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- W4385658736 abstract "Spermatogonial stem cells (SSCs) are essential for continuous spermatogenesis and male fertility. The underlying mechanisms of alternative splicing (AS) in mouse SSCs are still largely unclear. We demonstrated that SRSF1 is essential for gene expression and splicing in mouse SSCs. Crosslinking immunoprecipitation and sequencing (CLIP-seq) data revealed that spermatogonia-related genes (e.g., Plzf, Id4, Setdb1, Stra8, Tial1/Tiar, Bcas2, Ddx5, Srsf10, Uhrf1, and Bud31) were bound by SRSF1 in the mouse testes. Specific deletion of Srsf1 in mouse germ cells impairs homing and self-renewal of SSCs leading to male infertility. Whole-mount staining data showed the absence of germ cells in the testes of adult cKO mice, which indicates Sertoli cell-only syndrome (SCOS) in cKO mice. The expression of spermatogonia-related genes (Gfra1, Pou5f1, Plzf, Dnd1, Stra8, and Taf4b) was significantly reduced in the testes of conditional knockout (cKO) mice. Moreover, multiomics analysis suggests that SRSF1 directly binds and regulates the expression of Tial1/Tiar via AS to implement SSC homing and self-renewal. In addition, immunoprecipitation mass spectrometry (IP-MS) data showed that SRSF1 interacts with RNA splicing-related proteins (e.g., SRSF10, SART1, RBM15, SRRM2, SF3B6, and SF3A2). Collectively, our data reveal the critical role of SRSF1-mediated AS in SSC homing and self-renewal, which may provide a framework to elucidate the molecular mechanisms of the posttranscriptional network underlying the formation of SSC pools and the establishment of niches." @default.
- W4385658736 created "2023-08-09" @default.
- W4385658736 creator A5052408115 @default.
- W4385658736 date "2023-08-08" @default.
- W4385658736 modified "2023-10-16" @default.
- W4385658736 title "eLife assessment: SRSF1-mediated alternative splicing of Tial1/Tiar is essential for homing and self-renewal in mouse spermatogonial stem cells" @default.
- W4385658736 doi "https://doi.org/10.7554/elife.89316.1.sa0" @default.
- W4385658736 hasPublicationYear "2023" @default.
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