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• W4385969695 abstract "The CRISPR JournalVol. 6, No. 4 AnnouncementFree AccessRosalind Franklin Society Proudly Announces the 2022 Award Recipient for The CRISPR JournalNicole F. BrackettNicole F. BrackettInBio, Charlottesville, VA, USASearch for more papers by this authorPublished Online:14 Aug 2023https://doi.org/10.1089/crispr.2023.29162.rfs2022AboutSectionsPDF/EPUB Permissions & CitationsPermissionsDownload CitationsTrack CitationsAdd to favorites Back To Publication ShareShare onFacebookTwitterLinked InRedditEmail The Rosalind Franklin Society (RFS), in partnership with Mary Ann Liebert, Inc., publishers, enthusiastically congratulate our distinguished recipient of the 2022 annualRFS Award in Sciencefor this journal, which recognizes the outstanding research and published work of women and underrepresented minority scientists, physicians, and engineers.Nicole F. Brackett, Brian W. Davis, Mazhar Adli, Anna Pomés, and Martin D. Chapman, “Evolutionary Biology and Gene Editing of Cat Allergen, Fel d 1,” The CRISPR Journal 5, no. 2 (April 2022): 213–223, http://doi.org/10.1089/crispr.2021.0101.AbstractAllergy to domestic cat affects up to 15% of the population and sensitization to cat allergen is associated with asthma. Despite the pervasiveness of cat allergic disease, current treatments have limited impact. Here, we present a bioinformatics analysis of the major cat allergen, Fel d 1, and demonstrate proof-of-principle for CRISPR gene editing of the allergen. Sequence and structural analyses of Fel d 1 from 50 domestic cats identified conserved coding regions in the genes (CH1 and CH2) suitable for CRISPR editing. Comparative analyses of Fel d 1 and orthologous sequences from eight exotic felid species determined relatively low sequence identities for CH1 and CH2, and implied that the allergen may be non-essential for cats given the apparent lack of evolutionary conservation. In vitro knockouts of domestic cat Fel d 1 using CRISPR-Cas9 yielded editing efficiencies of up to 55% and found no evidence of editing at predicted potential off-target sites. Taken together, our data indicate that Fel d 1 is both a rational and viable candidate for gene deletion, which may profoundly benefit cat allergy sufferers by removing the major allergen at the source.BiosketchDr. Nicole Brackett is a senior scientist at InBio, a small biotechnology company that specializes in allergen detection and biomanufacturing. Dr. Brackett earned her PhD in biomedical engineering from the University of 2Virginia, where her dissertation research focused on the molecular mechanisms of blood cell adhesion and the contribution of platelets to coagulation. Since joining InBio as a postdoctoral scientist in 2018 and advancing to senior scientist in 2021, Dr. Brackett's work has centered on the major cat allergen, Fel d 1. Her research efforts include comparative bioinformatics analyses of Fel d 1 across 37 species of cats as an approach to gain insights into the evolution and potential function of the allergen. Additionally, Dr. Brackett is applying state-of-the-art CRISPR technology for the targeted deletion of the Fel d 1 genes. If successful, this would be the only solution to cat allergy that unambiguously removes the major allergen from the animal, which may have significant, verifiable health benefits for cat allergic patients. Dr. Brackett has proudly shared her CRISPR cat research in scientific publications and meetings, and she hopes that the work will drive the development of novel treatments in allergy medicine.FiguresReferencesRelatedDetails Volume 6Issue 4Aug 2023 InformationCopyright 2023, Mary Ann Liebert, Inc., publishersTo cite this article:Nicole F. Brackett.Rosalind Franklin Society Proudly Announces the 2022 Award Recipient for The CRISPR Journal.The CRISPR Journal.Aug 2023.401-401.http://doi.org/10.1089/crispr.2023.29162.rfs2022Published in Volume: 6 Issue 4: August 14, 2023PDF download" @default.
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