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- W4385451092 abstract "Abstract In this work, a self-assembly approach is proposed to bio-functionalize the fully microfluidic biosensors for catalyzing glucose based on the designed and fabricated microfluidic chip with suspending structure by micro-electro-mechanical system technology. Glucose oxidase is successfully immobilized on the surface of microelectrodes to build composite layers by the proposed self-assembly approach, with cysteamine applied as a functional linker. The biological activity and stability of glucose oxidase is optimized by the electrodeposition of chitosan membrane. Experiments results suggest that the fully packaged microfluidic biosensor integrated with self-assembled glucose oxidase can effectively detect the existence of glucose in sample solution. The detection range of glucose concentration is 0 ~ 10 mM. With SNR of 3, the detection limit is 51.2 µM and sensitivity is 23.47 ± 1.36 µA·cm − 2 ·mM − 1 within low concentration range of 0 ~ 0.1 mM, while the detection limit is 497 µM and the sensitivity is 10.63 ± 1.28 µA·cm − 2 ·mM − 1 among high concentration range of 0.1 ~ 10 mM. The proposed study provides a straightforward method to construct a microfluidic biosensor, which is promising in miniaturized clinical medical device." @default.
- W4385451092 created "2023-08-02" @default.
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- W4385451092 date "2023-08-01" @default.
- W4385451092 modified "2023-10-17" @default.
- W4385451092 title "Self-Assembly Method of Glucose Oxidase in a Fully Packaged Microfluidic Glucose Biosensor" @default.
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- W4385451092 doi "https://doi.org/10.21203/rs.3.rs-3205860/v1" @default.
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