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- W4385514882 abstract "Fluorine labeling of ribonucleic acids (RNA) in conjunction with 19 F NMR spectroscopy has emerged as a powerful strategy for spectroscopic analysis of RNA structure and dynamics, and RNA-ligand interactions. This study presents the first syntheses of 2'-OCF3 guanosine and uridine phosphoramidites, their incorporation into oligoribonucleotides by solid-phase synthesis and a comprehensive study of their properties. NMR spectroscopic analysis showed that the 2'-OCF3 modification is associated with preferential C2'-endo conformation of the U and G ribose in single-stranded RNA. When paired to the complementary strand, slight destabilization of the duplex caused by the modification was revealed by UV melting curve analysis. Moreover, the power of the 2'-OCF3 label for NMR spectroscopy is demonstrated by dissecting RNA pseudoknot folding and its binding to a small molecule. Furthermore, the 2'-OCF3 modification has potential for applications in therapeutic oligonucleotides. To this end, three 2'-OCF3 modified siRNAs were tested in silencing of the BASP1 gene which indicated enhanced performance for one of them. Importantly, together with earlier work, the present study completes the set of 2'-OCF3 nucleoside phosphoramidites to all four standard nucleobases (A, U, C, G) and hence enables applications that utilize the favorable properties of the 2'-OCF3 group without any restrictions in placing the modification into the RNA target sequence." @default.
- W4385514882 created "2023-08-04" @default.
- W4385514882 creator A5008760265 @default.
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- W4385514882 date "2023-09-20" @default.
- W4385514882 modified "2023-10-18" @default.
- W4385514882 title "Advances in RNA Labeling with Trifluoromethyl Groups" @default.
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- W4385514882 doi "https://doi.org/10.1002/chem.202302220" @default.
- W4385514882 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37534701" @default.
- W4385514882 hasPublicationYear "2023" @default.
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