Matches in SemOpenAlex for { <https://semopenalex.org/work/W4385655245> ?p ?o ?g. }
- W4385655245 abstract "Abstract Isoflavones are major specialized metabolites found in legume plants, where they contribute to environmental adaptation. Isoflavones also play a role human health as promising therapeutic agents. This metabolite group is involved in interactions with soil microorganisms as initiation signals in rhizobial symbiosis and as modulators of the legume root microbiota. We previously reported that isoflavones enrich the Comamonadaceae, a predominant bacterial family in soybean roots, and that microorganisms in legume rhizosphere soil degrade isoflavones. However, the isoflavone catabolism pathway that underly the isoflavone-mediated legume–microbiota interactions have not yet been clarified. Here, we isolated Variovorax sp. strain V35, member of the Comamonadaceae that harbors isoflavone-degrading activity, from soybean roots and discovered a gene cluster responsible for isoflavone degradation named ifc . Strain V35 metabolizes isoflavones in a completely distinct oxidative manner from the reductive isoflavone metabolism pathway elucidated in the gut microbiota, in which resulting products enter the tricarboxylic acid cycle. The characterization of ifc mutants and heterologously expressed IFC enzymes revealed that isoflavones are catabolized via A-ring cleaving fission, which starts with hydroxylation at the 8-position of the A-ring. We further demonstrated that ifc genes are frequently found in bacterial strains isolated from legume plants, including mutualistic rhizobia, and contribute to detoxification of the antibacterial activity of isoflavones. Taken together, our findings reveal an oxidative catabolism pathway of isoflavone in the soybean root microbiota, providing molecular insights into isoflavone-mediated legume–microbiota interactions. Significance Isoflavones play pivotal roles in plant-environment interactions and in the maintenance and improvement of human health. Bacterial metabolism is a fundamental component of isoflavone-mediated interkingdom interactions. In the human gut, intestinal bacteria convert isoflavones into equol, a highly bioactive compound. However, the fate of isoflavones in the legume rhizosphere has not been elucidated, despite them being the key signaling molecules for nodule symbiosis and modulation of the legume root microbiota. Here, we discovered a novel isoflavone catabolism pathway in the soybean root microbiota and demonstrated the strong association between bacterial catabolic abilities and their interactions with host plants. Collectively, our findings provide new insights into bacterial isoflavone metabolism and a molecular understanding of legume-microbiota interactions." @default.
- W4385655245 created "2023-08-09" @default.
- W4385655245 creator A5000796989 @default.
- W4385655245 creator A5030783451 @default.
- W4385655245 creator A5038913657 @default.
- W4385655245 creator A5040191814 @default.
- W4385655245 creator A5049901010 @default.
- W4385655245 creator A5051139328 @default.
- W4385655245 creator A5059144989 @default.
- W4385655245 creator A5061410563 @default.
- W4385655245 creator A5062442010 @default.
- W4385655245 creator A5065618135 @default.
- W4385655245 creator A5076458963 @default.
- W4385655245 creator A5086285278 @default.
- W4385655245 creator A5091297110 @default.
- W4385655245 date "2023-08-08" @default.
- W4385655245 modified "2023-09-25" @default.
- W4385655245 title "Discovery of an isoflavone oxidative catabolic pathway in legume root microbiota" @default.
- W4385655245 cites W123901329 @default.
- W4385655245 cites W1519002588 @default.
- W4385655245 cites W154734720 @default.
- W4385655245 cites W1627277677 @default.
- W4385655245 cites W1919626721 @default.
- W4385655245 cites W1963878289 @default.
- W4385655245 cites W1971852726 @default.
- W4385655245 cites W1987566169 @default.
- W4385655245 cites W2014749946 @default.
- W4385655245 cites W2015706279 @default.
- W4385655245 cites W2017640872 @default.
- W4385655245 cites W2027707636 @default.
- W4385655245 cites W2030985616 @default.
- W4385655245 cites W2035456956 @default.
- W4385655245 cites W2037490361 @default.
- W4385655245 cites W2047812682 @default.
- W4385655245 cites W2049424524 @default.
- W4385655245 cites W2064831811 @default.
- W4385655245 cites W2078336779 @default.
- W4385655245 cites W2096093282 @default.
- W4385655245 cites W2100596421 @default.
- W4385655245 cites W2116998966 @default.
- W4385655245 cites W2121905515 @default.
- W4385655245 cites W2128690877 @default.
- W4385655245 cites W2133400920 @default.
- W4385655245 cites W2136182806 @default.
- W4385655245 cites W2145998612 @default.
- W4385655245 cites W2151577590 @default.
- W4385655245 cites W2152395368 @default.
- W4385655245 cites W2294020897 @default.
- W4385655245 cites W2341951567 @default.
- W4385655245 cites W2550546313 @default.
- W4385655245 cites W2626587556 @default.
- W4385655245 cites W2766330607 @default.
- W4385655245 cites W2799721285 @default.
- W4385655245 cites W2839631923 @default.
- W4385655245 cites W2943667167 @default.
- W4385655245 cites W2944421815 @default.
- W4385655245 cites W2954019221 @default.
- W4385655245 cites W2982359249 @default.
- W4385655245 cites W2988396470 @default.
- W4385655245 cites W2997498046 @default.
- W4385655245 cites W3007960376 @default.
- W4385655245 cites W3098176099 @default.
- W4385655245 cites W3121652846 @default.
- W4385655245 cites W3131392146 @default.
- W4385655245 cites W3142350772 @default.
- W4385655245 cites W3165433555 @default.
- W4385655245 cites W3200012110 @default.
- W4385655245 cites W4220982507 @default.
- W4385655245 cites W4280550497 @default.
- W4385655245 cites W4306810394 @default.
- W4385655245 cites W4312065787 @default.
- W4385655245 cites W859509618 @default.
- W4385655245 doi "https://doi.org/10.1101/2023.08.07.552369" @default.
- W4385655245 hasPublicationYear "2023" @default.
- W4385655245 type Work @default.
- W4385655245 citedByCount "0" @default.
- W4385655245 crossrefType "posted-content" @default.
- W4385655245 hasAuthorship W4385655245A5000796989 @default.
- W4385655245 hasAuthorship W4385655245A5030783451 @default.
- W4385655245 hasAuthorship W4385655245A5038913657 @default.
- W4385655245 hasAuthorship W4385655245A5040191814 @default.
- W4385655245 hasAuthorship W4385655245A5049901010 @default.
- W4385655245 hasAuthorship W4385655245A5051139328 @default.
- W4385655245 hasAuthorship W4385655245A5059144989 @default.
- W4385655245 hasAuthorship W4385655245A5061410563 @default.
- W4385655245 hasAuthorship W4385655245A5062442010 @default.
- W4385655245 hasAuthorship W4385655245A5065618135 @default.
- W4385655245 hasAuthorship W4385655245A5076458963 @default.
- W4385655245 hasAuthorship W4385655245A5086285278 @default.
- W4385655245 hasAuthorship W4385655245A5091297110 @default.
- W4385655245 hasBestOaLocation W43856552451 @default.
- W4385655245 hasConcept C2779705811 @default.
- W4385655245 hasConcept C523546767 @default.
- W4385655245 hasConcept C54355233 @default.
- W4385655245 hasConcept C55493867 @default.
- W4385655245 hasConcept C86626682 @default.
- W4385655245 hasConcept C86803240 @default.
- W4385655245 hasConceptScore W4385655245C2779705811 @default.
- W4385655245 hasConceptScore W4385655245C523546767 @default.
- W4385655245 hasConceptScore W4385655245C54355233 @default.