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• W4385420384 endingPage "809" @default.
• W4385420384 startingPage "809" @default.
• W4385420384 abstract "Fusarium basal rot disease (FBR) is a destructive threat to onion crops around the globe. It causes seedlings’ death, development disruption, and pre- and post-harvest bulb infection and rotting, with a concern for toxin infestation. It is an emerging disease in Israel, with new reports from farms nationwide. Recently, we reported on a full-season pot experiment to protect two leading commercial cultivars against FBR chemically. Here, we present new real-time qPCR molecular tracking of the pathogens inside the host plant and compare the infection levels to a deep analysis of the impacts of this experiment’s treatments on plant growth and health indexes. The new findings reveal variations within each treatment’s effectiveness regarding sprout development and bulb ripening stages. For instance, in the yellow Orlando cv., high protection was obtained with Azoxystrobin + Tebuconazole (Az-Te) in sprouts against F. oxysporum f. sp. cepae and with Fludioxonil + Sedaxen in mature plants against Fusarium acutatum. Thus, combining these fungicides may protect plants throughout their lifecycle. Also, Prochloraz at low dose was highly efficient in the Orlando cv. Still, to shield red Noam cv. plants from both pathogens, increasing this fungicide concentration towards the season-ending should be preferred. The qPCR tracking showed that all chemical treatments tested could reduce infection from pathogens by 80–90%, even with compounds such as Az-Te that were less effective. This implies that the pesticide was effective but probably phytotoxic to the plants, and thus, lower dosages must be considered. The molecular-based analysis discloses the high infection ability of F. oxysporum f. sp. cepae compared to F. acutatum in both cultivars. It also indicates an antagonism between those species in the Orlando cv. and synergism in the Noam cv. The current work reveals weak and strong points in chemical FBR protection and offers new ways to improve its application. The qPCR-based method enables us to closely monitor the pathogenesis and efficacy of chemical-preventing treatments and optimize crop-protection protocols." @default.
• W4385420384 created "2023-08-01" @default.
• W4385420384 creator A5072157767 @default.
• W4385420384 creator A5074514981 @default.
• W4385420384 date "2023-07-30" @default.
• W4385420384 modified "2023-09-30" @default.
• W4385420384 title "Molecular Real-Time PCR Monitoring of Onion Fusarium Basal Rot Chemical Control" @default.
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• W4385420384 doi "https://doi.org/10.3390/jof9080809" @default.
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