FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4385446682> ?p ?o ?g. }

• W4385446682 abstract "Macrophage colony-stimulating factor 2 (MCSF-2) is an important cytokine that controls how cells of the monocyte/macrophage lineage proliferate, differentiate, and survive in vertebrates. Two isoforms of MCSF have been identified in fish, each exhibiting distinct gene organization and expression patterns. In this study, we investigated a goldfish MCSF-2 gene in terms of its immunomodulatory and functional properties.In this study, goldfish were acclimated for 3 weeks and sedated with TMS prior to handling. Two groups of fish were used for infection experiments, and tissues from healthy goldfish were collected for RNA isolation. cDNA synthesis was performed, and primers were designed based on transcriptome database sequences. Analysis of gfMCSF-2 sequences, including nucleotide and amino acid analysis, molecular mass prediction, and signal peptide prediction, was conducted. Real-time quantitative PCR (qPCR) was used to analyze gene expression levels, while goldfish head kidney leukocytes (HKLs) were isolated using standard protocols. The expression of gfMCSF-2 in activated HKLs was investigated, and recombinant goldfish MCSF-2 was expressed and purified. Western blot analysis, cell proliferation assays, and flow cytometric analysis of HKLs were performed. Gene expression analysis of transcription factors and pro-inflammatory cytokines in goldfish head kidney leukocytes exposed to rgMCSF-2 was conducted. Statistical analysis using one-way ANOVA and Dunnett's post hoc test was applied.We performed a comparative analysis of MCSF-1 and MCSF-2 at the protein and nucleotide levels using the Needleman-Wunsch algorithm. The results revealed significant differences between the two sequences, supporting the notion that they represent distinct genes rather than isoforms of the same gene. Sequence alignment demonstrated high sequence identity with MCSF-2 homologs from fish species, particularly C. carpio, which was supported by phylogenetic analysis. Expression analysis in various goldfish tissues demonstrated differential expression levels, with the spleen exhibiting the highest expression. In goldfish head kidney leukocytes, gfMCSF-2 expression was modulated by chemical stimuli and bacterial infection, with upregulation observed in response to lipopolysaccharide (LPS) and live Aeromonas hydrophila. Recombinant gfMCSF-2 (rgMCSF-2) was successfully expressed and purified, showing the ability to stimulate cell proliferation in HKLs. Flow cytometric analysis revealed that rgMCSF-2 induced differentiation of sorted leukocytes at a specific concentration. Moreover, rgMCSF-2 treatment upregulated TNFα and IL-1β mRNA levels and influenced the expression of transcription factors, such as MafB, GATA2, and cMyb, in a time-dependent manner.Collectively, by elucidating the effects of rgMCSF-2 on cell proliferation, differentiation, and the modulation of pro-inflammatory cytokines and transcription factors, our findings provided a comprehensive understanding of the potential mechanisms underlying gfMCSF-2-mediated immune regulation. These results contribute to the fundamental knowledge of MCSF-2 in teleosts and establish a foundation for further investigations on the role of gfMCSF-2 in fish immune responses." @default.
• W4385446682 created "2023-08-02" @default.
• W4385446682 creator A5010139342 @default.
• W4385446682 creator A5016741447 @default.
• W4385446682 creator A5025003111 @default.
• W4385446682 creator A5050656828 @default.
• W4385446682 creator A5085752724 @default.
• W4385446682 date "2023-08-01" @default.
• W4385446682 modified "2023-09-25" @default.
• W4385446682 title "Molecular characterization, gene expression and functional analysis of goldfish (Carassius auratus L.) macrophage colony stimulating factor 2" @default.
• W4385446682 cites W1524998078 @default.
• W4385446682 cites W1536628513 @default.
• W4385446682 cites W1927935170 @default.
• W4385446682 cites W1979540275 @default.
• W4385446682 cites W1981505729 @default.
• W4385446682 cites W1985140242 @default.
• W4385446682 cites W1989402989 @default.
• W4385446682 cites W1992433395 @default.
• W4385446682 cites W1998779768 @default.
• W4385446682 cites W1999420397 @default.
• W4385446682 cites W2001786687 @default.
• W4385446682 cites W2005464610 @default.
• W4385446682 cites W2015031635 @default.
• W4385446682 cites W2017020657 @default.
