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• W4385837898 abstract "Abstract One of the goals of synthetic biology is to enable the design of arbitrary molecular circuits with programmable inputs and outputs. Such circuits bridge the properties of electronic and natural circuits, processing information in a predictable manner within living cells. Genome editing is a potentially powerful component of synthetic molecular circuits, whether for modulating the expression of a target gene or for stably recording information to genomic DNA. However, programming molecular events such as protein-protein interactions or induced proximity as triggers for genome editing remains challenging. Here we demonstrate a strategy termed P3 editing, which links p rotein- p rotein p roximity to the formation of a functional CRISPR-Cas9 dual-component guide RNA. By engineering the crRNA:tracrRNA interaction, we demonstrate that various known protein-protein interactions, as well as the chemically-induced dimerization of protein domains, can be used to activate prime editing or base editing in human cells. Additionally, we explore how P3 editing can incorporate outputs from ADAR-based RNA sensors, potentially allowing specific RNAs to induce specific genome edits within a larger circuit. Our strategy enhances the controllability of CRISPR-based genome editing, facilitating its use in synthetic molecular circuits deployed in living cells." @default.
• W4385837898 created "2023-08-16" @default.
• W4385837898 creator A5003547752 @default.
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• W4385837898 date "2023-08-14" @default.
• W4385837898 modified "2023-10-17" @default.
• W4385837898 title "A molecular proximity sensor based on an engineered, dual-component guide RNA" @default.
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• W4385837898 doi "https://doi.org/10.1101/2023.08.14.553235" @default.
• W4385837898 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37645782" @default.
• W4385837898 hasPublicationYear "2023" @default.
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