Matches in SemOpenAlex for { <https://semopenalex.org/work/W4385508429> ?p ?o ?g. }
Showing items 1 to 60 of
60
with 100 items per page.
- W4385508429 abstract "The microvasculature facilitates gas exchange, provides nutrients to cells, and regulates blood flow in response to stimuli. Vascular abnormalities are an indicator of pathology for various conditions, such as compromised vessel integrity in small vessel disease and angiogenesis in tumors. Traditional immunohistochemistry enables visualization of tissue cross-sections containing exogenously labeled vasculature. Although this approach can be utilized to quantify vascular changes within small fields-of-view, it is not a practical way to study the vasculature on the scale of whole organs. Three-dimensional (3D) imaging presents a more appropriate method to visualize the vascular architecture in tissue. Here we describe the complete protocol that we use to characterize the vasculature of different organs in mice encompassing the methods to fluorescently label vessels, optically clear tissue, collect 3D vascular images, and quantify these vascular images with a semi-automated approach. Applying this procedure of image analysis presents a method to reliably quantify and characterize vascular networks in a timely fashion. This procedure is also applicable to other methods of tissue clearing and vascular labels that generate 3D images of microvasculature." @default.
- W4385508429 created "2023-08-03" @default.
- W4385508429 creator A5077581062 @default.
- W4385508429 date "2022-08-17" @default.
- W4385508429 modified "2023-09-23" @default.
- W4385508429 title "Semi-automated protocol to quantify and characterize fluorescent three-dimensional vascular images v1" @default.
- W4385508429 cites W1969963182 @default.
- W4385508429 cites W2068391384 @default.
- W4385508429 cites W2095141942 @default.
- W4385508429 cites W3153451364 @default.
- W4385508429 cites W3165519430 @default.
- W4385508429 doi "https://doi.org/10.17504/protocols.io.j8nlkk7m1l5r/v1" @default.
- W4385508429 hasPublicationYear "2022" @default.
- W4385508429 type Work @default.
- W4385508429 citedByCount "0" @default.
- W4385508429 crossrefType "posted-content" @default.
- W4385508429 hasAuthorship W4385508429A5077581062 @default.
- W4385508429 hasBestOaLocation W43855084291 @default.
- W4385508429 hasConcept C105702510 @default.
- W4385508429 hasConcept C134018914 @default.
- W4385508429 hasConcept C136229726 @default.
- W4385508429 hasConcept C142724271 @default.
- W4385508429 hasConcept C154945302 @default.
- W4385508429 hasConcept C166397571 @default.
- W4385508429 hasConcept C2777663904 @default.
- W4385508429 hasConcept C3019347889 @default.
- W4385508429 hasConcept C36464697 @default.
- W4385508429 hasConcept C41008148 @default.
- W4385508429 hasConcept C59822182 @default.
- W4385508429 hasConcept C71924100 @default.
- W4385508429 hasConcept C86803240 @default.
- W4385508429 hasConceptScore W4385508429C105702510 @default.
- W4385508429 hasConceptScore W4385508429C134018914 @default.
- W4385508429 hasConceptScore W4385508429C136229726 @default.
- W4385508429 hasConceptScore W4385508429C142724271 @default.
- W4385508429 hasConceptScore W4385508429C154945302 @default.
- W4385508429 hasConceptScore W4385508429C166397571 @default.
- W4385508429 hasConceptScore W4385508429C2777663904 @default.
- W4385508429 hasConceptScore W4385508429C3019347889 @default.
- W4385508429 hasConceptScore W4385508429C36464697 @default.
- W4385508429 hasConceptScore W4385508429C41008148 @default.
- W4385508429 hasConceptScore W4385508429C59822182 @default.
- W4385508429 hasConceptScore W4385508429C71924100 @default.
- W4385508429 hasConceptScore W4385508429C86803240 @default.
- W4385508429 hasLocation W43855084291 @default.
- W4385508429 hasOpenAccess W4385508429 @default.
- W4385508429 hasPrimaryLocation W43855084291 @default.
- W4385508429 hasRelatedWork W1981447689 @default.
- W4385508429 hasRelatedWork W2007542858 @default.
- W4385508429 hasRelatedWork W2086228870 @default.
- W4385508429 hasRelatedWork W2141955061 @default.
- W4385508429 hasRelatedWork W2363669182 @default.
- W4385508429 hasRelatedWork W2525150146 @default.
- W4385508429 hasRelatedWork W2717381632 @default.
- W4385508429 hasRelatedWork W2895110509 @default.
- W4385508429 hasRelatedWork W2986134130 @default.
- W4385508429 hasRelatedWork W1549056443 @default.
- W4385508429 isParatext "false" @default.
- W4385508429 isRetracted "false" @default.
- W4385508429 workType "article" @default.