Matches in SemOpenAlex for { <https://semopenalex.org/work/W4385623367> ?p ?o ?g. }
Showing items 1 to 97 of
97
with 100 items per page.
- W4385623367 endingPage "105659" @default.
- W4385623367 startingPage "105659" @default.
- W4385623367 abstract "The H295R test guideline assay evaluates the effect of test substances on synthesis of 17β-estradiol (E2) and testosterone (T). The objective of this study was to leverage commercial immunoassay technology to develop a more efficient H295R assay to measure E2 and T levels in 384-well format. The resulting Homogenous Time Resolved Fluorescence assay platform (H295R-HTRF) was evaluated against a training set of 36 chemicals derived from the OECD inter-laboratory validation study, EPA guideline 890.1200 aromatase assay, and azole fungicides active in the HT-H295R assay. Quality control performance criteria were met for all conditions except E2 synthesis inhibition where low basal hormone synthesis was observed. Five proficiency chemicals were active for both the E2 and T endpoints, consistent with guideline classifications. Of the nine OECD core reference chemicals, 9/9 were concordant with outcomes for E2 and 7/9 for T. Likewise, 9/13 and 11/13 OECD supplemental chemicals were concordant with anticipated effects for E2 and T, respectively. Of the 10 azole fungicides screened, 7/10 for E2 and 8/10 for T exhibited concordant outcomes for inhibition. Generally, all active chemicals in the training set demonstrated equivalent or greater potency in the H295R-HTRF assay, supporting the sensitivity of the platform. The adaptation of HTRF technology to the H295R model provides an efficient way to evaluate E2 and T modulators in accordance with guideline specifications." @default.
- W4385623367 created "2023-08-08" @default.
- W4385623367 creator A5009568925 @default.
- W4385623367 creator A5017646613 @default.
- W4385623367 creator A5036400318 @default.
- W4385623367 creator A5051234462 @default.
- W4385623367 creator A5066493268 @default.
- W4385623367 creator A5092604173 @default.
- W4385623367 date "2023-10-01" @default.
- W4385623367 modified "2023-10-16" @default.
- W4385623367 title "Evaluation of a high-throughput H295R homogenous time resolved fluorescence assay for androgen and estrogen steroidogenesis screening" @default.
- W4385623367 cites W1593195133 @default.
- W4385623367 cites W2004429971 @default.
- W4385623367 cites W2012133139 @default.
- W4385623367 cites W2015229715 @default.
- W4385623367 cites W2017724775 @default.
- W4385623367 cites W2031348353 @default.
- W4385623367 cites W2035266313 @default.
- W4385623367 cites W2051175597 @default.
- W4385623367 cites W2059539545 @default.
- W4385623367 cites W2073113196 @default.
- W4385623367 cites W2118887433 @default.
- W4385623367 cites W2122025333 @default.
- W4385623367 cites W2126168000 @default.
- W4385623367 cites W2133314499 @default.
- W4385623367 cites W2133556122 @default.
- W4385623367 cites W2134895776 @default.
- W4385623367 cites W2142377443 @default.
- W4385623367 cites W2311044780 @default.
- W4385623367 cites W2611143514 @default.
- W4385623367 cites W2772752440 @default.
- W4385623367 cites W2919637418 @default.
- W4385623367 cites W2982494776 @default.
- W4385623367 cites W3129067354 @default.
- W4385623367 cites W4295777992 @default.
- W4385623367 doi "https://doi.org/10.1016/j.tiv.2023.105659" @default.
- W4385623367 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37557933" @default.
- W4385623367 hasPublicationYear "2023" @default.
- W4385623367 type Work @default.
- W4385623367 citedByCount "0" @default.
- W4385623367 crossrefType "journal-article" @default.
- W4385623367 hasAuthorship W4385623367A5009568925 @default.
- W4385623367 hasAuthorship W4385623367A5017646613 @default.
- W4385623367 hasAuthorship W4385623367A5036400318 @default.
- W4385623367 hasAuthorship W4385623367A5051234462 @default.
- W4385623367 hasAuthorship W4385623367A5066493268 @default.
- W4385623367 hasAuthorship W4385623367A5092604173 @default.
- W4385623367 hasBestOaLocation W43856233671 @default.
- W4385623367 hasConcept C121608353 @default.
- W4385623367 hasConcept C126322002 @default.
- W4385623367 hasConcept C142724271 @default.
- W4385623367 hasConcept C185592680 @default.
- W4385623367 hasConcept C2776166826 @default.
- W4385623367 hasConcept C2777164284 @default.
- W4385623367 hasConcept C2779548794 @default.
- W4385623367 hasConcept C2780182762 @default.
- W4385623367 hasConcept C2780315139 @default.
- W4385623367 hasConcept C530470458 @default.
- W4385623367 hasConcept C71924100 @default.
- W4385623367 hasConcept C86803240 @default.
- W4385623367 hasConcept C89423630 @default.
- W4385623367 hasConcept C98274493 @default.
- W4385623367 hasConceptScore W4385623367C121608353 @default.
- W4385623367 hasConceptScore W4385623367C126322002 @default.
- W4385623367 hasConceptScore W4385623367C142724271 @default.
- W4385623367 hasConceptScore W4385623367C185592680 @default.
- W4385623367 hasConceptScore W4385623367C2776166826 @default.
- W4385623367 hasConceptScore W4385623367C2777164284 @default.
- W4385623367 hasConceptScore W4385623367C2779548794 @default.
- W4385623367 hasConceptScore W4385623367C2780182762 @default.
- W4385623367 hasConceptScore W4385623367C2780315139 @default.
- W4385623367 hasConceptScore W4385623367C530470458 @default.
- W4385623367 hasConceptScore W4385623367C71924100 @default.
- W4385623367 hasConceptScore W4385623367C86803240 @default.
- W4385623367 hasConceptScore W4385623367C89423630 @default.
- W4385623367 hasConceptScore W4385623367C98274493 @default.
- W4385623367 hasFunder F4320306107 @default.
- W4385623367 hasLocation W43856233671 @default.
- W4385623367 hasLocation W43856233672 @default.
- W4385623367 hasOpenAccess W4385623367 @default.
- W4385623367 hasPrimaryLocation W43856233671 @default.
- W4385623367 hasRelatedWork W1972642439 @default.
- W4385623367 hasRelatedWork W1984954134 @default.
- W4385623367 hasRelatedWork W2004151698 @default.
- W4385623367 hasRelatedWork W2057515305 @default.
- W4385623367 hasRelatedWork W2070975009 @default.
- W4385623367 hasRelatedWork W2151426978 @default.
- W4385623367 hasRelatedWork W2171857595 @default.
- W4385623367 hasRelatedWork W2926335606 @default.
- W4385623367 hasRelatedWork W4212763152 @default.
- W4385623367 hasRelatedWork W4231984378 @default.
- W4385623367 hasVolume "92" @default.
- W4385623367 isParatext "false" @default.
- W4385623367 isRetracted "false" @default.
- W4385623367 workType "article" @default.