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- W4385655789 abstract "Second Harmonic Generation (SHG) microscopy provides high resolution images of collagen-based structures, in particular ocular tissues. However, the implementation into in vivo conditions has been challenging. Here, we present a compact SHG microscope specifically developed to image the living human eye (cornea and sclera). The instrument was successfully employed to obtain non-contact and non-invasive SHG images within well-established light safety limits. Since SHG image quality is often limited by different physical factors (mainly at deeper locations), we also present some alternative methods to enhance the acquired images. These include a blind deconvolution approach and the use of incident radially polarized light. The effectiveness of a sub-10 fs used as illumination source is also analyzed." @default.
- W4385655789 created "2023-08-09" @default.
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- W4385655789 date "2023-07-02" @default.
- W4385655789 modified "2023-09-25" @default.
- W4385655789 title "Second Harmonic Generation Microscopy of the Living Human Eye: Limitations, Performance and Image Improvement" @default.
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- W4385655789 doi "https://doi.org/10.1109/icton59386.2023.10207248" @default.
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