Matches in SemOpenAlex for { <https://semopenalex.org/work/W4386001002> ?p ?o ?g. }
- W4386001002 abstract "Abstract A universal biochemical signal for bacterial cell-cell communication could facilitate programming dynamic responses in diverse bacterial consortia. However, the classical quorum sensing paradigm is that gram-negative and gram-positive bacteria generally communicate via homoserine lactones (HSL) or oligopeptide molecular signals, respectively, to elicit population responses. Here, we create synthetic HSL sensors for gram-positive Bacillus subtilis 168 using allosteric LuxR-type regulators (RpaR, LuxR, RhlR, and CinR) and synthetic promoters. Promoters were combinatorially designed from different sequence elements (–35, –16, –10, and transcriptional start regions). We quantified the effects of these combinatorial promoters on sensor activity and determined how regulator expression affects its activation, achieving up to 293-fold activation. Using statistical design of experiments, we identified significant effects of promoter regions and pairwise interactions on sensor activity, which helped to understand the sequence-function relationships for synthetic promoter design. We present the first known set of functional HSL sensors (≥ 20-fold dynamic range) in B. subtilis for four different HSL chemical signals: p -coumaroyl-HSL, 3-oxohexanoyl-HSL, n -butyryl-HSL, and n -(3-hydroxytetradecanoyl)-HSL. This set of synthetic HSL sensors for a gram-positive bacterium can pave the way for designable interspecies communication within microbial consortia." @default.
- W4386001002 created "2023-08-20" @default.
- W4386001002 creator A5023242554 @default.
- W4386001002 creator A5036971797 @default.
- W4386001002 creator A5053254989 @default.
- W4386001002 creator A5063980979 @default.
- W4386001002 creator A5085820792 @default.
- W4386001002 date "2023-08-18" @default.
- W4386001002 modified "2023-10-12" @default.
- W4386001002 title "Synthetic Homoserine Lactone Sensors for Gram-Positive<i>Bacillus subtilis</i>using LuxR-type Regulators" @default.
- W4386001002 cites W1899647260 @default.
- W4386001002 cites W1968549820 @default.
- W4386001002 cites W1977624796 @default.
- W4386001002 cites W1979046613 @default.
- W4386001002 cites W1979197349 @default.
- W4386001002 cites W1982228455 @default.
- W4386001002 cites W1987043663 @default.
- W4386001002 cites W1989147588 @default.
- W4386001002 cites W1991411135 @default.
- W4386001002 cites W1993546274 @default.
- W4386001002 cites W1999018402 @default.
- W4386001002 cites W2008867005 @default.
- W4386001002 cites W2009663387 @default.
- W4386001002 cites W2009816989 @default.
- W4386001002 cites W2011885319 @default.
- W4386001002 cites W2015007275 @default.
- W4386001002 cites W2020044960 @default.
- W4386001002 cites W2027576577 @default.
- W4386001002 cites W2031284066 @default.
- W4386001002 cites W2047245447 @default.
- W4386001002 cites W2047360542 @default.
- W4386001002 cites W2054173531 @default.
- W4386001002 cites W2065833295 @default.
- W4386001002 cites W2083456183 @default.
- W4386001002 cites W2083983731 @default.
- W4386001002 cites W2085961461 @default.
- W4386001002 cites W2090609904 @default.
- W4386001002 cites W2098626852 @default.
- W4386001002 cites W2099090468 @default.
- W4386001002 cites W2100955083 @default.
- W4386001002 cites W2110510425 @default.
- W4386001002 cites W2111173827 @default.
- W4386001002 cites W2112090747 @default.
- W4386001002 cites W2116598242 @default.
- W4386001002 cites W2118521369 @default.
- W4386001002 cites W2121748999 @default.
- W4386001002 cites W2121806138 @default.
- W4386001002 cites W2121988991 @default.
- W4386001002 cites W2123467717 @default.
- W4386001002 cites W2123992133 @default.
- W4386001002 cites W2126922170 @default.
- W4386001002 cites W2131869707 @default.
- W4386001002 cites W2132828256 @default.
- W4386001002 cites W2135986228 @default.
- W4386001002 cites W2138835511 @default.
- W4386001002 cites W2140405224 @default.
- W4386001002 cites W2141564052 @default.
- W4386001002 cites W2145352273 @default.
- W4386001002 cites W2161466984 @default.
- W4386001002 cites W2163269586 @default.
- W4386001002 cites W2172162088 @default.
- W4386001002 cites W2400520117 @default.
- W4386001002 cites W2463822315 @default.
- W4386001002 cites W2472136907 @default.
- W4386001002 cites W2511941815 @default.
- W4386001002 cites W2736894609 @default.
- W4386001002 cites W2766140525 @default.
- W4386001002 cites W2788593837 @default.
- W4386001002 cites W2795239381 @default.
- W4386001002 cites W2811477513 @default.
- W4386001002 cites W2899198283 @default.
- W4386001002 cites W2924530200 @default.
- W4386001002 cites W2931132537 @default.
- W4386001002 cites W2931314706 @default.
- W4386001002 cites W2952214797 @default.
- W4386001002 cites W2954536068 @default.
- W4386001002 cites W2959865871 @default.
- W4386001002 cites W2970696079 @default.
- W4386001002 cites W2979347585 @default.
- W4386001002 cites W3042674204 @default.
- W4386001002 cites W4230336272 @default.
- W4386001002 doi "https://doi.org/10.1101/2023.08.17.553781" @default.
- W4386001002 hasPublicationYear "2023" @default.
- W4386001002 type Work @default.
- W4386001002 citedByCount "0" @default.
- W4386001002 crossrefType "posted-content" @default.
- W4386001002 hasAuthorship W4386001002A5023242554 @default.
- W4386001002 hasAuthorship W4386001002A5036971797 @default.
- W4386001002 hasAuthorship W4386001002A5053254989 @default.
- W4386001002 hasAuthorship W4386001002A5063980979 @default.
- W4386001002 hasAuthorship W4386001002A5085820792 @default.
- W4386001002 hasBestOaLocation W43860010021 @default.
- W4386001002 hasConcept C101762097 @default.
- W4386001002 hasConcept C104317684 @default.
- W4386001002 hasConcept C118687296 @default.
- W4386001002 hasConcept C14036430 @default.
- W4386001002 hasConcept C144024400 @default.
- W4386001002 hasConcept C149923435 @default.
- W4386001002 hasConcept C150194340 @default.
- W4386001002 hasConcept C191908910 @default.