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• W4385422013 endingPage "12166" @default.
• W4385422013 startingPage "12166" @default.
• W4385422013 abstract "Exosomes are key mediators of intercellular communication. They are secreted by most cells and contain a cargo of protein-coding genes, long noncoding RNAs (lncRNAs), and circular RNAs (circRNAs), which modulate recipient cell behavior. Herein, we collected blood samples from Holstein cows at days 30 (mid-lactation) and 250 (dry period) of pregnancy. Prolactin, follicle-stimulating hormone, luteinizing hormone, estrogen, and progesterone levels showed an obvious increase during D250. We then extracted exosomes from bovine blood samples and found that their sizes generally ranged from 100 to 200 nm. Further, Western blotting validated that they contained CD9, CD63, and TSG101, but not calnexin. Blood-derived exosomes significantly promoted the proliferation of mammary epithelial cells, particularly from D250. This change was accompanied by increased expression levels of proliferation marker proteins PCNA, cyclin D, and cyclin E, as detected by EdU assay, cell counting kit-8 assay, and flow cytometric cell cycle analysis. Moreover, we treated mammary epithelial cells with blood-derived exosomes that were isolated from the D30 and D250 periods. And RNA-seq of two groups of cells led to the identification of 839 differentially expressed genes that were significantly enriched in KEGG signaling pathways associated with apoptosis, cell cycle and proliferation. In bovine blood-derived exosomes, we found 12,747 protein-coding genes, 31,181 lncRNAs, 9374 transcripts of uncertain coding potential (TUCP) candidates, and 460 circRNAs, and 32 protein-coding genes, 806 lncRNAs, 515 TUCP candidates, and 45 circRNAs that were differentially expressed between the D30 and D250 groups. We selected six highly expressed and four differentially expressed circRNAs to verify their head-to-tail splicing using PCR and Sanger sequencing. To summarize, our findings improve our understanding of the key roles of blood-derived exosomes and the characterization of exosomal circRNAs in mammary gland development." @default.
• W4385422013 created "2023-08-01" @default.
• W4385422013 creator A5019256562 @default.
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• W4385422013 date "2023-07-29" @default.
• W4385422013 modified "2023-09-27" @default.
• W4385422013 title "The Characteristic Function of Blood-Derived Exosomes and Exosomal circRNAs Isolated from Dairy Cattle during the Dry Period and Mid-Lactation" @default.
• W4385422013 cites W1678597207 @default.
• W4385422013 cites W1762620449 @default.
• W4385422013 cites W1846631206 @default.
• W4385422013 cites W1866220599 @default.
• W4385422013 cites W1931166099 @default.
• W4385422013 cites W1967613870 @default.
• W4385422013 cites W1978283443 @default.
• W4385422013 cites W1991082489 @default.
• W4385422013 cites W1999574084 @default.
• W4385422013 cites W2011218704 @default.
• W4385422013 cites W2015601897 @default.
• W4385422013 cites W2030701238 @default.
• W4385422013 cites W2055313665 @default.
• W4385422013 cites W2058835062 @default.
• W4385422013 cites W2059597550 @default.
• W4385422013 cites W2061548680 @default.
• W4385422013 cites W2074611304 @default.
• W4385422013 cites W2075320162 @default.
• W4385422013 cites W2078894965 @default.
• W4385422013 cites W2100305481 @default.
• W4385422013 cites W2102278945 @default.
• W4385422013 cites W2104005232 @default.
• W4385422013 cites W2105106534 @default.
• W4385422013 cites W2107060225 @default.
• W4385422013 cites W2114104545 @default.
• W4385422013 cites W2121725913 @default.
• W4385422013 cites W2130410032 @default.
• W4385422013 cites W2130634390 @default.
• W4385422013 cites W2136601868 @default.
• W4385422013 cites W2141642267 @default.
• W4385422013 cites W2147526198 @default.
• W4385422013 cites W2147806662 @default.
• W4385422013 cites W2158761186 @default.
• W4385422013 cites W2160333540 @default.
• W4385422013 cites W2170551349 @default.
• W4385422013 cites W2170852633 @default.
• W4385422013 cites W2178935826 @default.
• W4385422013 cites W2179438025 @default.
• W4385422013 cites W2256359187 @default.
• W4385422013 cites W2266238367 @default.
• W4385422013 cites W2325364720 @default.
• W4385422013 cites W2328929505 @default.
• W4385422013 cites W2330004628 @default.
• W4385422013 cites W2358858563 @default.
• W4385422013 cites W2510875395 @default.
• W4385422013 cites W2599812844 @default.
• W4385422013 cites W2615498501 @default.
• W4385422013 cites W2621169509 @default.
• W4385422013 cites W2756714961 @default.
• W4385422013 cites W2763176611 @default.
• W4385422013 cites W2767797402 @default.
• W4385422013 cites W2889170926 @default.
• W4385422013 cites W2901189092 @default.
• W4385422013 cites W2904941484 @default.
• W4385422013 cites W2947468234 @default.
• W4385422013 cites W2951912016 @default.
• W4385422013 cites W2959379447 @default.
• W4385422013 cites W2967929867 @default.
• W4385422013 cites W2989137800 @default.
• W4385422013 cites W3005104290 @default.
• W4385422013 cites W3010445736 @default.
• W4385422013 cites W3011894986 @default.
• W4385422013 cites W3017083206 @default.
• W4385422013 cites W3023230660 @default.
• W4385422013 cites W3028960763 @default.
• W4385422013 cites W3034353096 @default.
• W4385422013 cites W3039901296 @default.
• W4385422013 cites W3093702715 @default.
• W4385422013 cites W3109111679 @default.
• W4385422013 cites W3128227583 @default.
• W4385422013 cites W3154091431 @default.
• W4385422013 cites W3163570813 @default.
• W4385422013 cites W3179483189 @default.
• W4385422013 cites W3196172542 @default.
• W4385422013 cites W3208252812 @default.
• W4385422013 cites W4200018394 @default.
• W4385422013 cites W4220765739 @default.
• W4385422013 cites W4280638470 @default.
• W4385422013 cites W4283383567 @default.
• W4385422013 cites W4283460804 @default.
• W4385422013 cites W4283656103 @default.
• W4385422013 cites W4303648547 @default.
• W4385422013 cites W4307122622 @default.
• W4385422013 cites W4312094640 @default.
• W4385422013 doi "https://doi.org/10.3390/ijms241512166" @default.

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