Matches in SemOpenAlex for { <https://semopenalex.org/work/W4385687555> ?p ?o ?g. }
Showing items 1 to 77 of
77
with 100 items per page.
- W4385687555 endingPage "224.02" @default.
- W4385687555 startingPage "224.02" @default.
- W4385687555 abstract "Abstract Mustela putorius furo uniquely reflects physiological and immunological changes in human aging such as immune imprinting from influenza infection, yet key ferret immunobiology knowledge gaps limit the scope of molecular analyses. First, ferret germline B cell receptor genes require identification. Additionally, the V, D, and J gene repertoire expressed by B cell receptors in antigen-experienced cells needs characterization. Illumina NGS was used to generate B-cell receptor mRNA data sets. A candidate list of gene segments was assembled including ferret genomic accessions homologous to known immunoglobulin genes and previously-known ferret immunoglobulin genes. NGS reads were queried against the candidate list using IgBLAST. IgBLAST hits were focused into consensus sequences using principal components analysis and K-means clustering. To identify IgG subclasses, putative ferret genome sequences sharing human IgG C genes were used to query Oxford Nanopore data that covered the FR3 through C gene area of transcripts. We report two new functional IGHV, two IGKV, and one IGLV gene segments in the ferret, with more sequences likely to surface in downstream analyses. We also corroborate most already-proposed functional V, D, and J gene segment sequences. Ferret orthologues to human IGHV subgroups commonly involved in stem-directed, broadly neutralizing antibody responses were identified. For the first time, productive IgG3 was described in the ferret model and distinguished from productive IgG1. Our report of novel ferret immunoglobulin genes will disinhibit the ferret as a model organism for the study of infectious disease. Specifically, it will inform the immunogenicity of candidate HA antigens in preclinical vaccine studies. Research reported in this abstract was supported by NIH NIAID contract 75N93019C00050." @default.
- W4385687555 created "2023-08-10" @default.
- W4385687555 creator A5022952869 @default.
- W4385687555 creator A5033890850 @default.
- W4385687555 creator A5041863425 @default.
- W4385687555 creator A5043980495 @default.
- W4385687555 creator A5046080905 @default.
- W4385687555 creator A5052629134 @default.
- W4385687555 creator A5064889731 @default.
- W4385687555 date "2023-05-01" @default.
- W4385687555 modified "2023-09-23" @default.
- W4385687555 title "Characterization of the B Cell Receptor Repertoire and Discovery of New Immunoglobulin Genes in the Domestic Ferret" @default.
- W4385687555 doi "https://doi.org/10.4049/jimmunol.210.supp.224.02" @default.
- W4385687555 hasPublicationYear "2023" @default.
- W4385687555 type Work @default.
- W4385687555 citedByCount "0" @default.
- W4385687555 crossrefType "journal-article" @default.
- W4385687555 hasAuthorship W4385687555A5022952869 @default.
- W4385687555 hasAuthorship W4385687555A5033890850 @default.
- W4385687555 hasAuthorship W4385687555A5041863425 @default.
- W4385687555 hasAuthorship W4385687555A5043980495 @default.
- W4385687555 hasAuthorship W4385687555A5046080905 @default.
- W4385687555 hasAuthorship W4385687555A5052629134 @default.
- W4385687555 hasAuthorship W4385687555A5064889731 @default.
- W4385687555 hasBestOaLocation W43856875551 @default.
- W4385687555 hasConcept C104317684 @default.
- W4385687555 hasConcept C128526571 @default.
- W4385687555 hasConcept C141231307 @default.
- W4385687555 hasConcept C159654299 @default.
- W4385687555 hasConcept C167625842 @default.
- W4385687555 hasConcept C2777609679 @default.
- W4385687555 hasConcept C2777938653 @default.
- W4385687555 hasConcept C2778453870 @default.
- W4385687555 hasConcept C2778461978 @default.
- W4385687555 hasConcept C3020196193 @default.
- W4385687555 hasConcept C54355233 @default.
- W4385687555 hasConcept C5911399 @default.
- W4385687555 hasConcept C70721500 @default.
- W4385687555 hasConcept C85630698 @default.
- W4385687555 hasConcept C86803240 @default.
- W4385687555 hasConcept C96007430 @default.
- W4385687555 hasConceptScore W4385687555C104317684 @default.
- W4385687555 hasConceptScore W4385687555C128526571 @default.
- W4385687555 hasConceptScore W4385687555C141231307 @default.
- W4385687555 hasConceptScore W4385687555C159654299 @default.
- W4385687555 hasConceptScore W4385687555C167625842 @default.
- W4385687555 hasConceptScore W4385687555C2777609679 @default.
- W4385687555 hasConceptScore W4385687555C2777938653 @default.
- W4385687555 hasConceptScore W4385687555C2778453870 @default.
- W4385687555 hasConceptScore W4385687555C2778461978 @default.
- W4385687555 hasConceptScore W4385687555C3020196193 @default.
- W4385687555 hasConceptScore W4385687555C54355233 @default.
- W4385687555 hasConceptScore W4385687555C5911399 @default.
- W4385687555 hasConceptScore W4385687555C70721500 @default.
- W4385687555 hasConceptScore W4385687555C85630698 @default.
- W4385687555 hasConceptScore W4385687555C86803240 @default.
- W4385687555 hasConceptScore W4385687555C96007430 @default.
- W4385687555 hasIssue "1_Supplement" @default.
- W4385687555 hasLocation W43856875551 @default.
- W4385687555 hasOpenAccess W4385687555 @default.
- W4385687555 hasPrimaryLocation W43856875551 @default.
- W4385687555 hasRelatedWork W1852202120 @default.
- W4385687555 hasRelatedWork W2062581231 @default.
- W4385687555 hasRelatedWork W2069184423 @default.
- W4385687555 hasRelatedWork W2565469387 @default.
- W4385687555 hasRelatedWork W2758988497 @default.
- W4385687555 hasRelatedWork W2986268929 @default.
- W4385687555 hasRelatedWork W3027443406 @default.
- W4385687555 hasRelatedWork W3092193830 @default.
- W4385687555 hasRelatedWork W4236284589 @default.
- W4385687555 hasRelatedWork W4386024603 @default.
- W4385687555 hasVolume "210" @default.
- W4385687555 isParatext "false" @default.
- W4385687555 isRetracted "false" @default.
- W4385687555 workType "article" @default.