FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4385790111> ?p ?o ?g. }

• W4385790111 endingPage "76" @default.
• W4385790111 startingPage "55" @default.
• W4385790111 abstract "Eukaryotic DNA exists in chromatin, where the genomic DNA is packaged into a fundamental repeating unit known as the nucleosome. In this chromatin environment, our genomic DNA is constantly under attack by exogenous and endogenous stressors that can lead to DNA damage. Importantly, this DNA damage must be repaired to prevent the accumulation of mutations and ensure normal cellular function. To date, most in-depth biochemical studies of DNA repair proteins have been performed in the context of free duplex DNA. However, chromatin can serve as a barrier that DNA repair enzymes must navigate in order find, access, and process DNA damage in the cell. To facilitate future studies of DNA repair in chromatin, we describe a protocol for generating nucleosome containing site-specific DNA damage that can be utilized for a variety of in vitro applications. This protocol describes several key steps including how to generate damaged DNA oligonucleotides, the expression and purification of recombinant histones, the refolding of histone complexes, and the reconstitution of nucleosomes containing site-specific DNA damage. These methods will enable researchers to generate nucleosomes containing site-specific DNA damage for extensive biochemical and structural studies of DNA repair in the nucleosome." @default.
• W4385790111 created "2023-08-13" @default.
• W4385790111 creator A5001621167 @default.
• W4385790111 creator A5042463108 @default.
• W4385790111 creator A5053552465 @default.
• W4385790111 creator A5064369961 @default.
• W4385790111 date "2023-01-01" @default.
• W4385790111 modified "2023-10-14" @default.
• W4385790111 title "Generation of Recombinant Nucleosomes Containing Site-Specific DNA Damage" @default.
• W4385790111 cites W13874100 @default.
• W4385790111 cites W1507013865 @default.
• W4385790111 cites W1571482483 @default.
• W4385790111 cites W1787312915 @default.
• W4385790111 cites W1862617947 @default.
• W4385790111 cites W1966691006 @default.
• W4385790111 cites W1967990398 @default.
• W4385790111 cites W1979020998 @default.
• W4385790111 cites W1981565987 @default.
• W4385790111 cites W1985323804 @default.
• W4385790111 cites W1986364710 @default.
• W4385790111 cites W1986914420 @default.
• W4385790111 cites W1988997227 @default.
• W4385790111 cites W1997621038 @default.
• W4385790111 cites W1998028181 @default.
• W4385790111 cites W2010339813 @default.
• W4385790111 cites W2011523054 @default.
• W4385790111 cites W2013571070 @default.
• W4385790111 cites W2016844537 @default.
• W4385790111 cites W2019747371 @default.
• W4385790111 cites W2030475136 @default.
• W4385790111 cites W2034591093 @default.
• W4385790111 cites W2038234852 @default.
• W4385790111 cites W2038840504 @default.
• W4385790111 cites W2044666776 @default.
• W4385790111 cites W2052650166 @default.
• W4385790111 cites W2061399364 @default.
• W4385790111 cites W2062208582 @default.
• W4385790111 cites W2068533511 @default.
• W4385790111 cites W2071748710 @default.
• W4385790111 cites W2076316122 @default.
• W4385790111 cites W2082586163 @default.
• W4385790111 cites W2084695608 @default.
• W4385790111 cites W2124106910 @default.
• W4385790111 cites W2127673921 @default.
• W4385790111 cites W2128135812 @default.
• W4385790111 cites W2131415510 @default.
• W4385790111 cites W2140126848 @default.
• W4385790111 cites W2142592261 @default.
• W4385790111 cites W2144792857 @default.
• W4385790111 cites W2154111088 @default.
• W4385790111 cites W2157089275 @default.
• W4385790111 cites W2322175438 @default.
• W4385790111 cites W2461153362 @default.
• W4385790111 cites W2542466015 @default.
• W4385790111 cites W2578182948 @default.
• W4385790111 cites W2580397696 @default.
• W4385790111 cites W2587005527 @default.
• W4385790111 cites W2587980633 @default.
• W4385790111 cites W2588394019 @default.
• W4385790111 cites W2726583977 @default.
• W4385790111 cites W2735338880 @default.
• W4385790111 cites W2736809432 @default.
• W4385790111 cites W2750976270 @default.
• W4385790111 cites W2767401773 @default.
• W4385790111 cites W2782571697 @default.
• W4385790111 cites W2794848326 @default.
• W4385790111 cites W2805178071 @default.
• W4385790111 cites W2885472321 @default.
• W4385790111 cites W2888380493 @default.
• W4385790111 cites W2890191449 @default.
• W4385790111 cites W2900376084 @default.
• W4385790111 cites W2902508124 @default.
• W4385790111 cites W2909527114 @default.
• W4385790111 cites W2941280330 @default.
• W4385790111 cites W2949254762 @default.
• W4385790111 cites W2960761486 @default.
• W4385790111 cites W2966933138 @default.
• W4385790111 cites W2968340746 @default.
• W4385790111 cites W2981066584 @default.
• W4385790111 cites W2988152034 @default.
• W4385790111 cites W2994032997 @default.
• W4385790111 cites W3016105018 @default.
• W4385790111 cites W3022838746 @default.
• W4385790111 cites W3090573334 @default.
• W4385790111 cites W3112542908 @default.
• W4385790111 cites W3119438165 @default.
• W4385790111 cites W3126342449 @default.
• W4385790111 cites W3135024668 @default.
• W4385790111 cites W4295679026 @default.
• W4385790111 doi "https://doi.org/10.1007/978-1-0716-3373-1_4" @default.
• W4385790111 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37574475" @default.
• W4385790111 hasPublicationYear "2023" @default.
• W4385790111 type Work @default.
• W4385790111 citedByCount "0" @default.
• W4385790111 crossrefType "book-chapter" @default.
• W4385790111 hasAuthorship W4385790111A5001621167 @default.
• W4385790111 hasAuthorship W4385790111A5042463108 @default.
• W4385790111 hasAuthorship W4385790111A5053552465 @default.

• next