Matches in SemOpenAlex for { <https://semopenalex.org/work/W4385978711> ?p ?o ?g. }
- W4385978711 endingPage "508" @default.
- W4385978711 startingPage "508" @default.
- W4385978711 abstract "Molecular cloning and controlled expression remain challenging when the target gene encodes a protein that is toxic to the host. We developed a set of multi-layer control systems to enable cloning of genes encoding proteins known to be highly toxic in Escherichia coli and other bacteria. The different multi-layer control systems combine a promoter–operator system on a transcriptional level with a riboswitch for translational control. Additionally, replicational control is ensured by using a strain that reduces the plasmid copy number. The use of weaker promoters (such as PBAD or PfdeA) in combination with the effective theophylline riboswitch is essential for cloning genes that encode notoriously toxic proteins that directly target translation and transcription. Controlled overexpression is possible, allowing the system to be used for evaluating in vivo effects of the toxin. Systems with a stronger promoter can be used for successful overexpression and purification of the desired protein but are limited to toxins that are more moderate and do not interfere with their own production." @default.
- W4385978711 created "2023-08-19" @default.
- W4385978711 creator A5035569738 @default.
- W4385978711 creator A5038576604 @default.
- W4385978711 creator A5042854852 @default.
- W4385978711 creator A5052942121 @default.
- W4385978711 creator A5092661149 @default.
- W4385978711 date "2023-08-18" @default.
- W4385978711 modified "2023-09-30" @default.
- W4385978711 title "A Multi-Layer-Controlled Strategy for Cloning and Expression of Toxin Genes in Escherichia coli" @default.
- W4385978711 cites W1460246699 @default.
- W4385978711 cites W1511273208 @default.
- W4385978711 cites W1565204025 @default.
- W4385978711 cites W1965859342 @default.
- W4385978711 cites W1997212522 @default.
- W4385978711 cites W1999442315 @default.
- W4385978711 cites W2011634515 @default.
- W4385978711 cites W2018289835 @default.
- W4385978711 cites W2021539568 @default.
- W4385978711 cites W2032619610 @default.
- W4385978711 cites W2047946060 @default.
- W4385978711 cites W2053935595 @default.
- W4385978711 cites W2054613021 @default.
- W4385978711 cites W2055932799 @default.
- W4385978711 cites W2069477714 @default.
- W4385978711 cites W2074425367 @default.
- W4385978711 cites W2083873972 @default.
- W4385978711 cites W2088892367 @default.
- W4385978711 cites W2096233452 @default.
- W4385978711 cites W2100837269 @default.
- W4385978711 cites W2101108802 @default.
- W4385978711 cites W2101828342 @default.
- W4385978711 cites W2102169469 @default.
- W4385978711 cites W2103722640 @default.
- W4385978711 cites W2112394205 @default.
- W4385978711 cites W2115328763 @default.
- W4385978711 cites W2115419697 @default.
- W4385978711 cites W2115601383 @default.
- W4385978711 cites W2125641272 @default.
- W4385978711 cites W2132417094 @default.
- W4385978711 cites W2138820253 @default.
- W4385978711 cites W2148046194 @default.
- W4385978711 cites W2160084442 @default.
- W4385978711 cites W2166744717 @default.
- W4385978711 cites W2312343991 @default.
- W4385978711 cites W2327442225 @default.
- W4385978711 cites W2763797050 @default.
- W4385978711 cites W2786689114 @default.
- W4385978711 cites W2800740738 @default.
- W4385978711 cites W2949406955 @default.
- W4385978711 cites W3001366927 @default.
- W4385978711 cites W3122438247 @default.
- W4385978711 cites W4317752036 @default.
- W4385978711 doi "https://doi.org/10.3390/toxins15080508" @default.
- W4385978711 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37624265" @default.
- W4385978711 hasPublicationYear "2023" @default.
- W4385978711 type Work @default.
- W4385978711 citedByCount "0" @default.
- W4385978711 crossrefType "journal-article" @default.
- W4385978711 hasAuthorship W4385978711A5035569738 @default.
- W4385978711 hasAuthorship W4385978711A5038576604 @default.
- W4385978711 hasAuthorship W4385978711A5042854852 @default.
- W4385978711 hasAuthorship W4385978711A5052942121 @default.
- W4385978711 hasAuthorship W4385978711A5092661149 @default.
- W4385978711 hasBestOaLocation W43859787111 @default.
- W4385978711 hasConcept C101762097 @default.
- W4385978711 hasConcept C104317684 @default.
- W4385978711 hasConcept C107618985 @default.
- W4385978711 hasConcept C121050878 @default.
- W4385978711 hasConcept C138885662 @default.
- W4385978711 hasConcept C148625055 @default.
- W4385978711 hasConcept C150194340 @default.
- W4385978711 hasConcept C179926584 @default.
- W4385978711 hasConcept C194993378 @default.
- W4385978711 hasConcept C199360897 @default.
- W4385978711 hasConcept C22744801 @default.
- W4385978711 hasConcept C33161422 @default.
- W4385978711 hasConcept C40767141 @default.
- W4385978711 hasConcept C41008148 @default.
- W4385978711 hasConcept C41895202 @default.
- W4385978711 hasConcept C54355233 @default.
- W4385978711 hasConcept C547475151 @default.
- W4385978711 hasConcept C70721500 @default.
- W4385978711 hasConcept C86803240 @default.
- W4385978711 hasConceptScore W4385978711C101762097 @default.
- W4385978711 hasConceptScore W4385978711C104317684 @default.
- W4385978711 hasConceptScore W4385978711C107618985 @default.
- W4385978711 hasConceptScore W4385978711C121050878 @default.
- W4385978711 hasConceptScore W4385978711C138885662 @default.
- W4385978711 hasConceptScore W4385978711C148625055 @default.
- W4385978711 hasConceptScore W4385978711C150194340 @default.
- W4385978711 hasConceptScore W4385978711C179926584 @default.
- W4385978711 hasConceptScore W4385978711C194993378 @default.
- W4385978711 hasConceptScore W4385978711C199360897 @default.
- W4385978711 hasConceptScore W4385978711C22744801 @default.
- W4385978711 hasConceptScore W4385978711C33161422 @default.
- W4385978711 hasConceptScore W4385978711C40767141 @default.
- W4385978711 hasConceptScore W4385978711C41008148 @default.