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• W4385555870 abstract "Abstract Here we present a method to reduce the photobleaching of fluorescent proteins and the associated phototoxicity. It exploits a photophysical process known as reverse intersystem crossing, which we induce by near-infrared co-illumination during fluorophore excitation. This dual illumination method reduces photobleaching effects 1.5–9.2-fold, can be easily implemented on commercial microscopes and is effective in eukaryotic and prokaryotic cells with a wide range of fluorescent proteins." @default.
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• W4385555870 date "2023-08-03" @default.
• W4385555870 modified "2023-10-14" @default.
• W4385555870 title "Near-infrared co-illumination of fluorescent proteins reduces photobleaching and phototoxicity" @default.
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• W4385555870 doi "https://doi.org/10.1038/s41587-023-01893-7" @default.
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