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- W4385491626 abstract "Synthetic autotrophy is a promising avenue to sustainable bioproduction from CO2. Here, we use iterative laboratory evolution to generate genetically diverse autotrophic strains. We identify that just three mutations are sufficient for E. coli to grow autotrophically, when introduced alongside non-native energy (formate dehydrogenase) and carbon-fixing (Rubisco, phosphoribulokinase, carbonic anhydrase) modules. The mutated genes are involved in glycolysis (pgi), central-carbon regulation (crp), and RNA transcription (rpoB). The pgi mutation reduces the enzyme activity, thereby stabilising the carbon-fixing cycle by capping a major branching flux. The other two mutations increase the ratio of NADH/NAD+ - the cycle’s electron-donor. This study demonstrates the malleability of metabolism and evolution’s capacity to switch trophic modes on laboratory time-scales and could facilitate transforming other heterotrophic organisms into autotrophs." @default.
- W4385491626 created "2023-08-03" @default.
- W4385491626 creator A5079671055 @default.
- W4385491626 date "2023-08-02" @default.
- W4385491626 modified "2023-09-28" @default.
- W4385491626 title "eLife assessment: Autotrophic growth of E. coli is achieved by a small number of genetic changes" @default.
- W4385491626 doi "https://doi.org/10.7554/elife.88793.1.sa0" @default.
- W4385491626 hasPublicationYear "2023" @default.
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