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- W4385705637 abstract "Great strides in the field of lipidomics driven by advances in mass spectrometry techniques in the last decade have moved lipid analysis to a new level and significantly improved our understanding of lipid biochemistry. Multiple stage mass spectrometry (MSn) with high resolution mass spectrometry (HRMS) that allows sequential isolation, fragmentation, and recognition of ion structures, is a powerful tool for characterization of complex and diversified lipid in bacterial cells, in which lipids are often critical for cell aggregation and dissociation, and play important biological roles. In addition to common phospholipids, many bacteria contain unique lipids that are specific to the bacterium genus and even to the bacterium species. In this review, application of linear ion-trap (LIT) MSn in the structural characterization of native bacterial lipids including (1) novel lipids consisting of many isomeric structures, (2) lipids with unique functional groups and modification, (3) complex sphingolipids, peptidolipids, and lipocyclopeptides from various bacteria are presented. LIT MSn approach affords realization of the mechanisms underlying the fragmentation processes, resulting in identification of complex lipid structures that would be very difficult to define using other analytical methods." @default.
- W4385705637 created "2023-08-10" @default.
- W4385705637 creator A5038207517 @default.
- W4385705637 date "2023-08-01" @default.
- W4385705637 modified "2023-10-15" @default.
- W4385705637 title "Multiple stage linear ion-trap mass spectrometry toward characterization of native bacterial lipids-a critical review" @default.
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- W4385705637 doi "https://doi.org/10.1016/j.biochi.2023.08.009" @default.
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