FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4385804302> ?p ?o ?g. }

• W4385804302 endingPage "117048" @default.
• W4385804302 startingPage "117048" @default.
• W4385804302 abstract "Corydalis bungeana Turcz. (KDD) is a Chinese herbal medicine with anti-inflammatory, lung cleansing, detoxification and other functions. Clinically, it is commonly used to treat respiratory infections. This study uses ALI as the research model, which is consistent with the clinical use of KDD. Acetylcorynoline (AC) is the main alkaloid component of the KDD extracts, and network pharmacology studies suggest that it may be the main active ingredient in the prevention of ALI.The aim of this study is to explore the underlying mechanisms and to study the efficacy material basis of KDD in anti-ALI effect by LPS-induced mice and using a combination of RNA sequencing (RNA-Seq) technology and network pharmacology.Establish a mouse model of ALI by intraperitoneal injection of LPS (5 mg/kg). The main active ingredients of KDD were identified and analyzed by high performance liquid chromatography with quadrupole time-of-flight mass spectrometry (HPLC-QTOF-MS) and network pharmacology. IL-18, IL-1β, and IL-6 levels in serum and bronchoalveolar lavage fluid (BALF), lung histopathological changes, and lung myeloperoxidase (MPO) activity were assessed. We investigated the possible molecular mechanisms of KDD and AC in an LPS-induced mouse ALI models with RNA-Seq technology. In addition, the anti-inflammatory effect of AC was verified in vitro by establishing an LPS-stimulated RAW264.7 inflammation model. Molecular docking further validated AC as the efficacy material basis of KDD in anti-ALI.Based on HPLC-QTOF-MS technology and network pharmacology, KDD is more strongly associated with lung tissue, and that AC may be the main active ingredient of KDD. Subsequently, in vivo experiments results showed that KDD and AC reduced the levels of pro-inflammatory cytokines in serum and BALF, reduced MPO levels and reduced inflammatory damage in the lungs. To elucidate its underlying mechanism, based on RNA-Seq analysis techniques performed in lung tissue, enrichment analysis showed that KDD and AC intervened through the NLR signaling pathway, thereby mitigating LPS-induced ALI. Then, RT-qPCR, IF, WB and other technologies were used to verify the anti-ALI core difference genes of KDD and AC from the gene transcription and protein expression levels of the NLR signaling pathway, and confirmed the anti-ALI. In vitro experimental results also showed that AC has anti-inflammatory effects in RAW264.7. Finally, the biotransformation and molecular docking results also further indicated that AC is the active ingredient of KDD in anti-ALI.Studies have shown that KDD has a good therapeutic effect on ALI, and AC is the main pharmacodynamic material basis for its therapeutic effect in ALI." @default.
• W4385804302 created "2023-08-15" @default.
• W4385804302 creator A5041093814 @default.
• W4385804302 creator A5044903224 @default.
• W4385804302 creator A5051257569 @default.
• W4385804302 creator A5060173991 @default.
• W4385804302 creator A5062309027 @default.
• W4385804302 creator A5075877965 @default.
• W4385804302 creator A5077314347 @default.
• W4385804302 creator A5084440288 @default.
• W4385804302 date "2024-01-01" @default.
• W4385804302 modified "2023-10-14" @default.
• W4385804302 title "An integrated RNA-Seq and network pharmacology approach for exploring the preventive effect of Corydalis bungeana Turcz. Extract and Acetylcorynoline on LPS-induced acute lung injury" @default.
• W4385804302 cites W1934746052 @default.
• W4385804302 cites W1989186591 @default.
• W4385804302 cites W2005410446 @default.
• W4385804302 cites W2009313526 @default.
• W4385804302 cites W2130410032 @default.
• W4385804302 cites W2133813119 @default.
• W4385804302 cites W2162151166 @default.
• W4385804302 cites W2169267616 @default.
• W4385804302 cites W2178922593 @default.
• W4385804302 cites W2201316930 @default.
• W4385804302 cites W2275708639 @default.
• W4385804302 cites W2317488575 @default.
• W4385804302 cites W2431987552 @default.
• W4385804302 cites W2476155396 @default.
• W4385804302 cites W2518732602 @default.