• W4385446682 cites W2018233370 @default.
• W4385446682 cites W2018846024 @default.
• W4385446682 cites W2026629236 @default.
• W4385446682 cites W2029002731 @default.
• W4385446682 cites W2031407638 @default.
• W4385446682 cites W2032410318 @default.
• W4385446682 cites W2036904886 @default.
• W4385446682 cites W2041870860 @default.
• W4385446682 cites W2042662552 @default.
• W4385446682 cites W2048990720 @default.
• W4385446682 cites W2056776025 @default.
• W4385446682 cites W2057885619 @default.
• W4385446682 cites W2059382129 @default.
• W4385446682 cites W2063632352 @default.
• W4385446682 cites W2067486077 @default.
• W4385446682 cites W2072462631 @default.
• W4385446682 cites W2079047148 @default.
• W4385446682 cites W2080596906 @default.
• W4385446682 cites W2095175490 @default.
• W4385446682 cites W2099521287 @default.
• W4385446682 cites W2106495713 @default.
• W4385446682 cites W2148467944 @default.
• W4385446682 cites W2155984519 @default.
• W4385446682 cites W2323113905 @default.
• W4385446682 cites W2558246554 @default.
• W4385446682 cites W2560831799 @default.
• W4385446682 cites W2891030997 @default.
• W4385446682 cites W2990603633 @default.
• W4385446682 cites W2995238336 @default.
• W4385446682 cites W3094633279 @default.
• W4385446682 cites W3101993969 @default.
• W4385446682 cites W3109636035 @default.
• W4385446682 doi "https://doi.org/10.3389/fimmu.2023.1235370" @default.
• W4385446682 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37593738" @default.
• W4385446682 hasPublicationYear "2023" @default.
• W4385446682 type Work @default.
• W4385446682 citedByCount "0" @default.
• W4385446682 crossrefType "journal-article" @default.
• W4385446682 hasAuthorship W4385446682A5010139342 @default.
• W4385446682 hasAuthorship W4385446682A5016741447 @default.
• W4385446682 hasAuthorship W4385446682A5025003111 @default.
• W4385446682 hasAuthorship W4385446682A5050656828 @default.
• W4385446682 hasAuthorship W4385446682A5085752724 @default.
• W4385446682 hasBestOaLocation W43854466821 @default.
• W4385446682 hasConcept C104317684 @default.
• W4385446682 hasConcept C105580179 @default.
• W4385446682 hasConcept C150194340 @default.
• W4385446682 hasConcept C153911025 @default.
• W4385446682 hasConcept C162317418 @default.
• W4385446682 hasConcept C187882448 @default.
• W4385446682 hasConcept C2776415932 @default.
• W4385446682 hasConcept C2781184567 @default.
• W4385446682 hasConcept C40767141 @default.
• W4385446682 hasConcept C53345823 @default.
• W4385446682 hasConcept C54355233 @default.
• W4385446682 hasConcept C86803240 @default.
• W4385446682 hasConceptScore W4385446682C104317684 @default.
• W4385446682 hasConceptScore W4385446682C105580179 @default.
• W4385446682 hasConceptScore W4385446682C150194340 @default.
• W4385446682 hasConceptScore W4385446682C153911025 @default.
• W4385446682 hasConceptScore W4385446682C162317418 @default.
• W4385446682 hasConceptScore W4385446682C187882448 @default.
• W4385446682 hasConceptScore W4385446682C2776415932 @default.
• W4385446682 hasConceptScore W4385446682C2781184567 @default.
• W4385446682 hasConceptScore W4385446682C40767141 @default.
• W4385446682 hasConceptScore W4385446682C53345823 @default.
• W4385446682 hasConceptScore W4385446682C54355233 @default.
• W4385446682 hasConceptScore W4385446682C86803240 @default.
• W4385446682 hasFunder F4320321001 @default.
• W4385446682 hasFunder F4320338464 @default.
• W4385446682 hasLocation W43854466821 @default.
• W4385446682 hasLocation W43854466822 @default.
• W4385446682 hasOpenAccess W4385446682 @default.
• W4385446682 hasPrimaryLocation W43854466821 @default.
• W4385446682 hasRelatedWork W2338137066 @default.
• W4385446682 hasRelatedWork W2339270935 @default.

• next