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- W4385387612 abstract "The introduction of calcium imaging has rendered cnidarians, such as Hydra vulgaris, valuable model organisms for investigating neuronal activity and behavior. Here, we present a comprehensive protocol to image and manipulate neuronal activity and behavior of Hydra. We describe steps for wide-field imaging and two-photon simulation and ablation of neurons. We then detail imaging behavior and post-ablation analysis. We address challenges that may arise during the preparation and execution of the experiments." @default.
- W4385387612 created "2023-07-30" @default.
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- W4385387612 date "2023-09-01" @default.
- W4385387612 modified "2023-09-30" @default.
- W4385387612 title "Two-photon manipulation of neuronal activity and behavior in Hydra vulgaris" @default.
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- W4385387612 doi "https://doi.org/10.1016/j.xpro.2023.102453" @default.
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