FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4385546049> ?p ?o ?g. }

• W4385546049 abstract "ATP, an important cofactor, is involved in many biocatalytic reactions that require energy. Polyphosphate kinases (PPK) can provide energy for ATP-consuming reactions due to their cheap and readily available substrate polyphosphate. We determined the catalytic properties of PPK from different sources and found that PPK from Cytophaga hutchinsonii (ChPPK) had the best catalytic activity for the substrates ADP and polyP6. An extracellular-intracellular dual system was constructed to high-throughput screen for better catalytic activity of ChPPK mutants. Finally, the specific activity of ChPPKD82N-K103E mutant was increased by 4.3 times. Therefore, we focused on the production of L-theanine catalyzed by GMAS as a model of ATP regeneration. Supplying 150 mM ATP, GMAS enzyme could produce 16.8 ± 1.3 g/L L-theanine from 100 mM glutamate. When 5 mM ATP and 5 U/mL ChPPKD82N-K103E were added, the yield of L-theanine was 16.6 ± 0.79 g/L with the conversion rate of 95.6 ± 4.5% at 4 h. Subsequently, this system was scaled up to 200 mM and 400 mM glutamate, resulting in the yields of L-theanine for 32.3 ± 1.6 g/L and 62.7 ± 1.1 g/L, with the conversion rate of 92.8 ± 4.6% and 90.1 ± 1.6%, respectively. In addition, we also constructed an efficient ATP regeneration system from glutamate to glutamine, and 13.8 ± 0.2 g/L glutamine was obtained with the conversion rate of 94.4 ± 1.4% in 4 h after adding 6 U/ mL GS enzyme and 5 U/ mL ChPPKD82N-K103E, which further laid the foundation from glutamine to L-theanine catalyzed by GGT enzyme. This proved that giving the reaction an efficient ATP supply driven by the mutant enzyme enhanced the conversion rate of substrate to product and maximized the substrate value. This is a positively combination of high yield, high conversion rate and high economic value of enzyme catalysis. The mutant enzyme will further power the ATP-consuming biocatalytic reaction platform sustainably." @default.
• W4385546049 created "2023-08-04" @default.
• W4385546049 creator A5021412121 @default.
• W4385546049 creator A5037146416 @default.
• W4385546049 creator A5045322000 @default.
• W4385546049 creator A5053885380 @default.
• W4385546049 creator A5072315367 @default.
• W4385546049 creator A5074322926 @default.
• W4385546049 creator A5074999356 @default.
• W4385546049 creator A5077886604 @default.
• W4385546049 date "2023-08-03" @default.
• W4385546049 modified "2023-10-07" @default.
• W4385546049 title "A high-throughput dual system to screen polyphosphate kinase mutants for efficient ATP regeneration in L-theanine biocatalysis" @default.
• W4385546049 cites W1965322645 @default.
• W4385546049 cites W2005920747 @default.
• W4385546049 cites W2009378048 @default.
• W4385546049 cites W2009382819 @default.
• W4385546049 cites W2039589393 @default.
• W4385546049 cites W2087888443 @default.
• W4385546049 cites W2117288172 @default.
• W4385546049 cites W2123233366 @default.
• W4385546049 cites W2128635872 @default.
• W4385546049 cites W2136479814 @default.
• W4385546049 cites W2149614036 @default.
• W4385546049 cites W2155596249 @default.
• W4385546049 cites W2275597726 @default.
• W4385546049 cites W2300537338 @default.
• W4385546049 cites W2317855082 @default.
• W4385546049 cites W2418686223 @default.
• W4385546049 cites W2538176089 @default.
• W4385546049 cites W2549548392 @default.
• W4385546049 cites W2897362377 @default.
• W4385546049 cites W2904141526 @default.
• W4385546049 cites W2937930432 @default.
• W4385546049 cites W2972592687 @default.
• W4385546049 cites W2990839708 @default.
• W4385546049 cites W2998073984 @default.
• W4385546049 cites W3009423754 @default.
• W4385546049 cites W3011997371 @default.
• W4385546049 cites W3049591605 @default.
• W4385546049 cites W3090968839 @default.
• W4385546049 cites W3113879247 @default.
• W4385546049 cites W3121090268 @default.
• W4385546049 cites W3129242442 @default.
• W4385546049 cites W3169096969 @default.
• W4385546049 cites W3195736944 @default.
• W4385546049 cites W3207068342 @default.
• W4385546049 cites W4200600110 @default.
• W4385546049 cites W4221122864 @default.
• W4385546049 cites W4288070074 @default.
• W4385546049 cites W4293247451 @default.
• W4385546049 doi "https://doi.org/10.1186/s13068-023-02361-9" @default.
• W4385546049 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37537682" @default.
• W4385546049 hasPublicationYear "2023" @default.
• W4385546049 type Work @default.
• W4385546049 citedByCount "1" @default.
• W4385546049 countsByYear W43855460492023 @default.
• W4385546049 crossrefType "journal-article" @default.
• W4385546049 hasAuthorship W4385546049A5021412121 @default.
• W4385546049 hasAuthorship W4385546049A5037146416 @default.
• W4385546049 hasAuthorship W4385546049A5045322000 @default.
• W4385546049 hasAuthorship W4385546049A5053885380 @default.
• W4385546049 hasAuthorship W4385546049A5072315367 @default.
• W4385546049 hasAuthorship W4385546049A5074322926 @default.
• W4385546049 hasAuthorship W4385546049A5074999356 @default.
• W4385546049 hasAuthorship W4385546049A5077886604 @default.
• W4385546049 hasBestOaLocation W43855460491 @default.
• W4385546049 hasConcept C104317684 @default.
• W4385546049 hasConcept C134121241 @default.
• W4385546049 hasConcept C143065580 @default.
• W4385546049 hasConcept C181199279 @default.
• W4385546049 hasConcept C185592680 @default.
• W4385546049 hasConcept C191897082 @default.
• W4385546049 hasConcept C192562407 @default.
• W4385546049 hasConcept C197957613 @default.
• W4385546049 hasConcept C2777132085 @default.
• W4385546049 hasConcept C2778038992 @default.
• W4385546049 hasConcept C2779282468 @default.
• W4385546049 hasConcept C2779289688 @default.
• W4385546049 hasConcept C2779349466 @default.
• W4385546049 hasConcept C2992814287 @default.
• W4385546049 hasConcept C31903555 @default.
• W4385546049 hasConcept C515207424 @default.
• W4385546049 hasConcept C55493867 @default.
• W4385546049 hasConceptScore W4385546049C104317684 @default.
• W4385546049 hasConceptScore W4385546049C134121241 @default.
• W4385546049 hasConceptScore W4385546049C143065580 @default.
• W4385546049 hasConceptScore W4385546049C181199279 @default.
• W4385546049 hasConceptScore W4385546049C185592680 @default.
• W4385546049 hasConceptScore W4385546049C191897082 @default.
• W4385546049 hasConceptScore W4385546049C192562407 @default.
• W4385546049 hasConceptScore W4385546049C197957613 @default.
• W4385546049 hasConceptScore W4385546049C2777132085 @default.
• W4385546049 hasConceptScore W4385546049C2778038992 @default.
• W4385546049 hasConceptScore W4385546049C2779282468 @default.
• W4385546049 hasConceptScore W4385546049C2779289688 @default.
• W4385546049 hasConceptScore W4385546049C2779349466 @default.
• W4385546049 hasConceptScore W4385546049C2992814287 @default.
• W4385546049 hasConceptScore W4385546049C31903555 @default.
• W4385546049 hasConceptScore W4385546049C515207424 @default.

• next