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• W4385834739 endingPage "3064" @default.
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• W4385834739 abstract "Several compounds with different physical properties are present in foods, biological components, and environmental samples, and there are cases in which these must be analyzed simultaneously. However, it is difficult to extract compounds with different physical properties from the same sample using a single method. In the present study, we examined the optimal conditions for the QuEChERS extraction of several kinds of compounds from orange juice using design of experiments (DoE) and response surface methodology (RSM) to determine the optimal ratio of organic solvent to sodium chloride. We determined the optimal extraction conditions, which were within the design space, using 100% tetrahydrofuran (THF) as the extraction organic solvent and NaCl:MgSO4 = 75:25 as the salt. The developed LC/MS/MS method using QuEChERS extraction achieved specific detection and precise quantification. Finally, we measured the polyphenols, sterols, and carotenoids in citrus juice using the optimized QuEChERS extraction method before LC/MS/MS analysis. Most of the analytes were quantifiable in orange juice. The optimized method achieved ease of operation, the extraction of analytes from food samples in a short time (within 30 min), minimization of analytical residues, and reliability. The DoE and RSM approach may contribute to better optimization of the extraction conditions for the lowest number of experiments." @default.
• W4385834739 created "2023-08-16" @default.
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• W4385834739 date "2023-08-15" @default.
• W4385834739 modified "2023-09-26" @default.
• W4385834739 title "Optimization of QuEChERS Extraction for Determination of Carotenoids, Polyphenols, and Sterols in Orange Juice Using Design of Experiments and Response Surface Methodology" @default.
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• W4385834739 doi "https://doi.org/10.3390/foods12163064" @default.
• W4385834739 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37628062" @default.
• W4385834739 hasPublicationYear "2023" @default.
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