Matches in SemOpenAlex for { <https://semopenalex.org/work/W4385835269> ?p ?o ?g. }
- W4385835269 endingPage "2065" @default.
- W4385835269 startingPage "2065" @default.
- W4385835269 abstract "Degranulation mediated killing mechanism by NK cells is dependent on store-operated Ca2+ entry (SOCE) and has optimum at moderate intracellular Ca2+ elevations so that partial block of SOCE optimizes the killing process. In this study, we tested the effect of the selective blocker of KCa3.1 channel NS6180 on SOCE and the killing efficiency of NK cells from healthy donors and NK-92 cells against T-ALL cell line Jurkat. Patch-clamp analysis showed that only one-quarter of resting NK cells functionally express KCa3.1 current, which increases 3-fold after activation by interleukins 15 and 2. Nevertheless, blockage of KCa3.1 significantly reduced SOCE and intracellular Ca2+ rise induced by IL-15 or target cell recognition. NS6180 (1 μM) decreased NK degranulation at zero time of coculture with Jurkat cells but already after 1 h, the degranulation reached the same level as in the control. Monitoring of target cell death by flow cytometry and confocal microscopy demonstrated that NS6180 significantly improved the killing ability of NK cells after 1 h in coculture with Jurkat cells and increased the Jurkat cell fraction with apoptotic and necrotic markers. Our data evidence a strong dependence of SOCE on KCa3.1 activity in NK cells and that KCa3.1 specific block can improve NK cytotoxicity." @default.
- W4385835269 created "2023-08-16" @default.
- W4385835269 creator A5010311658 @default.
- W4385835269 creator A5033322392 @default.
- W4385835269 creator A5088801175 @default.
- W4385835269 creator A5092641349 @default.
- W4385835269 date "2023-08-15" @default.
- W4385835269 modified "2023-10-14" @default.
- W4385835269 title "Reduction of Ca2+ Entry by a Specific Block of KCa3.1 Channels Optimizes Cytotoxic Activity of NK Cells against T-ALL Jurkat Cells" @default.
- W4385835269 cites W1549948336 @default.
- W4385835269 cites W1574748667 @default.
- W4385835269 cites W1964963939 @default.
- W4385835269 cites W1982862994 @default.
- W4385835269 cites W1986240989 @default.
- W4385835269 cites W1987101617 @default.
- W4385835269 cites W1990863238 @default.
- W4385835269 cites W1992695016 @default.
- W4385835269 cites W2004762745 @default.
- W4385835269 cites W2006073656 @default.
- W4385835269 cites W2020460437 @default.
- W4385835269 cites W2022069273 @default.
- W4385835269 cites W2027200716 @default.
- W4385835269 cites W2030479182 @default.
- W4385835269 cites W2035798975 @default.
- W4385835269 cites W2041849569 @default.
- W4385835269 cites W2055669282 @default.
- W4385835269 cites W2059321525 @default.
- W4385835269 cites W2060599334 @default.
- W4385835269 cites W2068533560 @default.
- W4385835269 cites W2080077963 @default.
- W4385835269 cites W2090108522 @default.
- W4385835269 cites W2108442939 @default.
- W4385835269 cites W2125404510 @default.
- W4385835269 cites W2128172681 @default.
- W4385835269 cites W2161387630 @default.
- W4385835269 cites W2163230514 @default.
- W4385835269 cites W2164819852 @default.
- W4385835269 cites W2167355269 @default.
- W4385835269 cites W2298503769 @default.
- W4385835269 cites W2329836368 @default.
- W4385835269 cites W2742727395 @default.
- W4385835269 cites W2793903904 @default.
- W4385835269 cites W2794989655 @default.
- W4385835269 cites W2800281194 @default.
- W4385835269 cites W2803004495 @default.
- W4385835269 cites W2806399783 @default.
- W4385835269 cites W2807825632 @default.
- W4385835269 cites W2890393634 @default.
- W4385835269 cites W2907916603 @default.
- W4385835269 cites W2945863457 @default.
- W4385835269 cites W2962791178 @default.
- W4385835269 cites W3083666178 @default.
- W4385835269 cites W3087822810 @default.
- W4385835269 cites W3117850328 @default.
- W4385835269 cites W328083015 @default.
- W4385835269 cites W4200508925 @default.
- W4385835269 doi "https://doi.org/10.3390/cells12162065" @default.
- W4385835269 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37626875" @default.
- W4385835269 hasPublicationYear "2023" @default.
- W4385835269 type Work @default.
- W4385835269 citedByCount "0" @default.
- W4385835269 crossrefType "journal-article" @default.
- W4385835269 hasAuthorship W4385835269A5010311658 @default.
- W4385835269 hasAuthorship W4385835269A5033322392 @default.
- W4385835269 hasAuthorship W4385835269A5088801175 @default.
- W4385835269 hasAuthorship W4385835269A5092641349 @default.
- W4385835269 hasBestOaLocation W43858352691 @default.
- W4385835269 hasConcept C153911025 @default.
- W4385835269 hasConcept C154317977 @default.
- W4385835269 hasConcept C170493617 @default.
- W4385835269 hasConcept C179464577 @default.
- W4385835269 hasConcept C185592680 @default.
- W4385835269 hasConcept C202751555 @default.
- W4385835269 hasConcept C203014093 @default.
- W4385835269 hasConcept C2776090121 @default.
- W4385835269 hasConcept C49802076 @default.
- W4385835269 hasConcept C54355233 @default.
- W4385835269 hasConcept C553184892 @default.
- W4385835269 hasConcept C55493867 @default.
- W4385835269 hasConcept C79879829 @default.
- W4385835269 hasConcept C81885089 @default.
- W4385835269 hasConcept C86803240 @default.
- W4385835269 hasConcept C8891405 @default.
- W4385835269 hasConcept C95444343 @default.
- W4385835269 hasConceptScore W4385835269C153911025 @default.
- W4385835269 hasConceptScore W4385835269C154317977 @default.
- W4385835269 hasConceptScore W4385835269C170493617 @default.
- W4385835269 hasConceptScore W4385835269C179464577 @default.
- W4385835269 hasConceptScore W4385835269C185592680 @default.
- W4385835269 hasConceptScore W4385835269C202751555 @default.
- W4385835269 hasConceptScore W4385835269C203014093 @default.
- W4385835269 hasConceptScore W4385835269C2776090121 @default.
- W4385835269 hasConceptScore W4385835269C49802076 @default.
- W4385835269 hasConceptScore W4385835269C54355233 @default.
- W4385835269 hasConceptScore W4385835269C553184892 @default.
- W4385835269 hasConceptScore W4385835269C55493867 @default.
- W4385835269 hasConceptScore W4385835269C79879829 @default.
- W4385835269 hasConceptScore W4385835269C81885089 @default.