Matches in SemOpenAlex for { <https://semopenalex.org/work/W4385481432> ?p ?o ?g. }
- W4385481432 endingPage "15846" @default.
- W4385481432 startingPage "15836" @default.
- W4385481432 abstract "Cryogenic electron microscopy can provide high-resolution reconstructions of macromolecules embedded in a thin layer of ice from which atomic models can be built de novo. However, the interaction between the ionizing electron beam and the sample results in beam-induced motion and image distortion, which limit the attainable resolutions. Sample charging is one contributing factor of beam-induced motions and image distortions, which is normally alleviated by including part of the supporting conducting film within the beam-exposed region. However, routine data collection schemes avoid strategies whereby the beam is not in contact with the supporting film, whose rationale is not fully understood. Here we characterize electrostatic charging of vitreous samples, both in imaging and in diffraction mode. We mitigate sample charging by depositing a single layer of conductive graphene on top of regular EM grids. We obtained high-resolution single-particle analysis (SPA) reconstructions at 2 Å when the electron beam only irradiates the middle of the hole on graphene-coated grids, using data collection schemes that previously failed to produce sub 3 Å reconstructions without the graphene layer. We also observe that the SPA data obtained with the graphene-coated grids exhibit a higher b factor and reduced particle movement compared to data obtained without the graphene layer. This mitigation of charging could have broad implications for various EM techniques, including SPA and cryotomography, and for the study of radiation damage and the development of future sample carriers. Furthermore, it may facilitate the exploration of more dose-efficient, scanning transmission EM based SPA techniques." @default.
- W4385481432 created "2023-08-03" @default.
- W4385481432 creator A5011638743 @default.
- W4385481432 creator A5013323925 @default.
- W4385481432 creator A5013888065 @default.
- W4385481432 creator A5019318669 @default.
- W4385481432 creator A5029504399 @default.
- W4385481432 creator A5037247357 @default.
- W4385481432 creator A5039549307 @default.
- W4385481432 creator A5065638633 @default.
- W4385481432 creator A5077999397 @default.
- W4385481432 creator A5083082662 @default.
- W4385481432 creator A5084283904 @default.
- W4385481432 date "2023-08-02" @default.
- W4385481432 modified "2023-09-26" @default.
- W4385481432 title "Charging of Vitreous Samples in Cryogenic Electron Microscopy Mitigated by Graphene" @default.
- W4385481432 cites W1972741425 @default.
- W4385481432 cites W1976444748 @default.
- W4385481432 cites W1981393804 @default.
- W4385481432 cites W1982864174 @default.
- W4385481432 cites W1984012857 @default.
- W4385481432 cites W1986126636 @default.
- W4385481432 cites W1988952929 @default.
- W4385481432 cites W2000614227 @default.
- W4385481432 cites W2025142332 @default.
- W4385481432 cites W2026736358 @default.
- W4385481432 cites W2026877273 @default.
- W4385481432 cites W2044798455 @default.
- W4385481432 cites W2046322930 @default.
- W4385481432 cites W2046653186 @default.
- W4385481432 cites W2046847341 @default.
- W4385481432 cites W2049321497 @default.
- W4385481432 cites W2051925784 @default.
- W4385481432 cites W2054920136 @default.
- W4385481432 cites W2066962391 @default.
- W4385481432 cites W2075824676 @default.
- W4385481432 cites W2078792724 @default.
- W4385481432 cites W2085145522 @default.
- W4385481432 cites W2104234755 @default.
- W4385481432 cites W2106222617 @default.
- W4385481432 cites W2149705812 @default.
- W4385481432 cites W2409445934 @default.
- W4385481432 cites W2498179566 @default.
- W4385481432 cites W2513086024 @default.
- W4385481432 cites W2593511418 @default.
- W4385481432 cites W2760824416 @default.
- W4385481432 cites W2765279177 @default.
- W4385481432 cites W2790176517 @default.
- W4385481432 cites W2791230784 @default.
- W4385481432 cites W2899714098 @default.
- W4385481432 cites W2901029438 @default.
- W4385481432 cites W2912325502 @default.
- W4385481432 cites W2945827648 @default.
- W4385481432 cites W2950967305 @default.
- W4385481432 cites W2966366206 @default.
- W4385481432 cites W2998470954 @default.
- W4385481432 cites W3003633907 @default.
- W4385481432 cites W3013491829 @default.
- W4385481432 cites W3032336952 @default.
- W4385481432 cites W3047071464 @default.
- W4385481432 cites W3092349029 @default.
- W4385481432 cites W3093646295 @default.
- W4385481432 cites W3094051077 @default.
- W4385481432 cites W3124036234 @default.
- W4385481432 cites W3134371113 @default.
- W4385481432 cites W3168815463 @default.
- W4385481432 cites W3190214811 @default.
- W4385481432 cites W3194321311 @default.
- W4385481432 cites W3197579449 @default.
- W4385481432 cites W3201891775 @default.
- W4385481432 cites W3214527518 @default.
- W4385481432 cites W4285393102 @default.
- W4385481432 cites W4294637036 @default.
- W4385481432 cites W4300688867 @default.
- W4385481432 cites W4311121239 @default.
- W4385481432 cites W4311669469 @default.
- W4385481432 cites W4313528735 @default.
- W4385481432 cites W4319458736 @default.
- W4385481432 cites W4320023978 @default.
- W4385481432 doi "https://doi.org/10.1021/acsnano.3c03722" @default.
- W4385481432 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37531407" @default.
- W4385481432 hasPublicationYear "2023" @default.
- W4385481432 type Work @default.
- W4385481432 citedByCount "0" @default.
- W4385481432 crossrefType "journal-article" @default.
- W4385481432 hasAuthorship W4385481432A5011638743 @default.
- W4385481432 hasAuthorship W4385481432A5013323925 @default.
- W4385481432 hasAuthorship W4385481432A5013888065 @default.
- W4385481432 hasAuthorship W4385481432A5019318669 @default.
- W4385481432 hasAuthorship W4385481432A5029504399 @default.
- W4385481432 hasAuthorship W4385481432A5037247357 @default.
- W4385481432 hasAuthorship W4385481432A5039549307 @default.
- W4385481432 hasAuthorship W4385481432A5065638633 @default.
- W4385481432 hasAuthorship W4385481432A5077999397 @default.
- W4385481432 hasAuthorship W4385481432A5083082662 @default.
- W4385481432 hasAuthorship W4385481432A5084283904 @default.
- W4385481432 hasBestOaLocation W43854814321 @default.
- W4385481432 hasConcept C120665830 @default.