Matches in SemOpenAlex for { <https://semopenalex.org/work/W4385362308> ?p ?o ?g. }
- W4385362308 abstract "Metagenomics is a powerful tool to study marine microbial communities. However, obtaining high-quality environmental DNA suitable for downstream sequencing applications is a challenging task. The quality and quantity of isolated DNA heavily depend on the choice of purification procedure and the type of sample. Selection of an appropriate DNA isolation method for a new type of material often entails a lengthy trial and error process. Further, each DNA purification approach introduces biases and thus affects the composition of the studied community. To account for these problems and biases, we systematically investigated efficiency of DNA purification from three types of samples (water, sea sediment, and digestive tract of a model invertebrate Magallana gigas) with eight commercially available microbial DNA isolation kits. For each kit-sample combination we measured the quantity of purified DNA, extent of DNA fragmentation, the presence of PCR-inhibiting contaminants, admixture of eukaryotic DNA, alpha-diversity, and reproducibility of the resulting community composition based on 16S rRNA amplicons sequencing. Additionally, we determined a kitome, e.g., a set of contaminating taxa inherent for each type of purification kit used. The resulting matrix of evaluated parameters allows one to select the best DNA purification procedure for a given type of sample." @default.
- W4385362308 created "2023-07-29" @default.
- W4385362308 creator A5000863514 @default.
- W4385362308 creator A5006247345 @default.
- W4385362308 creator A5013140526 @default.
- W4385362308 creator A5046313743 @default.
- W4385362308 creator A5056880626 @default.
- W4385362308 creator A5064514026 @default.
- W4385362308 creator A5067832296 @default.
- W4385362308 creator A5073872536 @default.
- W4385362308 creator A5074229082 @default.
- W4385362308 creator A5076989154 @default.
- W4385362308 creator A5092564313 @default.
- W4385362308 date "2023-07-26" @default.
- W4385362308 modified "2023-09-26" @default.
- W4385362308 title "Benchmarking DNA Isolation Methods for Marine Metagenomics Studies" @default.
- W4385362308 cites W1523894467 @default.
- W4385362308 cites W1546091139 @default.
- W4385362308 cites W1717085993 @default.
- W4385362308 cites W1969029468 @default.
- W4385362308 cites W1973207608 @default.
- W4385362308 cites W1979577441 @default.
- W4385362308 cites W1988925586 @default.
- W4385362308 cites W1996738992 @default.
- W4385362308 cites W1999519761 @default.
- W4385362308 cites W2001408278 @default.
- W4385362308 cites W2024848067 @default.
- W4385362308 cites W2028460786 @default.
- W4385362308 cites W2034285706 @default.
- W4385362308 cites W2038871965 @default.
- W4385362308 cites W2039696994 @default.
- W4385362308 cites W2056279562 @default.
- W4385362308 cites W2059124668 @default.
- W4385362308 cites W2062994865 @default.
- W4385362308 cites W2073742361 @default.
- W4385362308 cites W2079397498 @default.
- W4385362308 cites W2087671769 @default.
- W4385362308 cites W2103296919 @default.
- W4385362308 cites W2110153690 @default.
- W4385362308 cites W2110501358 @default.
- W4385362308 cites W2120659830 @default.
- W4385362308 cites W2123506747 @default.
- W4385362308 cites W2126809954 @default.
- W4385362308 cites W2127175247 @default.
- W4385362308 cites W2127303481 @default.
- W4385362308 cites W2140319224 @default.
- W4385362308 cites W2149551170 @default.
- W4385362308 cites W2155299582 @default.
- W4385362308 cites W2159008215 @default.
- W4385362308 cites W2179507357 @default.
- W4385362308 cites W2230907825 @default.
- W4385362308 cites W2297571558 @default.
- W4385362308 cites W2401913426 @default.
- W4385362308 cites W2521004200 @default.
- W4385362308 cites W2556929151 @default.
- W4385362308 cites W2562916609 @default.
- W4385362308 cites W2579533542 @default.
- W4385362308 cites W2599498096 @default.
- W4385362308 cites W2623639676 @default.
- W4385362308 cites W2731562184 @default.
- W4385362308 cites W2745006471 @default.
- W4385362308 cites W2753961085 @default.
- W4385362308 cites W2754579667 @default.
- W4385362308 cites W2765432080 @default.
- W4385362308 cites W2766358903 @default.
- W4385362308 cites W2766645990 @default.
- W4385362308 cites W2776272136 @default.
- W4385362308 cites W2794904294 @default.
- W4385362308 cites W2796632532 @default.
- W4385362308 cites W2803457956 @default.
- W4385362308 cites W2885601766 @default.
- W4385362308 cites W2898868157 @default.
- W4385362308 cites W2900872097 @default.
- W4385362308 cites W2911798461 @default.
- W4385362308 cites W2917576966 @default.
- W4385362308 cites W2918015646 @default.
- W4385362308 cites W2949289629 @default.
- W4385362308 cites W2949372008 @default.
- W4385362308 cites W2949820666 @default.
- W4385362308 cites W2950993016 @default.
- W4385362308 cites W3004295003 @default.
- W4385362308 cites W3024176138 @default.
- W4385362308 cites W3047575572 @default.
- W4385362308 cites W3087330812 @default.
- W4385362308 cites W3095159768 @default.
- W4385362308 cites W3124177967 @default.
- W4385362308 cites W3130750627 @default.
- W4385362308 cites W3132450255 @default.
- W4385362308 cites W3136251000 @default.
- W4385362308 cites W3138273830 @default.
- W4385362308 cites W3148457808 @default.
- W4385362308 cites W3150323554 @default.
- W4385362308 cites W3160729269 @default.
- W4385362308 cites W3166722205 @default.
- W4385362308 cites W3204881459 @default.
- W4385362308 cites W3206528673 @default.
- W4385362308 cites W3206548644 @default.
- W4385362308 cites W3214432604 @default.
- W4385362308 cites W4210952527 @default.
- W4385362308 cites W4280528484 @default.