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• W4385363941 abstract "<i>Pterocarpus macrocarpus</i>, a perennial, woody tree, is considered as a commercially valuable species with various applications in furniture making, building materials, drug development, and dyeing. The genus <i>Pterocarpus</i> comprises 66 species growing throughout the tropics, with <i>P. macrocarpus</i> and <i>P. indicus</i> being morphologically closely related, leading to difficulties in distinguishing them via anatomical features. The calcium-dependent protein kinase (CPK) gene is a Ser/Thr protein kinase existing in plants, whose Ca²⁺ sensing and kinase activities play a significant role in plant growth, development and response to various stresses. However, current molecular methods such as DNA barcoding and phylogenetic analysis are time-consuming and labour-intensive. In the present study, a rapid and reliable real-time PCR method for wood identification of this species was established. Through target regions selection, primer/probe design and testing, method validation, specificity and sensitivity analysis, the most efficient real-time PCR approach taking partial CPK gene region as the target, was finally built up. It has also been proven to be highly specific and sensitive with a detection limit near 1.8 × 10^-2 ng/μL. This study provides a useful tool for wood species discrimination for the proper utilization of this valuable timber, which will certainly benefit the wood industry towards a better and reasonable circumstance." @default.
• W4385363941 created "2023-07-29" @default.
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• W4385363941 date "2023-07-17" @default.
• W4385363941 modified "2023-09-23" @default.
• W4385363941 title "Rapid Species Discrimination of High Value Hardwood -- &lt;i&gt;Pterocarpus macrocarpus&lt;/i&gt; via Real-Time PCR" @default.
• W4385363941 doi "https://doi.org/10.11648/j.jps.20231104.12" @default.
• W4385363941 hasPublicationYear "2023" @default.
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