Matches in SemOpenAlex for { <https://semopenalex.org/work/W4385718834> ?p ?o ?g. }
Showing items 1 to 85 of
85
with 100 items per page.
- W4385718834 abstract "Abstract Iron-sulfur (Fe-S) proteins are essential for the ability of methanogens to carry out methanogenesis and biological nitrogen fixation (diazotrophy). Nonetheless, the factors involved in Fe-S cluster biogenesis in methanogens remain largely unknown. The minimal SUF Fe-S cluster biogenesis system (i.e., SufBC) is postulated to serve as the primary system in methanogens. Here, the role of SufBC in Methanosarcina acetivorans , which contains two sufCB gene clusters, was investigated. The CRISPRi-dCas9 and CRISPR-Cas9 systems were utilized to repress or delete sufC1B1 and sufC2B2 , respectively. Neither the dual repression of sufC1B1 and sufC2B2 nor the deletion of both sufC1B1 and sufC2B2 affected the growth of M. acetivorans under any conditions tested, including diazotrophy. Interestingly, deletion of only sufC1B1 led to a delayed-growth phenotype under all growth conditions, suggesting that the deletion of sufC2B2 acts as a suppressor mutation in the absence of sufC1B1 . In addition, the deletion of sufC1B1 and/or sufC2B2 did not affect the total Fe-S cluster content in M. acetivorans cells. Overall, these results reveal that the minimal SUF system is not required for Fe-S cluster biogenesis in M. acetivorans and challenge the universal role of SufBC in Fe-S cluster biogenesis in methanogens." @default.
- W4385718834 created "2023-08-11" @default.
- W4385718834 creator A5044088562 @default.
- W4385718834 creator A5052630022 @default.
- W4385718834 creator A5066959230 @default.
- W4385718834 date "2023-08-10" @default.
- W4385718834 modified "2023-09-27" @default.
- W4385718834 title "The minimal SUF system is not required for Fe-S cluster biogenesis in the methanogenic archaeon Methanosarcina acetivorans" @default.
- W4385718834 cites W1550904083 @default.
- W4385718834 cites W1565461456 @default.
- W4385718834 cites W1971891612 @default.
- W4385718834 cites W2019519199 @default.
- W4385718834 cites W2022897538 @default.
- W4385718834 cites W2046719899 @default.
- W4385718834 cites W2047304632 @default.
- W4385718834 cites W2066510550 @default.
- W4385718834 cites W2074905007 @default.
- W4385718834 cites W2075040966 @default.
- W4385718834 cites W2080658804 @default.
- W4385718834 cites W2097062730 @default.
- W4385718834 cites W2098953264 @default.
- W4385718834 cites W2105662163 @default.
- W4385718834 cites W2110722213 @default.
- W4385718834 cites W2115806782 @default.
- W4385718834 cites W2116472408 @default.
- W4385718834 cites W2133730287 @default.
- W4385718834 cites W2155205959 @default.
- W4385718834 cites W2157433322 @default.
- W4385718834 cites W2162863397 @default.
- W4385718834 cites W2163917615 @default.
- W4385718834 cites W2215633395 @default.
- W4385718834 cites W2527687534 @default.
- W4385718834 cites W2581262905 @default.
- W4385718834 cites W2592448201 @default.
- W4385718834 cites W2746754871 @default.
- W4385718834 cites W2778862248 @default.
- W4385718834 cites W2981269603 @default.
- W4385718834 cites W3002794944 @default.
- W4385718834 cites W3093686911 @default.
- W4385718834 cites W3166941994 @default.
- W4385718834 cites W3168728356 @default.
- W4385718834 cites W3179494712 @default.
- W4385718834 cites W4293247451 @default.
- W4385718834 cites W4296037365 @default.
- W4385718834 doi "https://doi.org/10.21203/rs.3.rs-3232272/v1" @default.
- W4385718834 hasPublicationYear "2023" @default.
- W4385718834 type Work @default.
- W4385718834 citedByCount "0" @default.
- W4385718834 crossrefType "posted-content" @default.
- W4385718834 hasAuthorship W4385718834A5044088562 @default.
- W4385718834 hasAuthorship W4385718834A5052630022 @default.
- W4385718834 hasAuthorship W4385718834A5066959230 @default.
- W4385718834 hasBestOaLocation W43857188341 @default.
- W4385718834 hasConcept C104317684 @default.
- W4385718834 hasConcept C131934819 @default.
- W4385718834 hasConcept C2778563749 @default.
- W4385718834 hasConcept C2778589620 @default.
- W4385718834 hasConcept C523546767 @default.
- W4385718834 hasConcept C54355233 @default.
- W4385718834 hasConcept C550995028 @default.
- W4385718834 hasConcept C86803240 @default.
- W4385718834 hasConceptScore W4385718834C104317684 @default.
- W4385718834 hasConceptScore W4385718834C131934819 @default.
- W4385718834 hasConceptScore W4385718834C2778563749 @default.
- W4385718834 hasConceptScore W4385718834C2778589620 @default.
- W4385718834 hasConceptScore W4385718834C523546767 @default.
- W4385718834 hasConceptScore W4385718834C54355233 @default.
- W4385718834 hasConceptScore W4385718834C550995028 @default.
- W4385718834 hasConceptScore W4385718834C86803240 @default.
- W4385718834 hasLocation W43857188341 @default.
- W4385718834 hasOpenAccess W4385718834 @default.
- W4385718834 hasPrimaryLocation W43857188341 @default.
- W4385718834 hasRelatedWork W161010695 @default.
- W4385718834 hasRelatedWork W1964872012 @default.
- W4385718834 hasRelatedWork W201238788 @default.
- W4385718834 hasRelatedWork W2112126698 @default.
- W4385718834 hasRelatedWork W2796419202 @default.
- W4385718834 hasRelatedWork W3126204815 @default.
- W4385718834 hasRelatedWork W4210408867 @default.
- W4385718834 hasRelatedWork W4229446195 @default.
- W4385718834 hasRelatedWork W4385718834 @default.
- W4385718834 hasRelatedWork W4386723141 @default.
- W4385718834 isParatext "false" @default.
- W4385718834 isRetracted "false" @default.
- W4385718834 workType "article" @default.