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• W4385588019 abstract "Traditional methods of gamete handling, fertilization, and embryo culture often face limitations in efficiency, consistency, and the ability to closely mimic in vivo conditions. This review explores the opportunities presented by microfluidic and 3D culture systems in overcoming these challenges and enhancing in vitro embryo production. We discuss the basic principles of microfluidics, emphasizing their inherent advantages such as precise control of fluid flow, reduced reagent consumption, and high-throughput capabilities. Furthermore, we delve into microfluidic devices designed for gamete manipulation, in vitro fertilization, and embryo culture, highlighting innovations such as droplet-based microfluidics and on-chip monitoring. Next, we explore the integration of 3D culture systems, including the use of biomimetic scaffolds and organ-on-a-chip platforms, with a particular focus on the oviduct-on-a-chip. Finally, we discuss the potential of these advanced systems to improve embryo production outcomes and advance our understanding of early embryo development. By leveraging the unique capabilities of microfluidics and 3D culture systems, we foresee significant advancements in the efficiency, effectiveness, and clinical success of in vitro embryo production." @default.
• W4385588019 created "2023-08-05" @default.
• W4385588019 creator A5047101186 @default.
• W4385588019 creator A5061244299 @default.
• W4385588019 date "2023-01-01" @default.
• W4385588019 modified "2023-10-14" @default.
• W4385588019 title "Opportunities involving microfluidics and 3D culture systems to the in vitro embryo production" @default.
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• W4385588019 doi "https://doi.org/10.1590/1984-3143-ar2023-0058" @default.
• W4385588019 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37638255" @default.

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