FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4385387966> ?p ?o ?g. }

• W4385387966 abstract "Posttranslational regulation of cardiac NaV1.5 channels is critical in modulating channel expression and function, yet their regulation by phosphorylation of accessory proteins has gone largely unexplored. Using phosphoproteomic analysis of NaV channel complexes from adult mouse left ventricles, we identified nine phosphorylation sites on intracellular fibroblast growth factor 13 (iFGF13). To explore the potential roles of these phosphosites in regulating cardiac NaV currents, we abolished expression of iFGF13 in neonatal and adult mouse ventricular myocytes and rescued it with wild-type (WT), phosphosilent, or phosphomimetic iFGF13-VY. While the increased rate of closed-state inactivation of NaV channels induced by Fgf13 knockout in adult cardiomyocytes was completely restored by adenoviral-mediated expression of WT iFGF13-VY, only partial rescue was observed in neonatal cardiomyocytes after knockdown. The knockdown of iFGF13 in neonatal ventricular myocytes also shifted the voltage dependence of channel activation toward hyperpolarized potentials, a shift that was not reversed by WT iFGF13-VY expression. Additionally, we found that iFGF13-VY is the predominant isoform in adult ventricular myocytes, whereas both iFGF13-VY and iFGF13-S are expressed comparably in neonatal ventricular myocytes. Similar to WT iFGF13-VY, each of the iFGF13-VY phosphomutants studied restored NaV channel inactivation properties in both models. Lastly, Fgf13 knockout also increased the late Na+ current in adult cardiomyocytes, and this effect was restored with expression of WT and phosphosilent iFGF13-VY. Together, our results demonstrate that iFGF13 is highly phosphorylated and displays differential isoform expression in neonatal and adult ventricular myocytes. While we found no roles for iFGF13 phosphorylation, our results demonstrate differential effects of iFGF13 on neonatal and adult mouse ventricular NaV channels." @default.
• W4385387966 created "2023-07-30" @default.
• W4385387966 creator A5010129022 @default.
• W4385387966 creator A5013302750 @default.
• W4385387966 creator A5014705357 @default.
• W4385387966 creator A5021632525 @default.
• W4385387966 creator A5024769314 @default.
• W4385387966 creator A5024889977 @default.
• W4385387966 creator A5029528576 @default.
• W4385387966 creator A5044189301 @default.
• W4385387966 creator A5057102063 @default.
• W4385387966 creator A5086677370 @default.
• W4385387966 creator A5087985631 @default.
• W4385387966 date "2023-07-27" @default.
• W4385387966 modified "2023-10-15" @default.
• W4385387966 title "Determinants of iFGF13-mediated regulation of myocardial voltage-gated sodium (NaV) channels in mouse" @default.
• W4385387966 cites W1528710182 @default.
• W4385387966 cites W1551685734 @default.
• W4385387966 cites W1689714005 @default.
• W4385387966 cites W1913287761 @default.
• W4385387966 cites W1966093407 @default.
• W4385387966 cites W1973592885 @default.
• W4385387966 cites W1981619826 @default.
• W4385387966 cites W2000763279 @default.
• W4385387966 cites W2008093629 @default.
• W4385387966 cites W2026945534 @default.
• W4385387966 cites W2037199116 @default.
• W4385387966 cites W2037903288 @default.
• W4385387966 cites W2047253203 @default.
• W4385387966 cites W2061321767 @default.
• W4385387966 cites W2068538433 @default.
• W4385387966 cites W2070249204 @default.
• W4385387966 cites W2088645977 @default.
• W4385387966 cites W2090654893 @default.
• W4385387966 cites W2095345152 @default.
• W4385387966 cites W2121234157 @default.
• W4385387966 cites W2135078307 @default.
• W4385387966 cites W2143296458 @default.
• W4385387966 cites W2148626683 @default.
• W4385387966 cites W2152501972 @default.
• W4385387966 cites W2158302225 @default.
• W4385387966 cites W2293181095 @default.
• W4385387966 cites W2412690797 @default.
• W4385387966 cites W2528536193 @default.
• W4385387966 cites W2575931164 @default.
• W4385387966 cites W2587120326 @default.
• W4385387966 cites W2614597148 @default.
• W4385387966 cites W2743702028 @default.
• W4385387966 cites W2751216183 @default.
• W4385387966 cites W2807803570 @default.
• W4385387966 cites W2928930495 @default.
• W4385387966 cites W2996435836 @default.
• W4385387966 cites W3037224757 @default.
• W4385387966 cites W3081808591 @default.
• W4385387966 cites W3120693979 @default.
• W4385387966 cites W3126154915 @default.
• W4385387966 cites W4280638529 @default.
• W4385387966 cites W4286493299 @default.
• W4385387966 cites W4328051929 @default.
• W4385387966 doi "https://doi.org/10.1085/jgp.202213293" @default.
• W4385387966 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37516919" @default.
• W4385387966 hasPublicationYear "2023" @default.
• W4385387966 type Work @default.
• W4385387966 citedByCount "0" @default.
• W4385387966 crossrefType "journal-article" @default.
• W4385387966 hasAuthorship W4385387966A5010129022 @default.
• W4385387966 hasAuthorship W4385387966A5013302750 @default.
• W4385387966 hasAuthorship W4385387966A5014705357 @default.
• W4385387966 hasAuthorship W4385387966A5021632525 @default.
• W4385387966 hasAuthorship W4385387966A5024769314 @default.
• W4385387966 hasAuthorship W4385387966A5024889977 @default.
• W4385387966 hasAuthorship W4385387966A5029528576 @default.
• W4385387966 hasAuthorship W4385387966A5044189301 @default.
• W4385387966 hasAuthorship W4385387966A5057102063 @default.
• W4385387966 hasAuthorship W4385387966A5086677370 @default.
• W4385387966 hasAuthorship W4385387966A5087985631 @default.
• W4385387966 hasConcept C104317684 @default.
• W4385387966 hasConcept C11960822 @default.
• W4385387966 hasConcept C126322002 @default.
• W4385387966 hasConcept C134018914 @default.
• W4385387966 hasConcept C170493617 @default.
• W4385387966 hasConcept C173396325 @default.
• W4385387966 hasConcept C178790620 @default.
• W4385387966 hasConcept C182704531 @default.
• W4385387966 hasConcept C185592680 @default.
• W4385387966 hasConcept C207200792 @default.
• W4385387966 hasConcept C2776250582 @default.
• W4385387966 hasConcept C50952357 @default.
• W4385387966 hasConcept C53345823 @default.
• W4385387966 hasConcept C537181965 @default.
• W4385387966 hasConcept C54355233 @default.
• W4385387966 hasConcept C55493867 @default.
• W4385387966 hasConcept C71924100 @default.
• W4385387966 hasConcept C79879829 @default.
• W4385387966 hasConcept C81885089 @default.
• W4385387966 hasConcept C86803240 @default.
• W4385387966 hasConcept C95444343 @default.
• W4385387966 hasConceptScore W4385387966C104317684 @default.
• W4385387966 hasConceptScore W4385387966C11960822 @default.
• W4385387966 hasConceptScore W4385387966C126322002 @default.

• next