FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4385647912> ?p ?o ?g. }

• W4385647912 abstract "Effective chromosome synapsis and crossover formation during meiosis are essential for fertility, especially in grain crops such as wheat. These processes function most efficiently in wheat at temperatures between 17-23 °C, although the genetic mechanisms for such temperature dependence are unknown. In a previously identified mutant of the hexaploid wheat reference variety 'Chinese Spring' lacking the long arm of chromosome 5D, exposure to low temperatures during meiosis resulted in asynapsis and crossover failure. In a second mutant (ttmei1), containing a 4 Mb deletion in chromosome 5DL, exposure to 13 °C led to similarly high levels of asynapsis and univalence. Moreover, exposure to 30 °C led to a significant, but less extreme effect on crossovers. Previously, we proposed that, of 41 genes deleted in this 4 Mb region, the major meiotic gene TaDMC1-D1 was the most likely candidate for preservation of synapsis and crossovers at low (and possibly high) temperatures. In the current study, using RNA-guided Cas9, we developed a new Chinese Spring CRISPR mutant, containing a 39 bp deletion in the 5D copy of DMC1, representing the first reported CRISPR-Cas9 targeted mutagenesis in Chinese Spring, and the first CRISPR mutant for DMC1 in wheat. In controlled environment experiments, wild-type Chinese Spring, CRISPR dmc1-D1 and backcrossed ttmei1 mutants were exposed to either high or low temperatures during the temperature-sensitive period from premeiotic interphase to early meiosis I. After 6-7 days at 13 °C, crossovers decreased by over 95% in the dmc1-D1 mutants, when compared with wild-type plants grown under the same conditions. After 24 hours at 30 °C, dmc1-D1 mutants exhibited a reduced number of crossovers and increased univalence, although these differences were less marked than at 13 °C. Similar results were obtained for ttmei1 mutants, although their scores were more variable, possibly reflecting higher levels of background mutation. These experiments confirm our previous hypothesis that DMC1-D1 is responsible for preservation of normal crossover formation at low and, to a certain extent, high temperatures. Given that reductions in crossovers have significant effects on grain yield, these results have important implications for wheat breeding, particularly in the face of climate change." @default.
• W4385647912 created "2023-08-09" @default.
• W4385647912 creator A5012225970 @default.
• W4385647912 creator A5034719020 @default.
• W4385647912 creator A5045940717 @default.
• W4385647912 creator A5055268602 @default.
• W4385647912 creator A5057081627 @default.
• W4385647912 creator A5059670375 @default.
• W4385647912 date "2023-08-08" @default.
• W4385647912 modified "2023-10-14" @default.
• W4385647912 title "DMC1 stabilizes crossovers at high and low temperatures during wheat meiosis" @default.
• W4385647912 cites W1925156307 @default.
• W4385647912 cites W1965740284 @default.
• W4385647912 cites W1966369722 @default.
• W4385647912 cites W1971660736 @default.
• W4385647912 cites W1979245931 @default.
• W4385647912 cites W1980666758 @default.
• W4385647912 cites W1987011468 @default.
• W4385647912 cites W1989167732 @default.
• W4385647912 cites W2000250108 @default.
• W4385647912 cites W2001326135 @default.
• W4385647912 cites W2002632741 @default.
• W4385647912 cites W2007373583 @default.
• W4385647912 cites W2009388543 @default.
• W4385647912 cites W2022577704 @default.
• W4385647912 cites W2024002899 @default.
• W4385647912 cites W2025078829 @default.
• W4385647912 cites W2031743305 @default.
• W4385647912 cites W2039440737 @default.
• W4385647912 cites W2044868519 @default.
• W4385647912 cites W2047106949 @default.
• W4385647912 cites W2049441082 @default.
• W4385647912 cites W2049752266 @default.
• W4385647912 cites W2052017885 @default.
• W4385647912 cites W2062771285 @default.
• W4385647912 cites W2068785669 @default.
• W4385647912 cites W2074486971 @default.
• W4385647912 cites W2079331869 @default.
• W4385647912 cites W2088095186 @default.
• W4385647912 cites W2091486071 @default.
• W4385647912 cites W2095807830 @default.
• W4385647912 cites W2099132633 @default.
• W4385647912 cites W2102431034 @default.
• W4385647912 cites W2104992826 @default.
• W4385647912 cites W2107277218 @default.
• W4385647912 cites W2111398277 @default.
• W4385647912 cites W2118476033 @default.
• W4385647912 cites W2126187754 @default.
• W4385647912 cites W2127049134 @default.
• W4385647912 cites W2142487687 @default.
• W4385647912 cites W2148821835 @default.
• W4385647912 cites W2192080449 @default.
• W4385647912 cites W2293300478 @default.
• W4385647912 cites W2418044989 @default.
• W4385647912 cites W2498477809 @default.
• W4385647912 cites W2562099055 @default.
• W4385647912 cites W2578491832 @default.
• W4385647912 cites W2603792353 @default.
• W4385647912 cites W2615917035 @default.
• W4385647912 cites W2617517460 @default.
• W4385647912 cites W2618947014 @default.
• W4385647912 cites W2759943938 @default.
• W4385647912 cites W2765280780 @default.
• W4385647912 cites W2802071782 @default.
• W4385647912 cites W2886785109 @default.
• W4385647912 cites W2940969167 @default.
• W4385647912 cites W2949836654 @default.
• W4385647912 cites W2951246313 @default.
• W4385647912 cites W2981987750 @default.
• W4385647912 cites W2995856956 @default.
• W4385647912 cites W2999616703 @default.
• W4385647912 cites W3089974198 @default.
• W4385647912 cites W3091894549 @default.
• W4385647912 cites W3134659922 @default.
• W4385647912 cites W3134719792 @default.
• W4385647912 cites W3153950346 @default.
• W4385647912 cites W3208125568 @default.
• W4385647912 cites W4243253843 @default.
• W4385647912 cites W4249526157 @default.
• W4385647912 cites W4281782537 @default.
• W4385647912 cites W4293253423 @default.
• W4385647912 cites W4378907976 @default.
• W4385647912 doi "https://doi.org/10.3389/fpls.2023.1208285" @default.
• W4385647912 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37615022" @default.
• W4385647912 hasPublicationYear "2023" @default.
• W4385647912 type Work @default.
• W4385647912 citedByCount "0" @default.
• W4385647912 crossrefType "journal-article" @default.
• W4385647912 hasAuthorship W4385647912A5012225970 @default.
• W4385647912 hasAuthorship W4385647912A5034719020 @default.
• W4385647912 hasAuthorship W4385647912A5045940717 @default.
• W4385647912 hasAuthorship W4385647912A5055268602 @default.
• W4385647912 hasAuthorship W4385647912A5057081627 @default.
• W4385647912 hasAuthorship W4385647912A5059670375 @default.
• W4385647912 hasBestOaLocation W43856479121 @default.
• W4385647912 hasConcept C104317684 @default.
• W4385647912 hasConcept C143065580 @default.
• W4385647912 hasConcept C148196450 @default.
• W4385647912 hasConcept C201437064 @default.
• W4385647912 hasConcept C2910992960 @default.

• next