Matches in SemOpenAlex for { <https://semopenalex.org/work/W4385794259> ?p ?o ?g. }
- W4385794259 abstract "Abstract A hallmark of α-synucleinopathies including Parkinson’s disease, dementia with Lewy bodies, and multiple system atrophy, is the misfolding and aggregation of α-synuclein in tissues and biological fluids. Real-time quaking-induced conversion (RT-QuIC) is a protein amplification assay providing ultrasensitive detection of disease-associated misfolded proteins yet is currently limited to invasive sample types such as cerebrospinal fluid for clinical use. More accessible sample types, especially blood, contain inhibitors that interfere with the RT-QuIC assay. Here, we show that Nanoparticle-enhanced Quaking-induced Conversion (Nano-QuIC) can greatly accelerate the detection and improves sensitivity of detection of misfolded alpha-synuclein spiked into extremely complex samples such as human plasma and whole lysed bovine blood compared to RT-QuIC. In spiked human plasma samples, Nano-QuIC showed 100-fold sensitivity improvement while doubling the speed of the reaction. In lysed whole bovine blood, Nano-QuIC detected concentrations of misfolded α-synuclein down to 90 pg/ml while RT-QuIC failed to have any detection due to the presence of strong inhibitors. Crucially, no false positives were observed in human plasma or lysed whole blood with 50 nm silica nanoparticles. This sets the groundwork for noninvasive diagnostic use of Nano-QuIC toward enabling early disease detection and management through blood-based testing. TOC graphic" @default.
- W4385794259 created "2023-08-14" @default.
- W4385794259 creator A5004487444 @default.
- W4385794259 creator A5012515985 @default.
- W4385794259 creator A5026725025 @default.
- W4385794259 creator A5030460272 @default.
- W4385794259 creator A5032107505 @default.
- W4385794259 creator A5041132643 @default.
- W4385794259 creator A5078393968 @default.
- W4385794259 creator A5085640730 @default.
- W4385794259 date "2023-08-13" @default.
- W4385794259 modified "2023-10-01" @default.
- W4385794259 title "Blood-based Nano-QuIC: Accelerated and Inhibitor-resistant Detection of Misfolded α-synuclein" @default.
- W4385794259 cites W1527671059 @default.
- W4385794259 cites W1837152578 @default.
- W4385794259 cites W1979591162 @default.
- W4385794259 cites W2010477422 @default.
- W4385794259 cites W2014135584 @default.
- W4385794259 cites W2022252153 @default.
- W4385794259 cites W2033904939 @default.
- W4385794259 cites W2038342005 @default.
- W4385794259 cites W2052004580 @default.
- W4385794259 cites W2063623360 @default.
- W4385794259 cites W2157102136 @default.
- W4385794259 cites W2513452411 @default.
- W4385794259 cites W2520893942 @default.
- W4385794259 cites W2531211039 @default.
- W4385794259 cites W2554758214 @default.
- W4385794259 cites W2567163077 @default.
- W4385794259 cites W2604665474 @default.
- W4385794259 cites W2785526679 @default.
- W4385794259 cites W2802957172 @default.
- W4385794259 cites W2809410660 @default.
- W4385794259 cites W2892069682 @default.
- W4385794259 cites W2914135810 @default.
- W4385794259 cites W2940371405 @default.
- W4385794259 cites W2966520883 @default.
- W4385794259 cites W2990451427 @default.
- W4385794259 cites W2993954718 @default.
- W4385794259 cites W2994146441 @default.
- W4385794259 cites W3005252823 @default.
- W4385794259 cites W3036098943 @default.
- W4385794259 cites W3045011437 @default.
- W4385794259 cites W3087080230 @default.
- W4385794259 cites W3129008573 @default.
- W4385794259 cites W3133514358 @default.
- W4385794259 cites W3148543271 @default.
- W4385794259 cites W3153322458 @default.
- W4385794259 cites W3194729933 @default.
- W4385794259 cites W3214409719 @default.
- W4385794259 cites W4205421335 @default.
- W4385794259 cites W4220745743 @default.
- W4385794259 cites W4223948668 @default.
- W4385794259 cites W4235002974 @default.
- W4385794259 cites W4283394158 @default.
- W4385794259 cites W4285726946 @default.
- W4385794259 cites W4288084637 @default.
- W4385794259 cites W4317242213 @default.
- W4385794259 cites W4365459497 @default.
- W4385794259 cites W4367049330 @default.
- W4385794259 cites W4367856929 @default.
- W4385794259 cites W4378637394 @default.
- W4385794259 doi "https://doi.org/10.1101/2023.08.09.552630" @default.
- W4385794259 hasPublicationYear "2023" @default.
- W4385794259 type Work @default.
- W4385794259 citedByCount "0" @default.
- W4385794259 crossrefType "posted-content" @default.
- W4385794259 hasAuthorship W4385794259A5004487444 @default.
- W4385794259 hasAuthorship W4385794259A5012515985 @default.
- W4385794259 hasAuthorship W4385794259A5026725025 @default.
- W4385794259 hasAuthorship W4385794259A5030460272 @default.
- W4385794259 hasAuthorship W4385794259A5032107505 @default.
- W4385794259 hasAuthorship W4385794259A5041132643 @default.
- W4385794259 hasAuthorship W4385794259A5078393968 @default.
- W4385794259 hasAuthorship W4385794259A5085640730 @default.
- W4385794259 hasBestOaLocation W43857942591 @default.
- W4385794259 hasConcept C142724271 @default.
- W4385794259 hasConcept C153911025 @default.
- W4385794259 hasConcept C185592680 @default.
- W4385794259 hasConcept C2777440831 @default.
- W4385794259 hasConcept C2778548049 @default.
- W4385794259 hasConcept C2779134260 @default.
- W4385794259 hasConcept C2779483572 @default.
- W4385794259 hasConcept C2779734285 @default.
- W4385794259 hasConcept C2781449126 @default.
- W4385794259 hasConcept C55493867 @default.
- W4385794259 hasConcept C57409179 @default.
- W4385794259 hasConcept C71924100 @default.
- W4385794259 hasConcept C86803240 @default.
- W4385794259 hasConceptScore W4385794259C142724271 @default.
- W4385794259 hasConceptScore W4385794259C153911025 @default.
- W4385794259 hasConceptScore W4385794259C185592680 @default.
- W4385794259 hasConceptScore W4385794259C2777440831 @default.
- W4385794259 hasConceptScore W4385794259C2778548049 @default.
- W4385794259 hasConceptScore W4385794259C2779134260 @default.
- W4385794259 hasConceptScore W4385794259C2779483572 @default.
- W4385794259 hasConceptScore W4385794259C2779734285 @default.
- W4385794259 hasConceptScore W4385794259C2781449126 @default.
- W4385794259 hasConceptScore W4385794259C55493867 @default.
- W4385794259 hasConceptScore W4385794259C57409179 @default.