• W4385804302 cites W2793513555 @default.
• W4385804302 cites W2946412700 @default.
• W4385804302 cites W2987928072 @default.
• W4385804302 cites W2988227719 @default.
• W4385804302 cites W3010959161 @default.
• W4385804302 cites W3084230788 @default.
• W4385804302 cites W3101152033 @default.
• W4385804302 cites W3110500961 @default.
• W4385804302 cites W3134240192 @default.
• W4385804302 cites W3176543669 @default.
• W4385804302 cites W4211250103 @default.
• W4385804302 cites W4226271342 @default.
• W4385804302 cites W4285496138 @default.
• W4385804302 cites W4292572953 @default.
• W4385804302 doi "https://doi.org/10.1016/j.jep.2023.117048" @default.
• W4385804302 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37586441" @default.
• W4385804302 hasPublicationYear "2024" @default.
• W4385804302 type Work @default.
• W4385804302 citedByCount "0" @default.
• W4385804302 crossrefType "journal-article" @default.
• W4385804302 hasAuthorship W4385804302A5041093814 @default.
• W4385804302 hasAuthorship W4385804302A5044903224 @default.
• W4385804302 hasAuthorship W4385804302A5051257569 @default.
• W4385804302 hasAuthorship W4385804302A5060173991 @default.
• W4385804302 hasAuthorship W4385804302A5062309027 @default.
• W4385804302 hasAuthorship W4385804302A5075877965 @default.
• W4385804302 hasAuthorship W4385804302A5077314347 @default.
• W4385804302 hasAuthorship W4385804302A5084440288 @default.
• W4385804302 hasConcept C126322002 @default.
• W4385804302 hasConcept C142724271 @default.
• W4385804302 hasConcept C188947578 @default.
• W4385804302 hasConcept C204787440 @default.
• W4385804302 hasConcept C2776190711 @default.
• W4385804302 hasConcept C2777714996 @default.
• W4385804302 hasConcept C2777961210 @default.
• W4385804302 hasConcept C2778978776 @default.
• W4385804302 hasConcept C2780035454 @default.
• W4385804302 hasConcept C45904493 @default.
• W4385804302 hasConcept C556039675 @default.
• W4385804302 hasConcept C71924100 @default.
• W4385804302 hasConcept C98274493 @default.
• W4385804302 hasConceptScore W4385804302C126322002 @default.
• W4385804302 hasConceptScore W4385804302C142724271 @default.
• W4385804302 hasConceptScore W4385804302C188947578 @default.
• W4385804302 hasConceptScore W4385804302C204787440 @default.
• W4385804302 hasConceptScore W4385804302C2776190711 @default.
• W4385804302 hasConceptScore W4385804302C2777714996 @default.
• W4385804302 hasConceptScore W4385804302C2777961210 @default.
• W4385804302 hasConceptScore W4385804302C2778978776 @default.
• W4385804302 hasConceptScore W4385804302C2780035454 @default.
• W4385804302 hasConceptScore W4385804302C45904493 @default.
• W4385804302 hasConceptScore W4385804302C556039675 @default.
• W4385804302 hasConceptScore W4385804302C71924100 @default.
• W4385804302 hasConceptScore W4385804302C98274493 @default.
• W4385804302 hasLocation W43858043021 @default.
• W4385804302 hasLocation W43858043022 @default.
• W4385804302 hasOpenAccess W4385804302 @default.
• W4385804302 hasPrimaryLocation W43858043021 @default.
• W4385804302 hasRelatedWork W2315787763 @default.
• W4385804302 hasRelatedWork W2362206677 @default.
• W4385804302 hasRelatedWork W2379434545 @default.
• W4385804302 hasRelatedWork W2412968296 @default.
• W4385804302 hasRelatedWork W2434292116 @default.
• W4385804302 hasRelatedWork W3030759040 @default.
• W4385804302 hasRelatedWork W4200419177 @default.
• W4385804302 hasRelatedWork W4246250287 @default.
• W4385804302 hasRelatedWork W4319317039 @default.
• W4385804302 hasRelatedWork W820636411 @default.
• W4385804302 hasVolume "318" @default.
• W4385804302 isParatext "false" @default.

• next