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- W4385514586 abstract "ABSTRACTA 34-year-old male presented with lung shadow and was asymptomatic during medical examination. The patient had a prior history of thyroid tumors. Imaging manifestation showed a nodule in the medial segment of the right middle lobe, with partial obstruction of the distal bronchus within the lesion. Ground-glass and inflammatory nodules were observed in the anterior segment of the right upper lobe, as well as chronic inflammatory changes in the lower lobe of the right lung. Lung histopathological examination suggested invasive adenocarcinoma. A morphological examination of the bronchoalveolar lavage fluid revealed the presence of Tropheryma whipplei (TW) and Nocardia. Although TW infection has been reported in cancer patients, co-infection with Nocardia is a unique occurrence in this case. Opportunistic pathogens are common in immunocompromised patients but in this case, the patient was a young adult with normal immunity and an early-stage tumor with TW and Nocardia co-infection. We demonstrated the presence of rare microorganisms through imaging findings, combined with different staining methods of bronchoalveolar lavage fluid and lung tissue sections and evaluation of morphological characteristics. The aim of the present study was to provide early diagnosis and treatment of patients by improving microbial morphological detection.KEYWORDS: Tropheryma whippleiNocardialung tumorcell morphologybronchoalveolar lavage fluid examinationopportunistic pathogen AcknowledgmentsI would like to thank the authors for their efforts in preparing this manuscript. I also thank professor Mao Wu of Zhejiang Provincial People’s Hospital for training me in morphological diagnosis over the years, Professor Weizhong Jin of Respiratory Department of Hangzhou First People’s Hospital for the guidance, and Dr. Qiong Chen and Professor Lihui Xu of Microbiology Department of Hangzhou First People’s Hospital for the bacterial culture and identification. Professor Junying Li from the Electron Microscopy Laboratory of Agricultural Health and Environmental Testing Center of Zhejiang University assisted in guiding the morphological analysis of transmission electron microscopy; Professor Haibin Wang from the Department of Radiology of Hangzhou First People’s Hospital guided the imaging; and Professor Qiaoyun Li and Professor Jian Wu from the Department of Pathology of Hangzhou First People’s Hospital guided the pathological technique.Disclosure statementNo potential conflict of interest was reported by the author(s).Data availability statementThe dataset presented in this study can be found in the article or Supplementary Materials.Ethics statementThe studies involving human participants were reviewed and approved by the Ethics Committee of Hangzhou First People’s Hospital, Zhejiang University School of Medicine. The patients/participants provided written informed consent to participate in this study. Written informed consent was obtained from individuals for the publication of any potentially identifiable images or data included in this article.Author contributionsYL Z: Data collection and data sorting. NY: Paper writing and submission. SL R: Morphological examination and case analysis.SH W: Sequencing, PCR and other experimental detection.DJ Y: Language and technical guidance, financial support. JJ Paper guidance and revision. All authors contributed to the article and approved the submitted version.Additional informationFundingThanks to the support of The 2022 Industry-University Cooperation Collaborative Education Project of the Ministry of Education(funding number:220906053235359), The Hangzhou Biomedical and Health Industry Development Support Science and Technology Project (funding number:2021WJCY090, 2021WJCY272, and Yuli Zhou. Funds were used for experimental design, image examination, next generation sequencing, fluorescence quantitative PCR, conventional bronchoalveolar lavage fluid morphology examination (including Regis staining, weak acid staining, and glycogen staining), lung histopathology examination (including immunohistochemistry, HE staining, glycogen staining, hexamine silver staining), transmission electron microscopy, and other consumables and equipment costs, English polishing, review and layout costs, and transportation costs, among others." @default.
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- W4385514586 date "2023-08-02" @default.
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- W4385514586 title "Lung cancer patient with <i>Tropheryma whipplei</i> and <i>Nocardia</i> co-infection" @default.
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- W4385514586 doi "https://doi.org/10.1080/01913123.2023.2241888" @default.
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