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• W4385583345 abstract "In nature, frost can form at a few degrees below zero Celsius. However, this process requires the assembly of tens of thousands of ice-like water molecules that align together to initiate freezing at these relatively high temperatures. Water ordering on this scale is mediated by the ice nucleation proteins of common environmental bacteria like Pseudomonas syringae and P. borealis . However, individually, these 100-kDa proteins are too small to organize enough water molecules for frost formation, and it is not known how giant, megadalton-sized multimers, which are crucial for ice nucleation at high sub-zero temperatures, form. The ability of multimers to self-assemble was suggested when the transfer of an ice nucleation protein gene into E. coli led to efficient ice nucleation. Here we demonstrate that a positively-charged sub-domain at the C-terminal end of the central beta-solenoid of the ice nucleation protein is crucial for multimerization. Truncation, relocation, or change of the charge of this subdomain caused a catastrophic loss of ice nucleation ability. Cryo-electron tomography of the recombinant E. coli showed that the ice nucleation protein multimers form fibres that are ∼ 5 nm across and up to 200 nm long. A model of these fibres as an overlapping series of antiparallel dimers can account for all their known properties and suggests a route to making cell-free ice nucleators for biotechnological applications." @default.
• W4385583345 created "2023-08-05" @default.
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• W4385583345 date "2023-08-03" @default.
• W4385583345 modified "2023-09-27" @default.
• W4385583345 title "Ice nucleation proteins self-assemble into large fibres to trigger freezing at near 0 ℃" @default.
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• W4385583345 doi "https://doi.org/10.1101/2023.08.03.551873" @default.
• W4385583345 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37577566" @default.
• W4385583345 hasPublicationYear "2023" @default.
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