Matches in SemOpenAlex for { <https://semopenalex.org/work/W4385382021> ?p ?o ?g. }
Showing items 1 to 100 of
100
with 100 items per page.
- W4385382021 endingPage "832" @default.
- W4385382021 startingPage "823" @default.
- W4385382021 abstract "Choosing fusion tags to enhance the recombinant protein levels in the cytoplasm of Bacillus subtilis has been limited. Our previous study demonstrated that His-tag at the N-terminus could increase the expression levels of the low-expression gene egfp, while significantly reducing the high-expression genes gfp+ and bgaB in the cytoplasm of B. subtilis. In this study, we aimed to prove the potential of a fusion tag, the combination of the N-terminal domain of B. subtilis lysyl tRNA synthetase (LysSN) and His-tag with varying numbers of histidine (6xHis, 8xHis, 10xHis) by investigating their effects on the expression levels of egfp, gfp+ and bgaB in B. subtilis. For the low-expression gene, LysSN-xHis-tag could enhance the fluorescent intensity of EGFP 23.5 times higher than EGFP without a fusion tag, and 1.5 times higher than that fused with only His-tag. For high-expression genes, the expression level of BgaB and GFP+ was 2.9 and 12.5 times higher than that of His-tag, respectively. The number of histidines in LysSN-xHis-tag did not influence the expression levels of the high-expression genes but affected the expression levels of the low-expression gene." @default.
- W4385382021 created "2023-07-30" @default.
- W4385382021 creator A5007439601 @default.
- W4385382021 creator A5008389029 @default.
- W4385382021 creator A5038118035 @default.
- W4385382021 creator A5041481111 @default.
- W4385382021 creator A5070181852 @default.
- W4385382021 date "2023-07-29" @default.
- W4385382021 modified "2023-10-17" @default.
- W4385382021 title "N‐terminal LysSN‐His‐tag improves the production of intracellular recombinant protein in <i>Bacillus subtilis</i>" @default.
- W4385382021 cites W181294179 @default.
- W4385382021 cites W1831169804 @default.
- W4385382021 cites W2004369836 @default.
- W4385382021 cites W2012630600 @default.
- W4385382021 cites W2037701570 @default.
- W4385382021 cites W2040953453 @default.
- W4385382021 cites W2068215206 @default.
- W4385382021 cites W2079466113 @default.
- W4385382021 cites W2080434522 @default.
- W4385382021 cites W2097152121 @default.
- W4385382021 cites W2104756056 @default.
- W4385382021 cites W2503709851 @default.
- W4385382021 cites W2610911866 @default.
- W4385382021 cites W2620923907 @default.
- W4385382021 cites W2738984803 @default.
- W4385382021 cites W2776396270 @default.
- W4385382021 cites W2784650400 @default.
- W4385382021 cites W2884563437 @default.
- W4385382021 cites W2892377899 @default.
- W4385382021 cites W2907621117 @default.
- W4385382021 cites W2907661665 @default.
- W4385382021 cites W2913336594 @default.
- W4385382021 cites W2981150417 @default.
- W4385382021 cites W2999150714 @default.
- W4385382021 cites W3004233517 @default.
- W4385382021 cites W3081744280 @default.
- W4385382021 cites W3176189464 @default.
- W4385382021 cites W3195182848 @default.
- W4385382021 cites W3210430327 @default.
- W4385382021 cites W4286249415 @default.
- W4385382021 doi "https://doi.org/10.1002/cbf.3832" @default.
- W4385382021 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37515537" @default.
- W4385382021 hasPublicationYear "2023" @default.
- W4385382021 type Work @default.
- W4385382021 citedByCount "0" @default.
- W4385382021 crossrefType "journal-article" @default.
- W4385382021 hasAuthorship W4385382021A5007439601 @default.
- W4385382021 hasAuthorship W4385382021A5008389029 @default.
- W4385382021 hasAuthorship W4385382021A5038118035 @default.
- W4385382021 hasAuthorship W4385382021A5041481111 @default.
- W4385382021 hasAuthorship W4385382021A5070181852 @default.
- W4385382021 hasConcept C104317684 @default.
- W4385382021 hasConcept C111829193 @default.
- W4385382021 hasConcept C123894998 @default.
- W4385382021 hasConcept C142613039 @default.
- W4385382021 hasConcept C150194340 @default.
- W4385382021 hasConcept C153911025 @default.
- W4385382021 hasConcept C185592680 @default.
- W4385382021 hasConcept C190062978 @default.
- W4385382021 hasConcept C2777272437 @default.
- W4385382021 hasConcept C40767141 @default.
- W4385382021 hasConcept C523546767 @default.
- W4385382021 hasConcept C54355233 @default.
- W4385382021 hasConcept C55493867 @default.
- W4385382021 hasConcept C86803240 @default.
- W4385382021 hasConceptScore W4385382021C104317684 @default.
- W4385382021 hasConceptScore W4385382021C111829193 @default.
- W4385382021 hasConceptScore W4385382021C123894998 @default.
- W4385382021 hasConceptScore W4385382021C142613039 @default.
- W4385382021 hasConceptScore W4385382021C150194340 @default.
- W4385382021 hasConceptScore W4385382021C153911025 @default.
- W4385382021 hasConceptScore W4385382021C185592680 @default.
- W4385382021 hasConceptScore W4385382021C190062978 @default.
- W4385382021 hasConceptScore W4385382021C2777272437 @default.
- W4385382021 hasConceptScore W4385382021C40767141 @default.
- W4385382021 hasConceptScore W4385382021C523546767 @default.
- W4385382021 hasConceptScore W4385382021C54355233 @default.
- W4385382021 hasConceptScore W4385382021C55493867 @default.
- W4385382021 hasConceptScore W4385382021C86803240 @default.
- W4385382021 hasIssue "7" @default.
- W4385382021 hasLocation W43853820211 @default.
- W4385382021 hasLocation W43853820212 @default.
- W4385382021 hasOpenAccess W4385382021 @default.
- W4385382021 hasPrimaryLocation W43853820211 @default.
- W4385382021 hasRelatedWork W1548672655 @default.
- W4385382021 hasRelatedWork W2025879395 @default.
- W4385382021 hasRelatedWork W2350892013 @default.
- W4385382021 hasRelatedWork W2360861458 @default.
- W4385382021 hasRelatedWork W2373087578 @default.
- W4385382021 hasRelatedWork W2401437080 @default.
- W4385382021 hasRelatedWork W2973460871 @default.
- W4385382021 hasRelatedWork W3025683560 @default.
- W4385382021 hasRelatedWork W3041516390 @default.
- W4385382021 hasRelatedWork W4293061103 @default.
- W4385382021 hasVolume "41" @default.
- W4385382021 isParatext "false" @default.
- W4385382021 isRetracted "false" @default.
- W4385382021 workType "article" @default.