FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4385812571> ?p ?o ?g. }

• W4385812571 abstract "Abstract Background The immune system plays a crucial role in initiating, progressing, and disseminating HNSCC. This study aims to investigate the differences in immune microenvironments between 2D-4-culture and 3D-4-culture models of head and neck squamous cell carcinoma (HNSCC) cells (FaDu), human fibroblasts (HF), human monocytes (THP-1), and human endothelial cells (HUVEC). Methods For the 3D-4-culture model, FaDu:HF: THP-1 (2:1:1) was inoculated in an ultra-low attachment culture plate, while HUVEC was placed in a transwell chamber. The ordinary culture plate was used for the 2D-4-culture model. Tumor-associated macrophage markers (CD163), tumor-associated fibroblast markers (FAP), and epithelial-mesenchymal transition (EMT) were detected by western blot. Inflammatory cytokines (IL-4, IL-2, CXCL 10, IL-1 β, TNF-α, CCL 2, IL-17 A, IL-6, IL-10, IFN-γ, IL-12 p 70, CXCL 8, TGFβ1) in the supernatant were measured by flow cytometry. HUVEC migration was observed under a microscope. The 3D spheres were stained and observed with a confocal microscope. CCK8 assay was used to detect the resistance of mixed cells to cisplatin in both 2D-4-culture and 3D-4-culture. Results After three days of co-culture, the 3D-4-culture model showed increased expression levels of CD163 and FAP proteins (both P < 0.001), increased expression of E-cadherin protein and N-cadherin protein expression (P < 0.001), decreased expression of vimentin (P < 0.01) and Twist protein (P < 0.001). HUVEC migration significantly increased (P < 0.001), as did the concentrations of IP-10, MCP-1, IL-6, and IL-10 (all P < 0.001). Confocal microscopy showed that 3D-4 co-culture formed loose cell clusters on day 1, which gradually became a dense sphere surrounded by FaDu cells invading the inside. After co-culturing for 24h, 48h, and 72h, the resistance of mix cells to cisplatin in 3D-4-culture was significantly higher than in 2D-4-culture (P < 0.01 for all). Conclusion Compared to 2D-4-culture, 3D-4-culture better simulates the in vivo immune microenvironment of HNSCC by promoting fibroblast transformation into tumor-associated fibroblasts, monocyte transformation into tumor-associated macrophages, enhancing endothelial cell migration ability, partial EMT formation in HNSCC cells, and is more suitable for studying the immunosuppressive microenvironment of HNSCC." @default.
• W4385812571 created "2023-08-15" @default.
• W4385812571 creator A5013493157 @default.
• W4385812571 creator A5025198232 @default.
• W4385812571 creator A5033356745 @default.
• W4385812571 creator A5059957140 @default.
• W4385812571 creator A5067962033 @default.
• W4385812571 creator A5076385283 @default.
• W4385812571 creator A5089245987 @default.
• W4385812571 date "2023-08-14" @default.
• W4385812571 modified "2023-10-02" @default.
• W4385812571 title "Changes of immune microenvironment in head and neck squamous cell carcinoma in 3D-4-culture compared to 2D-4-culture" @default.
• W4385812571 cites W1896060797 @default.
• W4385812571 cites W2004046483 @default.
• W4385812571 cites W2026548544 @default.
• W4385812571 cites W2036959910 @default.
• W4385812571 cites W2042198997 @default.
• W4385812571 cites W2059918221 @default.
• W4385812571 cites W2061385235 @default.
• W4385812571 cites W2084904115 @default.
• W4385812571 cites W2090177390 @default.
• W4385812571 cites W2104313815 @default.
• W4385812571 cites W2110567472 @default.
• W4385812571 cites W2110664940 @default.
• W4385812571 cites W2111442242 @default.
• W4385812571 cites W2163814058 @default.
• W4385812571 cites W2165316896 @default.
• W4385812571 cites W2190528488 @default.
• W4385812571 cites W2475501791 @default.
