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- W4385954760 abstract "Abstract The lactic acid bacteria, including Latilactobacillus sakei and Latilactobacillus curvatus , have been widely studied for their preventive and therapeutic effects. In this study, the underlying mechanism of action for the antioxidant and immunostimulatory effects of two strains of heat-treated paraprobiotics was examined. Heat-treated Lact. sakei KU15041 and Lact. curvatus KU15003 showed higher radical scavenging activity in both the 2-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and 2,2-diphenyl-1-picryl-hydrazyl (DPPH) assays than the commercial probiotic strain LGG. In addition, treatment with these two strains exhibited immunostimulatory effects in RAW 264.7 macrophages, with Lact. curvatus KU15003 showing a slightly higher effect. Additionally, they promoted phagocytosis and NO production in RAW 264.7 cells without any cytotoxicity. Moreover, the expression of tumor necrosis factor-α, interleukin (IL)-1β, and IL-6 was upregulated. These strains resulted in an increased expression of inducible nitric oxide synthase and cyclooxygenase-2. Moreover, the nuclear factor-κB and mitogen-activated protein kinase signaling pathways were stimulated by these strains. These findings suggest the potential of using Lact. sakei KU15041 and Lact. curvatus KU15003 in food or by themselves as probiotics with antioxidant and immune-enhancing properties." @default.
- W4385954760 created "2023-08-18" @default.
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- W4385954760 date "2023-08-17" @default.
- W4385954760 modified "2023-10-05" @default.
- W4385954760 title "Antioxidant and immunostimulatory effect of heat-treated paraprobiotics Latilactobacillus sakei KU15041 and Latilactobacillus curvatus KU15003" @default.
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- W4385954760 doi "https://doi.org/10.21203/rs.3.rs-3253743/v1" @default.
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