Matches in SemOpenAlex for { <https://semopenalex.org/work/W4386002890> ?p ?o ?g. }
- W4386002890 endingPage "184" @default.
- W4386002890 startingPage "169" @default.
- W4386002890 abstract "Degenerative intervertebral disc disease is a common source of chronic pain and reduced quality of life in people over the age of 40. While degeneration occurs throughout the disc, it most often initiates in the nucleus pulposus (NP). Minimally invasive delivery of NP cells within hydrogels that can restore and maintain the disc height while regenerating the damaged NP tissue is a promising treatment strategy for this condition. Towards this goal, a biohybrid ABA dimethacrylate triblock copolymer was synthesized, possessing a lower critical solution temperature below 37 °C and which contained as its central block an MMP-degradable peptide flanked by poly(trimethylene carbonate) blocks bearing pendant oligoethylene glycol groups. This triblock prepolymer was used to form macroporous NP cell-laden hydrogels via redox initiated (ammonium persulfate/sodium bisulfite) crosslinking, with or without the inclusion of thiolated chondroitin sulfate. The resulting macroporous hydrogels had water and mechanical properties similar to those of human NP tissue and were mechanically resilient. The hydrogels supported NP cell attachment and growth over 28 days in hypoxic culture. In hydrogels prepared with the triblock copolymer but without the chondroitin sulfate the NP cells were distributed homogeneously throughout in clusters and deposited collagen type II and sulfated glycosaminoglycans but not collagen type I. This hydrogel formulation warrants further investigation as a cell delivery vehicle to regenerate degenerated NP tissue. The intervertebral disc between the vertebral bones of the spine consists of three regions: a gel-like central nucleus pulposus (NP) within the annulus fibrosis, and bony endplates. Degeneration of the intervertebral disc is a source of chronic pain in the elderly and most commonly initiates in the NP. Replacement of degenerated NP tissue with a NP cell-laden hydrogel is a promising treatment strategy. Herein we demonstrate that a crosslinkable polymer with a lower critical solution temperature below 37 °C can be used to form macroporous hydrogels for this purpose. The hydrogels are capable of supporting NP cells, which deposit collagen II and sulfated glycosaminoglycans, while also possessing mechanical properties matching those of human NP tissue." @default.
- W4386002890 created "2023-08-20" @default.
- W4386002890 creator A5012249569 @default.
- W4386002890 creator A5062100501 @default.
- W4386002890 date "2023-10-01" @default.
- W4386002890 modified "2023-10-08" @default.
- W4386002890 title "In situ forming macroporous biohybrid hydrogel for nucleus pulposus cell delivery" @default.
- W4386002890 cites W1433345469 @default.
- W4386002890 cites W1894826341 @default.
- W4386002890 cites W1964652258 @default.
- W4386002890 cites W1968946493 @default.
- W4386002890 cites W1970751992 @default.
- W4386002890 cites W1970982790 @default.
- W4386002890 cites W1975045353 @default.
- W4386002890 cites W1989439022 @default.
- W4386002890 cites W1991332628 @default.
- W4386002890 cites W1992647292 @default.
- W4386002890 cites W1994318485 @default.
- W4386002890 cites W1996144874 @default.
- W4386002890 cites W1998289682 @default.
- W4386002890 cites W2007078947 @default.
- W4386002890 cites W2007413067 @default.
- W4386002890 cites W2008658133 @default.
- W4386002890 cites W2010775152 @default.
- W4386002890 cites W2019265265 @default.
- W4386002890 cites W2019958066 @default.
- W4386002890 cites W2022106720 @default.
- W4386002890 cites W2024866505 @default.
- W4386002890 cites W2028368206 @default.
- W4386002890 cites W2030903482 @default.
- W4386002890 cites W2043146067 @default.
- W4386002890 cites W2050358873 @default.
- W4386002890 cites W2050475565 @default.
- W4386002890 cites W2054992092 @default.
- W4386002890 cites W2064003107 @default.
- W4386002890 cites W2071763312 @default.
- W4386002890 cites W2071993171 @default.
- W4386002890 cites W2075076411 @default.
- W4386002890 cites W2075589379 @default.
- W4386002890 cites W2082683336 @default.
- W4386002890 cites W2084492181 @default.
- W4386002890 cites W2090750279 @default.
- W4386002890 cites W2092082033 @default.
- W4386002890 cites W2099748839 @default.
- W4386002890 cites W2111763891 @default.
- W4386002890 cites W2115974053 @default.
- W4386002890 cites W2124094737 @default.
- W4386002890 cites W2130257778 @default.
- W4386002890 cites W2158103052 @default.
- W4386002890 cites W2166369828 @default.
- W4386002890 cites W2206958686 @default.
- W4386002890 cites W2230713678 @default.
- W4386002890 cites W2327343902 @default.
- W4386002890 cites W2334664295 @default.
- W4386002890 cites W2395552044 @default.
- W4386002890 cites W2440884008 @default.
- W4386002890 cites W2501271455 @default.
- W4386002890 cites W2536309485 @default.
- W4386002890 cites W2573157670 @default.
- W4386002890 cites W2575416282 @default.
- W4386002890 cites W2589988169 @default.
- W4386002890 cites W2595588520 @default.
- W4386002890 cites W2596972615 @default.
- W4386002890 cites W2605815679 @default.
- W4386002890 cites W2716386218 @default.
- W4386002890 cites W2737846455 @default.
- W4386002890 cites W2767086944 @default.
- W4386002890 cites W2769840171 @default.
- W4386002890 cites W2771471060 @default.
- W4386002890 cites W2781605224 @default.
- W4386002890 cites W2888636742 @default.
- W4386002890 cites W2893196262 @default.
- W4386002890 cites W2924853573 @default.
- W4386002890 cites W2938243730 @default.
- W4386002890 cites W2944788953 @default.
- W4386002890 cites W3014581123 @default.
- W4386002890 cites W3080689362 @default.
- W4386002890 cites W3187987304 @default.
- W4386002890 cites W3201325635 @default.
- W4386002890 cites W3216522192 @default.
- W4386002890 cites W4200604870 @default.
- W4386002890 cites W4211261770 @default.
- W4386002890 cites W4298184888 @default.
- W4386002890 doi "https://doi.org/10.1016/j.actbio.2023.08.029" @default.
- W4386002890 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/37598793" @default.
- W4386002890 hasPublicationYear "2023" @default.
- W4386002890 type Work @default.
- W4386002890 citedByCount "0" @default.
- W4386002890 crossrefType "journal-article" @default.
- W4386002890 hasAuthorship W4386002890A5012249569 @default.
- W4386002890 hasAuthorship W4386002890A5062100501 @default.
- W4386002890 hasBestOaLocation W43860028901 @default.
- W4386002890 hasConcept C105702510 @default.
- W4386002890 hasConcept C108586683 @default.
- W4386002890 hasConcept C12554922 @default.
- W4386002890 hasConcept C136229726 @default.
- W4386002890 hasConcept C153074725 @default.
- W4386002890 hasConcept C185592680 @default.