Matches in SemOpenAlex for { <https://semopenalex.org/work/W4385503783> ?p ?o ?g. }
Showing items 1 to 79 of
79
with 100 items per page.
- W4385503783 abstract "Abstract Loop-mediated isothermal amplification (LAMP) does not require thermal cycler machines to amplify DNA and has the potential to revolutionize molecular biology by bypassing the need for sophisticated equipment. The method also has a low running cost and short turnaround times with higher sensitivity when compared with many other amplification methods. The objective behind this study was to introduce a new platform for LAMP assay with improved sensitivity. In order to do such, Leishmania spp parasites were used as the study model for the investigation purposes. A new primer set (four primers plus two extra ones) were designed and optimized for the novel LAMP assay which we further assigned the name “NESTED-LAMP”. The primers target included ITS1 gene locus of cutaneous leishmaniasis (CL) to enhance the assay sensitivity. The minimum of detection of this new assay was further compared with the traditional LAMP assay, polymerase chain reaction (PCR), and real-time PCR (RT-PCR) methods. NESTED-LAMP detected 1 parasite/µL while the conventional counterpart technique was able to detect 10 5 parasites/µL. The sensitivity of PCR and RT-PCR declined when the concentration of parasite/µL was below 10 5 and 10 2 , respectively. Furthermore, this study demonstrated 100% specificity and sensitivity regarding the NESTED-LAMP method. This method could be employed as a suitable alternative for diagnostic purposes as the results demonstrated a 10 5 -fold improvement in sensitivity for the NESTED-LAMP approach compared with the conventional counterpart technique. Also, the cost-efficiency with higher sensitivity and specificity ensures the accuracy of the results." @default.
- W4385503783 created "2023-08-03" @default.
- W4385503783 creator A5007810168 @default.
- W4385503783 creator A5012791743 @default.
- W4385503783 creator A5024465731 @default.
- W4385503783 creator A5035037766 @default.
- W4385503783 creator A5039329166 @default.
- W4385503783 creator A5051299920 @default.
- W4385503783 creator A5056229727 @default.
- W4385503783 creator A5092585623 @default.
- W4385503783 date "2023-08-02" @default.
- W4385503783 modified "2023-09-27" @default.
- W4385503783 title "The development of NESTED-LAMP method as a novel approach for diagnostic purposes while using Leishmania parasite as the study model" @default.
- W4385503783 doi "https://doi.org/10.21203/rs.3.rs-2965069/v1" @default.
- W4385503783 hasPublicationYear "2023" @default.
- W4385503783 type Work @default.
- W4385503783 citedByCount "0" @default.
- W4385503783 crossrefType "posted-content" @default.
- W4385503783 hasAuthorship W4385503783A5007810168 @default.
- W4385503783 hasAuthorship W4385503783A5012791743 @default.
- W4385503783 hasAuthorship W4385503783A5024465731 @default.
- W4385503783 hasAuthorship W4385503783A5035037766 @default.
- W4385503783 hasAuthorship W4385503783A5039329166 @default.
- W4385503783 hasAuthorship W4385503783A5051299920 @default.
- W4385503783 hasAuthorship W4385503783A5056229727 @default.
- W4385503783 hasAuthorship W4385503783A5092585623 @default.
- W4385503783 hasBestOaLocation W43855037831 @default.
- W4385503783 hasConcept C104317684 @default.
- W4385503783 hasConcept C127413603 @default.
- W4385503783 hasConcept C136764020 @default.
- W4385503783 hasConcept C153911025 @default.
- W4385503783 hasConcept C157431215 @default.
- W4385503783 hasConcept C178790620 @default.
- W4385503783 hasConcept C185592680 @default.
- W4385503783 hasConcept C21200559 @default.
- W4385503783 hasConcept C24326235 @default.
- W4385503783 hasConcept C2777563447 @default.
- W4385503783 hasConcept C41008148 @default.
- W4385503783 hasConcept C49105822 @default.
- W4385503783 hasConcept C54355233 @default.
- W4385503783 hasConcept C552990157 @default.
- W4385503783 hasConcept C60635243 @default.
- W4385503783 hasConcept C70721500 @default.
- W4385503783 hasConcept C71928629 @default.
- W4385503783 hasConcept C86803240 @default.
- W4385503783 hasConceptScore W4385503783C104317684 @default.
- W4385503783 hasConceptScore W4385503783C127413603 @default.
- W4385503783 hasConceptScore W4385503783C136764020 @default.
- W4385503783 hasConceptScore W4385503783C153911025 @default.
- W4385503783 hasConceptScore W4385503783C157431215 @default.
- W4385503783 hasConceptScore W4385503783C178790620 @default.
- W4385503783 hasConceptScore W4385503783C185592680 @default.
- W4385503783 hasConceptScore W4385503783C21200559 @default.
- W4385503783 hasConceptScore W4385503783C24326235 @default.
- W4385503783 hasConceptScore W4385503783C2777563447 @default.
- W4385503783 hasConceptScore W4385503783C41008148 @default.
- W4385503783 hasConceptScore W4385503783C49105822 @default.
- W4385503783 hasConceptScore W4385503783C54355233 @default.
- W4385503783 hasConceptScore W4385503783C552990157 @default.
- W4385503783 hasConceptScore W4385503783C60635243 @default.
- W4385503783 hasConceptScore W4385503783C70721500 @default.
- W4385503783 hasConceptScore W4385503783C71928629 @default.
- W4385503783 hasConceptScore W4385503783C86803240 @default.
- W4385503783 hasLocation W43855037831 @default.
- W4385503783 hasOpenAccess W4385503783 @default.
- W4385503783 hasPrimaryLocation W43855037831 @default.
- W4385503783 hasRelatedWork W1967467411 @default.
- W4385503783 hasRelatedWork W1992501950 @default.
- W4385503783 hasRelatedWork W2019810109 @default.
- W4385503783 hasRelatedWork W2101116249 @default.
- W4385503783 hasRelatedWork W2116837959 @default.
- W4385503783 hasRelatedWork W2167033417 @default.
- W4385503783 hasRelatedWork W2329018908 @default.
- W4385503783 hasRelatedWork W2461718593 @default.
- W4385503783 hasRelatedWork W2462475414 @default.
- W4385503783 hasRelatedWork W3096257051 @default.
- W4385503783 isParatext "false" @default.
- W4385503783 isRetracted "false" @default.
- W4385503783 workType "article" @default.