Matches in SemOpenAlex for { <https://semopenalex.org/work/W4385544656> ?p ?o ?g. }
- W4385544656 endingPage "12399" @default.
- W4385544656 startingPage "12399" @default.
- W4385544656 abstract "The evidence sustaining the regenerative properties of mesenchymal stem cells' (MSCs) secretome has prompted a paradigm change, where MSCs have shifted from being considered direct contributors to tissue regeneration toward being seen as cell factories for producing biotech medicines. We have previously designed a method to prime MSCs towards osteogenic differentiation by silencing the Wnt/β-Catenin inhibitor Sfpr1. This approach produces a significant increase in bone formation in osteoporotic mice. In this current work, we set to investigate the contribution of the secretome from the MSCs where Sfrp1 has been silenced, to the positive effect seen on bone regeneration in vivo. The conditioned media (CM) of the murine MSCs line C3H10T1/2, where Sfrp1 has been transiently silenced (CM-Sfrp1), was found to induce, in vitro, an increase in the osteogenic differentiation of this same cell line, as well as a decrease of the expression of the Wnt inhibitor Dkk1 in murine osteocytes ex vivo. A reduction in the RANKL/OPG ratio was also detected ex vivo, suggesting a negative effect of CM-Sfrp1 on osteoclastogenesis. Moreover, this CM significantly increases the mineralization of human primary MSCs isolated from osteoportotic patients in vitro. Proteomic analysis identified enrichment of proteins involved in osteogenesis within the soluble and vesicular fractions of this secretome. Altogether, we demonstrate the pro-osteogenic potential of the secretome of MSCs primmed in this fashion, suggesting that this is a valid approach to enhance the osteo-regenerative properties of MSCs' secretome." @default.
- W4385544656 created "2023-08-04" @default.
- W4385544656 creator A5006097055 @default.
- W4385544656 creator A5010214359 @default.
- W4385544656 creator A5033788506 @default.
- W4385544656 creator A5050631176 @default.
- W4385544656 creator A5068112563 @default.
- W4385544656 creator A5081414895 @default.
- W4385544656 creator A5082077932 @default.
- W4385544656 creator A5083902061 @default.
- W4385544656 creator A5085021759 @default.
- W4385544656 creator A5092017377 @default.
- W4385544656 creator A5092591332 @default.
- W4385544656 date "2023-08-03" @default.
- W4385544656 modified "2023-10-01" @default.
- W4385544656 title "Engineering a Pro-Osteogenic Secretome through the Transient Silencing of the Gene Encoding Secreted Frizzled Related Protein 1" @default.
- W4385544656 cites W1607395428 @default.
- W4385544656 cites W1968628114 @default.
- W4385544656 cites W1991683376 @default.
- W4385544656 cites W2021019297 @default.
- W4385544656 cites W2024941123 @default.
- W4385544656 cites W2055559862 @default.
- W4385544656 cites W2056138252 @default.
- W4385544656 cites W2064729399 @default.
- W4385544656 cites W2088707712 @default.
- W4385544656 cites W2093252878 @default.
- W4385544656 cites W2130835350 @default.
- W4385544656 cites W2140710747 @default.
- W4385544656 cites W2151407094 @default.
- W4385544656 cites W2231350081 @default.
- W4385544656 cites W2239874339 @default.
- W4385544656 cites W2463195069 @default.
- W4385544656 cites W2567374865 @default.
- W4385544656 cites W2595012589 @default.
- W4385544656 cites W2746710261 @default.
- W4385544656 cites W2791332544 @default.
- W4385544656 cites W2806705495 @default.
- W4385544656 cites W28112311 @default.
- W4385544656 cites W2885316716 @default.
- W4385544656 cites W2981462562 @default.
- W4385544656 cites W2986649250 @default.
- W4385544656 cites W2991106430 @default.
- W4385544656 cites W2995050577 @default.
- W4385544656 cites W3006136716 @default.
- W4385544656 cites W3006279369 @default.
- W4385544656 cites W3116805375 @default.
- W4385544656 cites W3119998677 @default.
- W4385544656 cites W3164270472 @default.
- W4385544656 cites W3195337187 @default.
- W4385544656 cites W4213390865 @default.
- W4385544656 cites W4307949690 @default.
- W4385544656 doi "https://doi.org/10.3390/ijms241512399" @default.
- W4385544656 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37569774" @default.
- W4385544656 hasPublicationYear "2023" @default.
- W4385544656 type Work @default.
- W4385544656 citedByCount "0" @default.
- W4385544656 crossrefType "journal-article" @default.
- W4385544656 hasAuthorship W4385544656A5006097055 @default.
- W4385544656 hasAuthorship W4385544656A5010214359 @default.
- W4385544656 hasAuthorship W4385544656A5033788506 @default.
- W4385544656 hasAuthorship W4385544656A5050631176 @default.
- W4385544656 hasAuthorship W4385544656A5068112563 @default.
- W4385544656 hasAuthorship W4385544656A5081414895 @default.
- W4385544656 hasAuthorship W4385544656A5082077932 @default.
- W4385544656 hasAuthorship W4385544656A5083902061 @default.
- W4385544656 hasAuthorship W4385544656A5085021759 @default.
- W4385544656 hasAuthorship W4385544656A5092017377 @default.
- W4385544656 hasAuthorship W4385544656A5092591332 @default.
- W4385544656 hasBestOaLocation W43855446561 @default.
- W4385544656 hasConcept C104317684 @default.
- W4385544656 hasConcept C119056186 @default.
- W4385544656 hasConcept C137620995 @default.
- W4385544656 hasConcept C150903083 @default.
- W4385544656 hasConcept C185592680 @default.
- W4385544656 hasConcept C198826908 @default.
- W4385544656 hasConcept C207001950 @default.
- W4385544656 hasConcept C26291073 @default.
- W4385544656 hasConcept C2779428903 @default.
- W4385544656 hasConcept C55493867 @default.
- W4385544656 hasConcept C62478195 @default.
- W4385544656 hasConcept C86803240 @default.
- W4385544656 hasConcept C88045685 @default.
- W4385544656 hasConcept C95444343 @default.
- W4385544656 hasConceptScore W4385544656C104317684 @default.
- W4385544656 hasConceptScore W4385544656C119056186 @default.
- W4385544656 hasConceptScore W4385544656C137620995 @default.
- W4385544656 hasConceptScore W4385544656C150903083 @default.
- W4385544656 hasConceptScore W4385544656C185592680 @default.
- W4385544656 hasConceptScore W4385544656C198826908 @default.
- W4385544656 hasConceptScore W4385544656C207001950 @default.
- W4385544656 hasConceptScore W4385544656C26291073 @default.
- W4385544656 hasConceptScore W4385544656C2779428903 @default.
- W4385544656 hasConceptScore W4385544656C55493867 @default.
- W4385544656 hasConceptScore W4385544656C62478195 @default.
- W4385544656 hasConceptScore W4385544656C86803240 @default.
- W4385544656 hasConceptScore W4385544656C88045685 @default.
- W4385544656 hasConceptScore W4385544656C95444343 @default.
- W4385544656 hasFunder F4320334923 @default.