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• W4385855703 abstract "Summary Having tools and resources for independent control of gene expression in two different tissues in the same animal is emerging as a major need, especially in the context of inter-organ communication studies. This type of study is made possible by technologies combining the GAL4/UAS and a second binary expression system such as LexA/LexAop or QF/QUAS. Here, we describe a resource of reagents that facilitate combined use of the GAL4/UAS and a second binary systems in various tissues. Focusing on genes with well-characterized GAL4 expression patterns, we generated a set of more than 40 LexA-GAD and QF2 insertions by CRISPR knock-in and verified their tissue-specificity in larvae. We also built constructs that encode QF2 and LexA-GAD transcription factors in a single vector, with each coding sequence flanked by FRT sites. Following successful integration into the fly genome, the vector generates a single polypeptide, which is separated into the individual drivers by the inclusion of T2A. If desired, one of the two coding regions can then be excised with Flp, resulting in flies that express only QF2 or LexA-GAD. We evaluated both QF2 and LexA-GAD systems for in vivo gene knockdown and are generating a compatible library of transgenic shRNA lines in our custom QUAS and LexAop vectors as a community resource. Together, these QF2/LexA-GAD and QUAS/LexAop vectors and fly lines will provide a new set of tools for researchers who need to activate or repress two different genes in an orthogonal manner in the same animal." @default.
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• W4385855703 date "2023-08-16" @default.
• W4385855703 modified "2023-10-04" @default.
• W4385855703 title "Expanding the Drosophila toolkit for dual control of gene expression" @default.
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• W4385855703 doi "https://doi.org/10.1101/2023.08.15.553399" @default.
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