Matches in SemOpenAlex for { <https://semopenalex.org/work/W4385350524> ?p ?o ?g. }
Showing items 1 to 96 of
96
with 100 items per page.
- W4385350524 abstract "ABSTRACT Rubella virus remains a global health threat. Rubella infections during pregnancy can cause serious congenital pathology and no antiviral treatments are available. Rubella virus encodes a nonstructural polyprotein with RNA polymerase, methyltransferase, and papain-like cysteine protease activities, along with a putative macro domain of unknown function. Macro domains bind ADP-ribose adducts, a post-translational modification that plays a key role in host-virus conflicts. Some macro domains can also remove the mono-ADP-ribose adduct or degrade poly-ADP-ribose chains. Here, we report high-resolution crystal structures of the macro domain from rubella virus nonstructural protein p150, with and without ADP-ribose bound. The overall fold is most similar to macroD-type macro domains from various nonviral species. The specific composition and structure of the residues that poised for catalysis or coordinate ADP-ribose in the rubella virus macro domain are most similar to those of macro domains from alphaviruses. Isothermal calorimetry and enzymatic assays show that the rubella virus macro domain binds ADP-ribose in solution and has mono-ADP-ribosylhydrolase (de-MARylation) activity. IMPORTANCE Our work demonstrates that, like alpha- and coronaviruses, rubiviruses encode a mono-ADP-ribosylhydrolase with a structurally conserved macro domain fold to counteract MARylation by PARPs in the host innate immune response. Our structural data will guide future efforts to develop novel antiviral therapeutics against rubella or infections with related viruses." @default.
- W4385350524 created "2023-07-29" @default.
- W4385350524 creator A5046067654 @default.
- W4385350524 creator A5059646505 @default.
- W4385350524 creator A5090416149 @default.
- W4385350524 date "2023-07-28" @default.
- W4385350524 modified "2023-09-24" @default.
- W4385350524 title "Crystal structure and biochemical activity of the macro domain from rubella virus p150" @default.
- W4385350524 cites W1484931598 @default.
- W4385350524 cites W1973893673 @default.
- W4385350524 cites W1975272351 @default.
- W4385350524 cites W1980421520 @default.
- W4385350524 cites W1982285205 @default.
- W4385350524 cites W1996319727 @default.
- W4385350524 cites W2019184993 @default.
- W4385350524 cites W2030517878 @default.
- W4385350524 cites W2033554372 @default.
- W4385350524 cites W2040091616 @default.
- W4385350524 cites W2053716870 @default.
- W4385350524 cites W2074473216 @default.
- W4385350524 cites W2090117197 @default.
- W4385350524 cites W2097347921 @default.
- W4385350524 cites W2108921801 @default.
- W4385350524 cites W2133706960 @default.
- W4385350524 cites W2143660875 @default.
- W4385350524 cites W2144081223 @default.
- W4385350524 cites W2144635280 @default.
- W4385350524 cites W2149301799 @default.
- W4385350524 cites W2157509471 @default.
- W4385350524 cites W2159857626 @default.
- W4385350524 cites W2162923005 @default.
- W4385350524 cites W2169274959 @default.
- W4385350524 cites W2170370930 @default.
- W4385350524 cites W2180229411 @default.
- W4385350524 cites W2217204947 @default.
- W4385350524 cites W2232780020 @default.
- W4385350524 cites W2415408105 @default.
- W4385350524 cites W2503176400 @default.
- W4385350524 cites W2584528688 @default.
- W4385350524 cites W2816158287 @default.
- W4385350524 cites W2915543899 @default.
- W4385350524 cites W3043662389 @default.
- W4385350524 cites W3082858713 @default.
- W4385350524 cites W3092511958 @default.
- W4385350524 cites W3095507416 @default.
- W4385350524 cites W3105944734 @default.
- W4385350524 cites W3138723542 @default.
- W4385350524 cites W4220973428 @default.
- W4385350524 cites W4281954970 @default.
- W4385350524 cites W4285006034 @default.
- W4385350524 cites W4313484931 @default.
- W4385350524 doi "https://doi.org/10.1101/2023.07.28.550942" @default.
- W4385350524 hasPublicationYear "2023" @default.
- W4385350524 type Work @default.
- W4385350524 citedByCount "0" @default.
- W4385350524 crossrefType "posted-content" @default.
- W4385350524 hasAuthorship W4385350524A5046067654 @default.
- W4385350524 hasAuthorship W4385350524A5059646505 @default.
- W4385350524 hasAuthorship W4385350524A5090416149 @default.
- W4385350524 hasBestOaLocation W43853505241 @default.
- W4385350524 hasConcept C159047783 @default.
- W4385350524 hasConcept C181199279 @default.
- W4385350524 hasConcept C22070199 @default.
- W4385350524 hasConcept C2776438120 @default.
- W4385350524 hasConcept C2778097271 @default.
- W4385350524 hasConcept C2778814567 @default.
- W4385350524 hasConcept C55493867 @default.
- W4385350524 hasConcept C70721500 @default.
- W4385350524 hasConcept C82381507 @default.
- W4385350524 hasConcept C86803240 @default.
- W4385350524 hasConceptScore W4385350524C159047783 @default.
- W4385350524 hasConceptScore W4385350524C181199279 @default.
- W4385350524 hasConceptScore W4385350524C22070199 @default.
- W4385350524 hasConceptScore W4385350524C2776438120 @default.
- W4385350524 hasConceptScore W4385350524C2778097271 @default.
- W4385350524 hasConceptScore W4385350524C2778814567 @default.
- W4385350524 hasConceptScore W4385350524C55493867 @default.
- W4385350524 hasConceptScore W4385350524C70721500 @default.
- W4385350524 hasConceptScore W4385350524C82381507 @default.
- W4385350524 hasConceptScore W4385350524C86803240 @default.
- W4385350524 hasLocation W43853505241 @default.
- W4385350524 hasOpenAccess W4385350524 @default.
- W4385350524 hasPrimaryLocation W43853505241 @default.
- W4385350524 hasRelatedWork W1976391647 @default.
- W4385350524 hasRelatedWork W2012442783 @default.
- W4385350524 hasRelatedWork W2058116243 @default.
- W4385350524 hasRelatedWork W2059993433 @default.
- W4385350524 hasRelatedWork W2077408632 @default.
- W4385350524 hasRelatedWork W2142945422 @default.
- W4385350524 hasRelatedWork W237480860 @default.
- W4385350524 hasRelatedWork W2398032464 @default.
- W4385350524 hasRelatedWork W4240190450 @default.
- W4385350524 hasRelatedWork W68289550 @default.
- W4385350524 isParatext "false" @default.
- W4385350524 isRetracted "false" @default.
- W4385350524 workType "article" @default.