• W4385812571 cites W2520107693 @default.
• W4385812571 cites W2524198677 @default.
• W4385812571 cites W2529484692 @default.
• W4385812571 cites W2555216255 @default.
• W4385812571 cites W2582686270 @default.
• W4385812571 cites W2612999759 @default.
• W4385812571 cites W2734862811 @default.
• W4385812571 cites W2749613778 @default.
• W4385812571 cites W2766225245 @default.
• W4385812571 cites W2788371883 @default.
• W4385812571 cites W2886833531 @default.
• W4385812571 cites W2888803951 @default.
• W4385812571 cites W2897128051 @default.
• W4385812571 cites W2913684383 @default.
• W4385812571 cites W2924003728 @default.
• W4385812571 cites W2938964227 @default.
• W4385812571 cites W2950591923 @default.
• W4385812571 cites W3009670898 @default.
• W4385812571 cites W3012894904 @default.
• W4385812571 cites W3048942453 @default.
• W4385812571 cites W3107533236 @default.
• W4385812571 cites W3120421709 @default.
• W4385812571 cites W3127697076 @default.
• W4385812571 cites W3128646645 @default.
• W4385812571 cites W3163582350 @default.
• W4385812571 cites W3163840476 @default.
• W4385812571 doi "https://doi.org/10.21203/rs.3.rs-3179524/v1" @default.
• W4385812571 hasPublicationYear "2023" @default.
• W4385812571 type Work @default.
• W4385812571 citedByCount "0" @default.
• W4385812571 crossrefType "posted-content" @default.
• W4385812571 hasAuthorship W4385812571A5013493157 @default.
• W4385812571 hasAuthorship W4385812571A5025198232 @default.
• W4385812571 hasAuthorship W4385812571A5033356745 @default.
• W4385812571 hasAuthorship W4385812571A5059957140 @default.
• W4385812571 hasAuthorship W4385812571A5067962033 @default.
• W4385812571 hasAuthorship W4385812571A5076385283 @default.
• W4385812571 hasAuthorship W4385812571A5089245987 @default.
• W4385812571 hasBestOaLocation W43858125711 @default.
• W4385812571 hasConcept C104317684 @default.
• W4385812571 hasConcept C111798993 @default.
• W4385812571 hasConcept C121608353 @default.
• W4385812571 hasConcept C147447768 @default.
• W4385812571 hasConcept C153911025 @default.
• W4385812571 hasConcept C163688615 @default.
• W4385812571 hasConcept C185592680 @default.
• W4385812571 hasConcept C202751555 @default.
• W4385812571 hasConcept C203014093 @default.
• W4385812571 hasConcept C204232928 @default.
• W4385812571 hasConcept C2775858924 @default.
• W4385812571 hasConcept C2776107976 @default.
• W4385812571 hasConcept C2776415932 @default.
• W4385812571 hasConcept C2776530083 @default.
• W4385812571 hasConcept C2776833033 @default.
• W4385812571 hasConcept C2779013556 @default.
• W4385812571 hasConcept C2779244956 @default.
• W4385812571 hasConcept C2780381497 @default.
• W4385812571 hasConcept C502942594 @default.
• W4385812571 hasConcept C54355233 @default.
• W4385812571 hasConcept C553184892 @default.
• W4385812571 hasConcept C55493867 @default.
• W4385812571 hasConcept C76419328 @default.
• W4385812571 hasConcept C81885089 @default.
• W4385812571 hasConcept C86803240 @default.
• W4385812571 hasConcept C8891405 @default.
• W4385812571 hasConceptScore W4385812571C104317684 @default.
• W4385812571 hasConceptScore W4385812571C111798993 @default.
• W4385812571 hasConceptScore W4385812571C121608353 @default.
• W4385812571 hasConceptScore W4385812571C147447768 @default.
• W4385812571 hasConceptScore W4385812571C153911025 @default.
• W4385812571 hasConceptScore W4385812571C163688615 @default.

• next