FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W4385875706> ?p ?o ?g. }

• W4385875706 abstract "Summary Single-cell sequencing has revolutionized our understanding of cellular heterogeneity by providing a micro-level perspective over the past decades. Although heterogeneity is essential for various biological communities, the currently demonstrated platform predominantly focuses on eukaryotic cells without cell walls and their transcriptomics 1,2 , leaving significant gaps in the study of omics from other single biological entities such as bacteria and viruses. Due to the difficulty of isolating and acquiring their DNA 3 , contemporary methodologies for the characterization of generic biological entities remain conspicuously constrained, with low throughput 4 , compromised lysis efficiency 5 , and highly fragmented genomes 6 . Herein, we present the Generic Single Entity Sequencing platform (GSE-Seq), which boasts ample versatility, high throughput, and high coverage, and is enabled by an innovative workflow, addressing the critical challenges in single entities sequencing: (1) one-step manufacturing of massive barcodes, (2) degradable hydrogel-based in situ sample processing and whole genome amplification, (3) integrated in-drop library preparation, (4) compatible long-read sequencing. By GSE-Seq, we have achieved a significant milestone by enabling high-throughput, long-read single-entity profiling of dsDNA and ssDNA from single virus sequencing (SV-seq) and single bacteria sequencing (SB-seq) of the human gut and marine sediment for the first time. Notably, our analysis uncovered previously overlooked viral and bacterial dark matter and phage-host interactions. In summary, the presented conceptually new workflow offers a toolbox based on droplet microfluidics to tackle the persistent challenges in high-throughput profiling to generic applications, which hold immense promise for diverse biological entities, especially hard-to-lyse cells." @default.
• W4385875706 created "2023-08-17" @default.
• W4385875706 creator A5000059613 @default.
• W4385875706 creator A5007585753 @default.
• W4385875706 creator A5008040394 @default.
• W4385875706 creator A5017092011 @default.
• W4385875706 creator A5026619357 @default.
• W4385875706 creator A5032046795 @default.
• W4385875706 creator A5041243214 @default.
• W4385875706 creator A5043327776 @default.
• W4385875706 creator A5047750313 @default.
• W4385875706 creator A5056286171 @default.
• W4385875706 creator A5065818596 @default.
• W4385875706 creator A5073940603 @default.
• W4385875706 creator A5084979131 @default.
• W4385875706 date "2023-08-15" @default.
• W4385875706 modified "2023-10-04" @default.
• W4385875706 title "High-throughput generic single-entity sequencing using droplet microfluidics" @default.
• W4385875706 cites W1939004772 @default.
• W4385875706 cites W1966912927 @default.
• W4385875706 cites W1974288263 @default.
• W4385875706 cites W1975631835 @default.
• W4385875706 cites W2029583307 @default.
• W4385875706 cites W2102212449 @default.
• W4385875706 cites W2125826054 @default.
• W4385875706 cites W2135937351 @default.
• W4385875706 cites W2169899471 @default.
• W4385875706 cites W2245444176 @default.
• W4385875706 cites W2517568995 @default.
• W4385875706 cites W2559325775 @default.
• W4385875706 cites W2620147451 @default.
• W4385875706 cites W2638784546 @default.
• W4385875706 cites W2742647527 @default.
• W4385875706 cites W2765135073 @default.
• W4385875706 cites W2792384873 @default.
• W4385875706 cites W2809646241 @default.
• W4385875706 cites W2940459311 @default.
• W4385875706 cites W2979358136 @default.
• W4385875706 cites W2995649573 @default.
• W4385875706 cites W3013151148 @default.
• W4385875706 cites W3042305844 @default.
• W4385875706 cites W3092429684 @default.
• W4385875706 cites W3104596207 @default.
• W4385875706 cites W3129897473 @default.
• W4385875706 cites W3204872827 @default.
• W4385875706 cites W4281716380 @default.
• W4385875706 cites W4285097835 @default.
• W4385875706 cites W4286510420 @default.
• W4385875706 cites W4288096194 @default.
• W4385875706 cites W4297990241 @default.
• W4385875706 cites W4300861479 @default.
• W4385875706 cites W4313656753 @default.
• W4385875706 cites W4319293985 @default.
• W4385875706 doi "https://doi.org/10.1101/2023.08.15.549386" @default.
• W4385875706 hasPublicationYear "2023" @default.
• W4385875706 type Work @default.
• W4385875706 citedByCount "0" @default.
• W4385875706 crossrefType "posted-content" @default.
• W4385875706 hasAuthorship W4385875706A5000059613 @default.
• W4385875706 hasAuthorship W4385875706A5007585753 @default.
• W4385875706 hasAuthorship W4385875706A5008040394 @default.
• W4385875706 hasAuthorship W4385875706A5017092011 @default.
• W4385875706 hasAuthorship W4385875706A5026619357 @default.
• W4385875706 hasAuthorship W4385875706A5032046795 @default.
• W4385875706 hasAuthorship W4385875706A5041243214 @default.
• W4385875706 hasAuthorship W4385875706A5043327776 @default.
• W4385875706 hasAuthorship W4385875706A5047750313 @default.
• W4385875706 hasAuthorship W4385875706A5056286171 @default.
• W4385875706 hasAuthorship W4385875706A5065818596 @default.
• W4385875706 hasAuthorship W4385875706A5073940603 @default.
• W4385875706 hasAuthorship W4385875706A5084979131 @default.
• W4385875706 hasBestOaLocation W43858757061 @default.
• W4385875706 hasConcept C104317684 @default.
• W4385875706 hasConcept C111919701 @default.
• W4385875706 hasConcept C141231307 @default.
• W4385875706 hasConcept C1491633281 @default.
• W4385875706 hasConcept C157764524 @default.
• W4385875706 hasConcept C171250308 @default.
• W4385875706 hasConcept C177212765 @default.
• W4385875706 hasConcept C187191949 @default.
• W4385875706 hasConcept C192562407 @default.
• W4385875706 hasConcept C2776950831 @default.
• W4385875706 hasConcept C41008148 @default.
• W4385875706 hasConcept C51679486 @default.
• W4385875706 hasConcept C54355233 @default.
• W4385875706 hasConcept C552990157 @default.
• W4385875706 hasConcept C555944384 @default.
• W4385875706 hasConcept C70721500 @default.
• W4385875706 hasConcept C76155785 @default.
• W4385875706 hasConcept C77088390 @default.
• W4385875706 hasConcept C8673954 @default.
• W4385875706 hasConcept C86803240 @default.
• W4385875706 hasConceptScore W4385875706C104317684 @default.
• W4385875706 hasConceptScore W4385875706C111919701 @default.
• W4385875706 hasConceptScore W4385875706C141231307 @default.
• W4385875706 hasConceptScore W4385875706C1491633281 @default.
• W4385875706 hasConceptScore W4385875706C157764524 @default.
• W4385875706 hasConceptScore W4385875706C171250308 @default.
• W4385875706 hasConceptScore W4385875706C177212765 @default.
• W4385875706 hasConceptScore W4385875706C187191949 @default.

• next