Matches in SemOpenAlex for { <https://semopenalex.org/work/W4385838761> ?p ?o ?g. }
- W4385838761 abstract "Skeletal muscle regeneration is a complex process regulated by many cytokines and growth factors. Among the important signaling pathways regulating the myogenic cell identity are these involving SDF-1 and NOTCH. SDF-1 participates in cell mobilization and acts as an important chemoattractant. NOTCH, on the other hand, controls cell activation and myogenic determination of satellite cells. Knowledge about the interaction between SDF-1 and NOTCH signaling is limited.We analyzed two populations of myogenic cells isolated from mouse skeletal muscle, that is, myoblasts derived from satellite cells (SCs) and muscle interstitial progenitor cells (MIPCs). First, microRNA level changes in response to SDF-1 treatment were analyzed with next-generation sequencing (NGS). Second, myogenic cells, i.e., SC-derived myoblasts and MIPCs were transfected with miRNA mimics, selected on the basis of NGS results, or their inhibitors. Transcriptional changes, as well as proliferation, migration, and differentiation abilities of SC-derived myoblasts and MIPCs, were analyzed in vitro. Naive myogenic potential was assessed in vivo, using subcutaneous engrafts and analysis of cell contribution to regeneration of the skeletal muscles.SDF-1 treatment led to down-regulation of miR10a, miR151, miR425, and miR5100 in myoblasts. Interestingly, miR10a, miR425, and miR5100 regulated the expression of factors involved in the NOTCH signaling pathway, including Dll1, Jag2, and NICD. Furthermore, miR10a, miR425, and miR5100 down-regulated the expression of factors involved in cell migration: Acta1, MMP12, and FAK, myogenic differentiation: Pax7, Myf5, Myod, Mef2c, Myog, Musk, and Myh3. However, these changes did not significantly affect myogenic cell migration or fusion either in vitro or in vivo, except when miR425 was overexpressed, or miR5100 inhibitor was used. These two molecules increased the fusion of MIPCs and myoblasts, respectively. Furthermore, miR425-transfected MIPC transplantation into injured skeletal muscle resulted in more efficient regeneration, compared to control cell transplantation. However, skeletal muscles that were injected with miR10a transfected myoblasts regenerated less efficiently.SDF-1 down-regulates miR10a, miR425, and miR5100, what could affect NOTCH signaling, differentiation of myogenic cells, and their participation in skeletal muscle regeneration." @default.
- W4385838761 created "2023-08-16" @default.
- W4385838761 creator A5015972654 @default.
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- W4385838761 creator A5092642176 @default.
- W4385838761 date "2023-08-15" @default.
- W4385838761 modified "2023-09-26" @default.
- W4385838761 title "SDF-1 and NOTCH signaling in myogenic cell differentiation: the role of miRNA10a, 425, and 5100" @default.
- W4385838761 cites W116011640 @default.
- W4385838761 cites W1819309358 @default.
- W4385838761 cites W1927226147 @default.
- W4385838761 cites W1966786218 @default.
- W4385838761 cites W1980073120 @default.
- W4385838761 cites W1981141119 @default.
- W4385838761 cites W1986433875 @default.
- W4385838761 cites W1988141621 @default.
- W4385838761 cites W1994702815 @default.
- W4385838761 cites W1995906570 @default.
- W4385838761 cites W1998118119 @default.
- W4385838761 cites W2010735936 @default.
- W4385838761 cites W2015185372 @default.
- W4385838761 cites W2017075649 @default.
- W4385838761 cites W2017429680 @default.
- W4385838761 cites W2019921405 @default.
- W4385838761 cites W2026929890 @default.
- W4385838761 cites W2031486950 @default.
- W4385838761 cites W2042736567 @default.
- W4385838761 cites W2053205331 @default.
- W4385838761 cites W2056281268 @default.
- W4385838761 cites W2057289143 @default.
- W4385838761 cites W2059357290 @default.
- W4385838761 cites W2069350886 @default.
- W4385838761 cites W2086331407 @default.
- W4385838761 cites W2089748634 @default.
- W4385838761 cites W2093355627 @default.
- W4385838761 cites W2098290432 @default.
- W4385838761 cites W2112039060 @default.
- W4385838761 cites W2127826417 @default.
- W4385838761 cites W2128722374 @default.
- W4385838761 cites W2134629862 @default.
- W4385838761 cites W2134665278 @default.
- W4385838761 cites W2146138975 @default.
- W4385838761 cites W2157446958 @default.
- W4385838761 cites W2163748946 @default.
- W4385838761 cites W2267893954 @default.
- W4385838761 cites W2302328730 @default.
- W4385838761 cites W2347059048 @default.
- W4385838761 cites W2427154682 @default.
- W4385838761 cites W2523514363 @default.
- W4385838761 cites W2531243522 @default.
- W4385838761 cites W2560481660 @default.
- W4385838761 cites W2592096266 @default.
- W4385838761 cites W2612350007 @default.
- W4385838761 cites W2622669643 @default.
- W4385838761 cites W2770705236 @default.
- W4385838761 cites W2772634406 @default.
- W4385838761 cites W2793567059 @default.
- W4385838761 cites W2811212358 @default.
- W4385838761 cites W2886514991 @default.
- W4385838761 cites W2888397678 @default.
- W4385838761 cites W2894358495 @default.
- W4385838761 cites W2910671651 @default.
- W4385838761 cites W2913486325 @default.
- W4385838761 cites W2922419603 @default.
- W4385838761 cites W2923490702 @default.
- W4385838761 cites W2944869407 @default.
- W4385838761 cites W2947020280 @default.
- W4385838761 cites W2953462016 @default.
- W4385838761 cites W2963667019 @default.
- W4385838761 cites W2970358232 @default.
- W4385838761 cites W2980429396 @default.
- W4385838761 cites W2982388544 @default.
- W4385838761 cites W2996284130 @default.
- W4385838761 cites W3004347194 @default.
- W4385838761 cites W3006228279 @default.
- W4385838761 cites W3012919136 @default.
- W4385838761 cites W3023776828 @default.
- W4385838761 cites W3025279674 @default.
- W4385838761 cites W3026427316 @default.
- W4385838761 cites W3047146087 @default.
- W4385838761 cites W3094017739 @default.
- W4385838761 cites W3098558972 @default.
- W4385838761 cites W3124238398 @default.
- W4385838761 cites W3132668814 @default.
- W4385838761 cites W3158921021 @default.
- W4385838761 cites W3167510105 @default.
- W4385838761 cites W3183111398 @default.