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- W4385886438 abstract "BRAF is frequently activated via mutation in human cancer and the RASopathy syndromes; however, for BRAF activation to occur, autoinhibitory interactions between the regulatory and catalytic domains must be relieved. Here, we present a proximity-based NanoBRET (bioluminescence resonance energy transfer) assay for real-time measurement of BRAF autoinhibition in live cells. We describe steps for seeding, transfecting, and replating cells. We then detail procedures for reading the NanoBRET emissions and confirming protein expression. For complete details on the use and execution of this protocol, please refer to Spencer-Smith et al. (2022).1" @default.
- W4385886438 created "2023-08-17" @default.
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- W4385886438 date "2023-09-01" @default.
- W4385886438 modified "2023-10-01" @default.
- W4385886438 title "Protocol for measuring BRAF autoinhibition in live cells using a proximity-based NanoBRET assay" @default.
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- W4385886438 doi "https://doi.org/10.1016/j.xpro.2023.102461" @default.
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