FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4385575319> ?p ?o ?g. }

• W4385575319 endingPage "1239" @default.
• W4385575319 startingPage "1230" @default.
• W4385575319 abstract "Malignant pleural mesothelioma (MPM), a rare and aggressive pleural tumor, has significant histological and molecular heterogeneity. Primary Cilium (PC), an organelle of emerging importance in malignancies, has been scarcely investigated in MPM. A critical molecular complex for the PC function is the BBSome and here we aimed at assessing its expression patterns in ordinary 2D and spheroid 3D cell cultures.A human benign mesothelial cell line (MeT-5A), MPM cell lines (M14K, epithelioid MPM; MSTO, biphasic MPM), and primary MPM cells (pMPM) were used. Primers specific for the human BBS1, 2, 4, 5, 7, 9, 18 transcripts were designed, and quantitative real-time PCR (qRT-PCR) was done with β-actin as the gene of reference. The relative gene expression across 2D and 3D cultures was analyzed by the expression factor (mean of 1/ΔCt values). With the 2-∆∆Ct method the gene expression fold changes were assessed from qRT-PCR data. Molecular changes using the PC-modulating drugs ammonium sulfate (AS) and lithium chloride (LC) were also determined.PC was present in all cells used in the study at approximately 15% of the observed area. BBSome transcripts were differentially expressed in different dimensions of cell culture (2D vs. 3D) in all cell lines and pMPM. Treatment with AS and LC affected the expression of the ciliary BBS2 and BBS18 genes in the benign as well as in the MPM cells.These data indicate distinct BBSome molecular profiles in human benign and MPM cells cultured in 2D and 3D dimensions and support the notion that PC genes should be investigated as potential MPM therapeutic targets." @default.
• W4385575319 created "2023-08-05" @default.
• W4385575319 creator A5006222096 @default.
• W4385575319 creator A5043872084 @default.
• W4385575319 creator A5047523218 @default.
• W4385575319 creator A5048262795 @default.
• W4385575319 creator A5050627759 @default.
• W4385575319 creator A5062468858 @default.
• W4385575319 creator A5064983758 @default.
• W4385575319 creator A5069152924 @default.
• W4385575319 creator A5072637382 @default.
• W4385575319 creator A5076825236 @default.
• W4385575319 creator A5081395852 @default.
• W4385575319 creator A5083460546 @default.
• W4385575319 creator A5084840836 @default.
• W4385575319 date "2023-08-04" @default.
• W4385575319 modified "2023-10-17" @default.
• W4385575319 title "Changes in expression of mesothelial BBS genes in 2D and 3D after lithium chloride and ammonium sulphate induction of primary cilium disturbance: a pilot study" @default.
• W4385575319 cites W1979921735 @default.
• W4385575319 cites W1997241984 @default.
• W4385575319 cites W2007541023 @default.
• W4385575319 cites W2048996150 @default.
• W4385575319 cites W2105996541 @default.
• W4385575319 cites W2121593051 @default.
• W4385575319 cites W2124555630 @default.
• W4385575319 cites W2167279371 @default.
• W4385575319 cites W2210480170 @default.
• W4385575319 cites W2239938647 @default.
• W4385575319 cites W2247983356 @default.
• W4385575319 cites W2280795258 @default.
• W4385575319 cites W2291531085 @default.
• W4385575319 cites W2559224373 @default.
• W4385575319 cites W2583723694 @default.
• W4385575319 cites W2766056185 @default.
• W4385575319 cites W2808091376 @default.
• W4385575319 cites W2909273376 @default.
• W4385575319 cites W2924341172 @default.
• W4385575319 cites W2961780602 @default.
• W4385575319 cites W2980709198 @default.
• W4385575319 cites W2996129112 @default.
• W4385575319 cites W3000146196 @default.
• W4385575319 cites W3036637668 @default.
• W4385575319 cites W3042684909 @default.
• W4385575319 cites W3094109421 @default.
• W4385575319 cites W3098635819 @default.
• W4385575319 cites W3113043350 @default.
• W4385575319 cites W3125391706 @default.
• W4385575319 cites W3165747411 @default.
• W4385575319 cites W4200227712 @default.
• W4385575319 cites W4210690777 @default.
• W4385575319 cites W4220910075 @default.
• W4385575319 cites W4225274280 @default.
• W4385575319 cites W4296526485 @default.
• W4385575319 cites W4296793422 @default.
• W4385575319 cites W4307556068 @default.
• W4385575319 cites W4323308214 @default.
• W4385575319 cites W807932216 @default.
• W4385575319 doi "https://doi.org/10.1007/s43440-023-00513-0" @default.
• W4385575319 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37542187" @default.
• W4385575319 hasPublicationYear "2023" @default.
• W4385575319 type Work @default.
• W4385575319 citedByCount "0" @default.
• W4385575319 crossrefType "journal-article" @default.
• W4385575319 hasAuthorship W4385575319A5006222096 @default.
• W4385575319 hasAuthorship W4385575319A5043872084 @default.
• W4385575319 hasAuthorship W4385575319A5047523218 @default.
• W4385575319 hasAuthorship W4385575319A5048262795 @default.
• W4385575319 hasAuthorship W4385575319A5050627759 @default.
• W4385575319 hasAuthorship W4385575319A5062468858 @default.
• W4385575319 hasAuthorship W4385575319A5064983758 @default.
• W4385575319 hasAuthorship W4385575319A5069152924 @default.
• W4385575319 hasAuthorship W4385575319A5072637382 @default.
• W4385575319 hasAuthorship W4385575319A5076825236 @default.
• W4385575319 hasAuthorship W4385575319A5081395852 @default.
• W4385575319 hasAuthorship W4385575319A5083460546 @default.
• W4385575319 hasAuthorship W4385575319A5084840836 @default.
• W4385575319 hasBestOaLocation W43855753191 @default.
• W4385575319 hasConcept C104317684 @default.
• W4385575319 hasConcept C150194340 @default.
• W4385575319 hasConcept C153911025 @default.
• W4385575319 hasConcept C185592680 @default.
• W4385575319 hasConcept C54355233 @default.
• W4385575319 hasConcept C81885089 @default.
• W4385575319 hasConcept C86803240 @default.
• W4385575319 hasConcept C95444343 @default.
• W4385575319 hasConceptScore W4385575319C104317684 @default.
• W4385575319 hasConceptScore W4385575319C150194340 @default.
• W4385575319 hasConceptScore W4385575319C153911025 @default.
• W4385575319 hasConceptScore W4385575319C185592680 @default.
• W4385575319 hasConceptScore W4385575319C54355233 @default.
• W4385575319 hasConceptScore W4385575319C81885089 @default.
• W4385575319 hasConceptScore W4385575319C86803240 @default.
• W4385575319 hasConceptScore W4385575319C95444343 @default.
• W4385575319 hasFunder F4320327859 @default.
• W4385575319 hasIssue "5" @default.
• W4385575319 hasLocation W43855753191 @default.
• W4385575319 hasLocation W43855753192 @default.
• W4385575319 hasOpenAccess W4385575319 @default.

• next