385331314 cites W2929740617 @default.
- W4385331314 cites W2972860576 @default.
- W4385331314 cites W2997952351 @default.
- W4385331314 cites W3000186995 @default.
- W4385331314 cites W3002383611 @default.
- W4385331314 cites W3036009728 @default.
- W4385331314 cites W3087186636 @default.
- W4385331314 cites W3131315941 @default.
- W4385331314 cites W3134293046 @default.
- W4385331314 cites W4211245981 @default.
- W4385331314 cites W4312071220 @default.
- W4385331314 cites W4317107917 @default.
- W4385331314 cites W4321749579 @default.
- W4385331314 doi "https://doi.org/10.1093/femsyr/foad034" @default.
- W4385331314 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37500280" @default.
- W4385331314 hasPublicationYear "2023" @default.
- W4385331314 type Work @default.
- W4385331314 citedByCount "1" @default.
- W4385331314 countsByYear W43853313142023 @default.
- W4385331314 crossrefType "journal-article" @default.
- W4385331314 hasAuthorship W4385331314A5029884951 @default.
- W4385331314 hasAuthorship W4385331314A5054274305 @default.
- W4385331314 hasAuthorship W4385331314A5059343247 @default.
- W4385331314 hasAuthorship W4385331314A5075895444 @default.
- W4385331314 hasAuthorship W4385331314A5079782893 @default.
- W4385331314 hasAuthorship W4385331314A5087070178 @default.
- W4385331314 hasConcept C104317684 @default.
- W4385331314 hasConcept C111829913 @default.
- W4385331314 hasConcept C165525559 @default.
- W4385331314 hasConcept C20950039 @default.
- W4385331314 hasConcept C22744801 @default.
- W4385331314 hasConcept C2780058085 @default.
- W4385331314 hasConcept C54355233 @default.
- W4385331314 hasConcept C547475151 @default.
- W4385331314 hasConcept C70721500 @default.
- W4385331314 hasConcept C86803240 @default.
- W4385331314 hasConceptScore W4385331314C104317684 @default.
- W4385331314 hasConceptScore W4385331314C111829913 @default.
- W4385331314 hasConceptScore W4385331314C165525559 @default.
- W4385331314 hasConceptScore W4385331314C20950039 @default.
- W4385331314 hasConceptScore W4385331314C22744801 @default.
- W4385331314 hasConceptScore W4385331314C2780058085 @default.
- W4385331314 hasConceptScore W4385331314C54355233 @default.
- W4385331314 hasConceptScore W4385331314C547475151 @default.
- W4385331314 hasConceptScore W4385331314C70721500 @default.
- W4385331314 hasConceptScore W4385331314C86803240 @default.
- W4385331314 hasFunder F4320334704 @default.
- W4385331314 hasLocation W43853313141 @default.
- W4385331314 hasLocation W43853313142 @default.
- W4385331314 hasOpenAccess W4385331314 @default.
- W4385331314 hasPrimaryLocation W43853313141 @default.
- W4385331314 hasRelatedWork W1991471459 @default.
- W4385331314 hasRelatedWork W2029633625 @default.
- W4385331314 hasRelatedWork W2034625591 @default.
- W4385331314 hasRelatedWork W2040571417 @default.
- W4385331314 hasRelatedWork W2045313050 @default.
- W4385331314 hasRelatedWork W2050573380 @default.
- W4385331314 hasRelatedWork W2074557265 @default.
- W4385331314 hasRelatedWork W2076127294 @default.